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Two active monomers, compositions and applications for treating esophageal cancer

A technology of active monomer and esophageal cancer, which is applied in the field of traditional Chinese medicine, can solve the problems that there is no ratio relationship between active monomer formulas, the research on the mechanism of action of esophageal cancer cells is not systematic, and there is no report on the most active monomer of esophageal cancer. , to achieve the effect of prevention and treatment of esophageal cancer, broad market application prospects, and large market potential

Active Publication Date: 2022-03-01
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the most active monomers in pangolin and Chonglou against esophageal cancer, and there is no proportional relationship between the active monomer formulas, and the research on the mechanism of action of drugs on esophageal cancer cells is not systematic

Method used

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  • Two active monomers, compositions and applications for treating esophageal cancer
  • Two active monomers, compositions and applications for treating esophageal cancer
  • Two active monomers, compositions and applications for treating esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: the preparation of pangolin active monomer

[0060] 1.1 Extraction method

[0061] (1) Alcohol extraction: Weigh the pangolin, soak it in 95% (v / v) ethanol solution (1g:7ml) at room temperature for 6 days, filter it with suction, discard the filter residue, concentrate, freeze-dry in vacuum at -80°C to obtain a brown yellow solid;

[0062] (2) Extraction: dissolve the brown-yellow solid in ultrapure water (mass volume ratio: 1g: 50ml), and use different polar solvents such as petroleum ether, chloroform, ethyl acetate, and n-butanol to extract in a separating funnel. , the five-part extract (petroleum ether, chloroform, ethyl acetate, n-butanol, water) was rotary evaporated, and vacuum-80°C was freeze-dried to obtain a solid. The activity was tested by MTT method, and the results showed that the n-butanol part of pangolinus was active the strongest;

[0063] (3) macroporous resin chromatographic separation: the solid of n-butanol part is dissolved in ul...

Embodiment 2

[0073] Embodiment 2: the preparation of the active monomer of heavy building

[0074] 2.1 Extraction method

[0075] (1) Alcohol extraction: Weigh the chrysanthemum, soak it in 95% (v / v) ethanol solution (mass volume ratio: 1g:7ml) at room temperature for 6 days, filter with suction, discard the filter residue, concentrate, and freeze-dry in vacuum at -80°C to obtain Brown yellow solid;

[0076] (2) Extraction: dissolve the brown-yellow solid in ultrapure water (mass volume ratio: 1g: 50ml), and use different polar solvents such as petroleum ether, chloroform, ethyl acetate, and n-butanol to extract in a separating funnel. , the five-part extract (petroleum ether, chloroform, ethyl acetate, n-butanol, water) was rotary evaporated, and vacuum-80°C was freeze-dried to obtain a solid. The activity was tested by the MTT method, and the results showed that the n-butanol part of the heavy building The most active;

[0077] (3) Chromatographic separation of macroporous resin: the ...

Embodiment 3

[0087] Embodiment 3: Composition for treating esophageal cancer

[0088] The MTT method was used to detect the effects of the active monomers of pangolinus and polyphylla on common esophageal cancer cells (Eca109, EC9706, TE-1, EC-1). The specific methods are as follows:

[0089] 1) The cells in the logarithmic growth phase were digested, centrifuged, resuspended, counted, and inoculated in a 96-well plate, with 3 duplicate wells, 1.0×10 4 cells / well, 200 μl / well, Eca109, TE-1, EC-1 cells were cultured using RPMI1640 medium, EC9706 were cultured using DMEM medium, containing 10% FBS in the medium, and the medium used for the cells of the present invention followed this rule;

[0090] 2) Incubate in an incubator, 5% CO 2 , 37°C, saturated humidity, take it out after 24 hours, discard the upper layer culture solution, set up blank group and drug-dosing group, replace the blank group with 10% FBS medium, and the drug-dosed group replace with 10% FBS medium containing working con...

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Abstract

The invention discloses two kinds of active monomers for treating esophageal cancer and their compositions and applications. The technical scheme is that the invention uses cell models, is guided by activity tracking, and adopts modern methods such as alcohol extraction, chromatography and high performance liquid chromatography. Extraction and separation technology, extracting active monomers from two traditional Chinese medicines with anti-cancer activity, Chuanshanlong and Zhonglou, and using chemical spectroscopy to speculate on the molecular composition and determine the molecular structure of each monomer, and determine the individual monomers by the baseline equal ratio increase and decrease method. According to the dosage ratio of the body, it is combined into a composition for treating esophageal cancer, and then the active monomer and the composition are applied to a variety of esophageal cancer cells to study its mechanism of action on esophageal cancer. The two active monomers and their compositions provided by the invention are all derived from the extraction of natural medicines, are pure traditional Chinese medicine preparations, have the functions of preventing and treating esophageal cancer, and have great market potential and broad market application prospects.

Description

technical field [0001] The present invention relates to two kinds of active monomers for treating esophageal cancer and their compositions and applications, in particular to active monomers extracted from two traditional Chinese medicines, Chuanshanlong and Chonglou, and the composition for treating esophageal cancer, belonging to traditional Chinese medicine technology field. Background technique [0002] The "2018 Global Cancer Statistics Report" shows that esophageal cancer ranks seventh in the incidence of malignant tumors and sixth in the overall death of malignant tumors. It is a disease that seriously endangers human health and brings a heavy economic burden to society and families. The treatment of esophageal cancer with traditional Chinese medicine can enhance the immunity of patients, relieve the pain of patients, and improve their quality of life. Although there are prescriptions and medicines for treating esophageal cancer in the laboratory and clinical practice,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00A61K31/7048A61P35/00
CPCC07J71/0005A61K31/7048A61P35/00
Inventor 司富春王文彬司高
Owner HENAN UNIV OF CHINESE MEDICINE
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