Fed-batch method for improving fermentation yield of teicoplanin

Abstract

The invention discloses a fed-batch method for improving the fermentation yield of teicoplanin, and belongs to the technical field of biological fermentation. The fermentation mode of the teicoplaninis fed-batch fermentation, the feeding mode is fed-batch feeding, the fed-batch substance is a sodium acetate solution, the fed-batch feeding time is from the first logarithmic phase of fed-batch fermentation to the end of the fermentation, and the feeding speed is gradually reduced in the fed-batch feeding process. According to the fed-batch method provided by the invention, the feeding speed iscontrolled for feeding the sodium acetate solution from the first logarithmic phase to the fermentation termination phase of teicoplanin fermentation, so that the fermentation yield of the teicoplaninis remarkably increased and can reach 4.8 g / L. Meanwhile, compared with a fed-batch carbon source, the fed-batch sodium acetate solution does not cause a feedback inhibition effect, and the problemsof insufficient fermentation unit sequent and bubbling and liquid escaping of feed liquid in the later fermentation period are also avoided; and the fed-batch method is low in cost, simple in process,easy to popularize and easy to use in large-scale production.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a feeding method for improving the fermentation yield of teicoplanin. Background technique [0002] Teicoplanin, also known as pepticomycin, is a new glycopeptide antibiotic produced by Actinoplanes teichomyceticus through biological fermentation. It is another treatment for severe Gram's after vancomycin. Positive bacterial infection is the most effective weapon against methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE). [0003] The relative molecular mass of teicoplanin is about 1900, which consists of 7 molecules of amino acids and 3 molecules of sugar groups and acyl fatty acid side chains. The main active ingredient is TA2, which is composed of 5 compounds with very similar chemical structures (TA2-1, TA2-2, TA2-3, TA2-4 and TA2-5), and the difference of the 5 components is only in the ...

AI Technical Summary

Problems solved by technology

Although the existing technology is the Chinese invention patent with the application number 201410729473.5, by adding the feed medium to the fermentation medium during the fermentation process, the factors that inhibit the secondary metabolic pathway of teicoplanin can be reduced, and the teicoplanin can be improved. The yield of laning fermentation, but this method is costly, and it is prone to the problem of feedback inhibition effect caused by foaming and escaping of feed liquid in the later stage of fermentation, adding glucose and other carbon sources

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