Method for manufacturing soft algae specimen

A specimen making and software technology, applied in the direction of botany equipment and methods, application, plant preservation, etc., can solve the problems of not showing algae shape well, manual unfolding effect is not good, pressed specimens are easy to oxidize, etc., to achieve hardness Large size, good preservation, fast setting time

Pending Publication Date: 2020-06-26
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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AI-Extracted Technical Summary

Problems solved by technology

[0003] Existing methods for making seaweed specimens include a pressing method, which is to dry the algae by replacing the absorbent paper several times. Because the mollusc algae are small and delicate, in the drying process, the absorbent paper is replaced many times, which is easy to cause algae. The damage of the body, and the pressed specimen is easy to oxidize and deform
[0004] In previous studies, it was found that if the specimen preparation method of terrestrial plants was directly used for the preparation of mollusc specimens, the completed specimens could not show the morphology of the algae well.
Among them, pre-sample drying methods include air blowing, absorbent paper pressing, or desiccant, etc. Molluscum algae are small and delicate, and the methods of blowing air and absorbent paper pressing are easy to damage the algae and affect the shape of ...
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Abstract

The invention provides a method for manufacturing a soft algae specimen. The method comprises the following steps: step one, transferring algae of a specimen to be prepared to an expansion plate in water to enable the algae to be expanded on the expansion plate; step two, putting the expansion plate into a container, flatly laying a dried material/powder on the algae, and then carrying out dryingtreatment; step three, uniformly mixing a mixed glue solution prepared from epoxy resin and polyether amine D-230, performing defoaming at a temperature of not more than 40 DEG C, pouring the defoamedmixed glue solution into a mold, carrying out standing for 7-12 hours, taking down the algae dried in the step two from the expansion plate, adhering the algae onto the glue, covering the algae withthe defoamed mixed glue, and carrying out standing for 24-48 hours until the specimen is solidified; and step four, polishing the solidified algae specimen, coating UV glue, and carrying out UV lamp irradiation curing to complete the preparation. The specimen obtained by the method provided by the invention can better preserve the original form of algae.

Application Domain

Technology Topic

Algal speciesChemistry +4

Image

  • Method for manufacturing soft algae specimen
  • Method for manufacturing soft algae specimen
  • Method for manufacturing soft algae specimen

Examples

  • Experimental program(3)

Example Embodiment

[0027] Example 1 Preparation of Hymenophyllum specimens
[0028] 1) Extraction of the natural state of feather algae
[0029] Place the algae expansion plate ( figure 1 ) Place the algae in an incubator containing seawater, move the algae to the expansion board under water, adjust the angle to expand the algae, hold the two ends of the expansion board horizontally, and slowly lift the expansion board from underwater to On the water. The algae on the expansion board is flat and complete, close to the state of the algae under natural water.
[0030] 2) Drying of feather algae
[0031] SiO 2 Spread the dry powder flat to a thickness of 2cm at the bottom of the drying chamber, place the expansion plate with soft algae attached in it, and then slowly spread the dry powder onto the algae body, about 4cm thick, then close the drying chamber cover and place it for 10 minutes to The feather algae are dry. After drying is complete, remove the algae from the expansion board and proceed to the next steps.
[0032] 3) Glue fixation of feather algae
[0033] Choose a suitable size mold, mix the glue A (epoxy resin) and glue B (polyetheramine D-230) of the mixed glue until there is no wire drawing, and the mass ratio of glue A to glue B is 3:1; the mixing The solution is defoamed at a temperature of 35°C. The defoamed mixed glue is poured into the mold to the height of the liquid layer 0.5cm. After 12h, the fluidity of the glue will decrease. Gently remove the algae from the expansion plate. Rely on the viscosity of the glue to stick the algae to the glue, and then put the newly adjusted defoaming glue in the membrane, and let it stand for 48 hours until the specimen solidifies.
[0034] 4) Stripping and polishing
[0035] Demould the algae specimens, polish the uneven edges of the algae specimens after demolding, and then apply a layer of UV glue to UV light to cure.
[0036] The finished specimens of feather algae are stretched, natural, and bright in color, not much different from the original. The specimens are transparent and bright, three-dimensional, and the morphology of the algae can be observed from multiple directions ( figure 2 ).

