Preparation method of pollen pini complex peptide
A compound peptide and pine flower technology, applied in the field of food and health food, can solve the problems of wasting time, single function and efficacy of health products, and achieve the effect of wide market space, speeding up the transmission and absorption of nutrients, and sufficient response.
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Embodiment 1
[0029] Embodiment 1: a kind of pine flower complex peptide preparation method comprises the steps:
[0030] 1) Heat treatment: Weigh 30 parts of broken wall pine pollen, put it in a boiling water bath and cook for 10 minutes, then take out the treated broken wall pine pollen;
[0031] 2) Pour the broken wall pine pollen obtained in step 1) into a blender, mix according to the ratio of xanthan gum: water=3: 10000 to obtain a xanthan gum solution, add 50 parts of the xanthan gum solution to the blender and adjust to The substrate concentration is 30%. At a temperature of 45°C, use xanthan gum and broken-walled pine pollen grains to form a micro-reaction bed. Adjust the pH of the solution to 8 with alkali and fully stir it with a mixer for 30 minutes;
[0032]3) Add 0.03% trypsin and 0.05% bromelain to the mixed solution obtained in step 2), the concentration of the compound enzyme is 30 mg / g protein, and at a temperature of 50°C, put the mixed solution and the compound enzyme in...
Embodiment 2
[0039] Embodiment 2: a kind of pine flower compound peptide preparation method comprises the steps:
[0040] 1) Heat treatment: Weigh 40 parts of broken wall pine pollen and put it in a boiling water bath and cook for 35 minutes, then take out the treated broken wall pine pollen;
[0041] 2) Pour the broken wall pine pollen obtained in step 1) into a blender, mix according to the ratio of xanthan gum: water=3: 10000 to obtain a xanthan gum solution, add 60 parts of the xanthan gum solution to the blender and adjust to The substrate concentration is 40%. At a temperature of 45°C, use xanthan gum and broken-walled pine pollen to form a micro-reaction bed. Use alkali to adjust the pH of the solution to 8.5, and fully stir with a mixer for 45 minutes;
[0042] 3) Add trypsin 0.045% and bromelain 0.065% to the mixed solution obtained in step 2), the concentration of the compound enzyme is 35mg / g protein, and at a temperature of 55°C, the mixed solution and the compound enzyme are f...
Embodiment 3
[0049] Embodiment 3: a kind of pine flower complex peptide preparation method comprises the following steps:
[0050] 1) Heat treatment: Weigh 50 parts of broken-walled pine pollen and put it in a boiling water bath for 60 minutes, then take out the treated broken-walled pine pollen;
[0051] 2) Pour the broken wall pine pollen obtained in step 1) into a blender, mix according to the ratio of xanthan gum: water=3: 10000 to obtain a xanthan gum solution, add 70 parts of the xanthan gum solution to the blender and adjust to The substrate concentration is 50%. At a temperature of 45°C, use xanthan gum and broken-walled pine pollen to form a micro-reaction bed. Use alkali to adjust the pH of the solution to 9, and fully stir with a mixer for 60 minutes;
[0052] 3) Add trypsin 0.06% and bromelain 0.08% to the mixed solution obtained in step 2), the concentration of the compound enzyme is 40mg / g protein, and at a temperature of 60°C, put the mixed solution and the compound enzyme i...
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