Method for producing cosmetic base lotion by fermenting rice bud material
A technology for cosmetics and rice is applied in the field of making basic water for cosmetics by fermenting raw materials of rice sprouts, which can solve the problems of lack of literature reports and practical applications, immature technology and the like.
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Embodiment 1
[0040] Shake flask fermentation to prepare a small amount of cosmetic base water
[0041]Weigh 20g of rice seeds and fully soak them with normal temperature water, remove the seeds with low density floating on the water surface, wash them with running tap water and rub them for 10 minutes, drain the water, and use 70 mL of 2% sodium hypochlorite solution (containing 0.2% TritonX-100, The volume ratio of TritonX-100 to sodium hypochlorite (1:500) was soaked for 10 min, and washed 3 times with sterile water. Transfer the seeds into a sterilized 500 mL Erlenmeyer flask, add 50 mL of sterile water, seal it with 8 layers of gauze, and incubate at 25°C for 7 days, with a light intensity of 800 lux, 16 hours of light per day, and 8 hours of darkness.
[0042] Weigh 10 g of rice seedlings with a height ≥ 3 cm and endosperm into a mortar, add a little purified water and grind until the particle diameter is ≤ 1 mm, use purified water to make up to 200 mL, put the mixture in a 500 mL Erl...
Embodiment 2
[0045] Fermentation Tank Culture Preparation Cosmetic Basic Water Experiment
[0046] 1000 g of rice seeds were fully soaked with normal temperature water to remove the seeds with low density floating on the water surface, rinsed with running tap water and scrubbed for 10 minutes, drained, and washed with 3L of 2% sodium hypochlorite solution (containing Tween-80, in which The volume ratio of Tween and sodium hypochlorite is 1:1000), soaked for 15 min, and washed 3 times. Submerge the seeds in sterile water for 12 hours, rinse the seeds, spread 1000 g of seeds in 8-layer seedling trays, sprinkle water every 2 hours, rinse and change the water every 24 hours, under normal light conditions (1000 lux), After culturing at 25°C for 14 days, rice seedlings with a height ≥ 3 cm and endosperm can be obtained.
[0047] Rinse the rice seedlings and drain the water, weigh 1000g of rice seedlings with a height ≥ 3 cm and endosperm, crush them with a crusher for 10 min until the particle ...
Embodiment 3
[0050] Fermentation filtrate obtained from rice seeds as raw material as cosmetic base water
[0051] Weigh 20g of rice seeds and fully soak them with normal temperature water, remove the seeds with low density floating on the water surface, wash them with running tap water and rub them for 10 minutes, drain the water, and use 70 mL of 2% sodium hypochlorite solution (containing 0.2% TritonX-100, The volume ratio of TritonX-100 to sodium hypochlorite (1:500) was soaked for 10 min, and washed 3 times with sterile water. Weigh 10 g of sterile rice seeds and transfer them into a mortar, add a little purified water and grind until the particle diameter is ≤1 mm, dilute to 200 mL with purified water, put the mixture in a 500 mL Erlenmeyer flask, cover and seal with 8 layers of gauze, 100 Heating at ℃ for 10 min is the rice seed fermentation medium. After the culture medium was cooled to room temperature, 1 g of fresh weight yeast was introduced into the Erlenmeyer flask in an ultr...
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