Example Embodiment

[0037] Example 2. Preparation of Laver Specimen
[0038] 1) Extraction of the natural state of Porphyra yezoensis
[0039] Place the algae expansion board in the algae incubator filled with seawater, move the algae to the expansion board under water, adjust the angle to expand the algae, hold both ends of the expansion board horizontally and slowly move the expansion board from Lift underwater to the surface. The algae on the expansion board is flat and complete, close to the state of the algae under natural water.
[0040] 2) Drying of Porphyra yezoensis
[0041] SiO 2 Spread the dry powder flat to a thickness of 2cm at the bottom of the drying chamber, place the expansion plate with soft algae attached in it, and then slowly spread the dry powder onto the algae, about 4cm thick, and then close the drying chamber cover and leave it for 8 minutes to Nori dried.
[0042] 3) The glue fixation of Porphyra yezoensis
[0043] Choose a mold of appropriate size, mix the glue A (epoxy resin) and glue B (polyetheramine D-230) of the mixed glue until there is no wire drawing, and the mass ratio of glue A to glue B is 2.5:1; The solution was defoamed at a temperature of 39°C. The defoamed mixed glue was poured into the mold to the height of the liquid layer 0.5cm. After 12h, the fluidity of the glue was reduced. The algae was gently removed from the expansion plate. Rely on the viscosity of the glue to stick the algae to the glue, and then place the newly adjusted defoamed glue in the membrane, and let it stand for 36 hours until the specimen solidifies.
[0044] 4) Stripping and polishing
[0045] Demould the algae specimens, polish the uneven edges of the algae specimens after demolding, and then apply a layer of UV glue to UV light to cure.
[0046] The finished laver specimen has a stretched shape, natural and bright in color, not much different from the original. The specimen is transparent and bright, three-dimensional, and the shape of the algae can be observed from multiple directions.

Example Embodiment

[0047] Example 3 Preparation of a specimen
[0048] The main branch of the algae is not soft, but the branches are more, and the leaflets are more dense and dense. This method can also better preserve the morphology of the algae.
[0049] 1) Extraction of the natural state of the
[0050] Place the algae expansion board in the algae incubator filled with seawater, move the algae to the expansion board under water, adjust the angle to expand the algae, hold both ends of the expansion board horizontally and slowly move the expansion board from Lift underwater to the surface. The algae on the expansion board is flat and complete, close to the state of the algae under natural water.
[0051] 2) Drying of Drosophila
[0052] SiO 2 Spread the dry powder flat to a thickness of 2cm at the bottom of the drying chamber, place the expansion plate with soft algae attached in it, and then slowly spread the dry powder onto the algae, about 4cm thick, and then close the drying chamber cover and leave it for 8 minutes to The Drosophila dry.
[0053] 3) Glue fixation of Algae
[0054] Choose a suitable size mold, mix the glue A (epoxy resin) and glue B (polyetheramine D-230) of the mixed glue until there is no wire drawing, and the mass ratio of glue A to glue B is 3:1; the mixing The solution was defoamed at a temperature of 39°C. The defoamed mixed glue was poured into the mold to the height of the liquid layer 0.5cm. After 12h, the fluidity of the glue was reduced. The algae was gently removed from the expansion plate. Rely on the viscosity of the glue to stick the algae to the glue, and then place the newly adjusted defoamed glue in the membrane, and let it stand for 36 hours until the specimen solidifies.
[0055] 4) Stripping and polishing
[0056] Demould the algae specimen, and polish the edges of the algae specimen that are not round after demoulding. After polishing the specimen like image 3 Shown.
[0057] Then apply a layer of UV glue and cure by UV lamp irradiation. Specimens after applying UV glue such as Figure 4 Shown.
[0058] The finished specimens of Asparagus algae are stretched, bright and natural in color, with little difference from the original state. The specimens are transparent and bright, three-dimensional, and the morphology of the algae can be observed from multiple directions.
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