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An immunosensor for diagnosing early lung cancer tumor markers and its preparation method and application

A technology of tumor markers and immunosensors, applied in the field of diagnosis of early lung cancer tumor markers immunosensors and its preparation

Active Publication Date: 2020-10-20
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical difficulty of electrochemical immunosensors lies in how to improve the magnitude of the current and its detection sensitivity and selectivity.

Method used

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  • An immunosensor for diagnosing early lung cancer tumor markers and its preparation method and application
  • An immunosensor for diagnosing early lung cancer tumor markers and its preparation method and application
  • An immunosensor for diagnosing early lung cancer tumor markers and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] An immunosensor for diagnosing early lung cancer tumor markers. The lung cancer tumor marker immunosensor includes: a working electrode, a reference electrode and a counter electrode. The base electrode of the working electrode is a glassy carbon electrode, and its surface is sequentially modified by two Manganese oxide / hollow nano-gold complex, squamous cell carcinoma antibody, bovine serum albumin (BSA), the reference electrode is a saturated calomel electrode, and the counter electrode is a platinum wire electrode.

[0043] A method for preparing an immunosensor for diagnosing early lung cancer tumor markers, comprising the following steps:

[0044] S1. Preparation of manganese dioxide / hollow gold nanosphere composite:

[0045] Ultrasonic dispersion of 500mg hollow gold nanospheres in 8% polyethylene glycol and 0.1mol / L cetyltrimethylammonium bromide 30mL aqueous solution, adding 0.158g KMnO 4 The solution was reacted at 90°C for 3 hours by liquid-phase co-precipitati...

Embodiment 2-6

[0064] A method for preparing a lung cancer tumor marker immunosensor, comprising the following steps: selecting five lung cancer tumor markers, CEA, CA-242, CA-125, NSE or TPA, and constructing a lung cancer tumor marker according to the steps described in Example 1 Immunosensors were used for detection, and the detection technical indicators of lung cancer tumor markers are shown in Table 1.

[0065] Table 1 Detection technical indicators of tumor markers in lung cancer

[0066] Tumor markers Linear range (ng / mL) Detection limit ((pg / mL)) CEA 0.01～10 2.1 CA-242 0.01～20 10.2 CA-125 0.01～10 12.5 NSE 0.01～20 3.8 TPA 0.01～20 2.8

Embodiment 7

[0068] This example provides an electrochemical immunosensor prepared in Example 1 for the detection of lung cancer tumor markers in human serum samples, the method is as follows:

[0069] Accurately pipette human serum samples, add a certain mass concentration of lung cancer tumor marker antigen standard solution, use the human serum without lung cancer tumor marker antigen as a blank, carry out the standard addition recovery experiment, and detect according to the steps of Example 1. The recovery rates of lung cancer tumor markers in the samples are shown in Table 2.

[0070] Table 2 Detection results of lung cancer tumor markers in human serum

[0071]

[0072] As can be seen from the test results in Table 2, the relative standard deviation (RSD) of the results is less than 2.5%, and the average recovery rate is 98.8~102.2%, showing that the present invention can be used for the detection of lung cancer tumor markers in human serum, and the present invention provides Th...

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Abstract

The invention discloses a tumor marker immunosensor for diagnosing early lung cancer as well as a preparation method and application of the tumor marker immunosensor. Hollow gold nanospheres preparedby taking an ionic liquid as a ligand are used as a matrix; potassium permanganate is used as manganese source, hexadecyl trimethyl ammonium bromide and polyethylene glycol are used as surfactants; amanganese dioxide / hollow nano gold ball compound by adopting a liquid-phase coprecipitation method; an electrochemical immunosensor for diagnosing the early lung cancer tumor marker is prepared by taking the aptamer as an electrode modifier; the hollow gold nanospheres and the nano petal-shaped manganese dioxide jointly modify the electrode to synergistically increase the specific surface area ofthe electrode and increase the antibody adsorption capacity, so that the sensitivity and selectivity of the electrochemical immunosensor are remarkably improved, rapid, high-sensitivity and high-selectivity detection of a sample is realized, and the immunosensor has a wide market development prospect. The lung cancer tumor marker immunosensor provided by the invention is simple to prepare and convenient to operate.

Description

technical field [0001] The invention relates to the technical field of novel nano-functional materials and biosensors, in particular to an immunosensor for diagnosing early lung cancer tumor markers and its preparation method and application. Background technique [0002] Lung cancer is one of the most threatening malignant tumors to human health and life, and its morbidity and mortality are increasing the fastest. According to reports, the incidence of lung cancer has increased significantly in the past fifty years. Among male malignant tumor patients, the incidence and mortality of lung cancer accounted for the first place, and among female malignant tumor patients, the incidence and mortality both occupied the second place. . Therefore, early diagnosis and early treatment of lung cancer are the key to improving the survival rate of lung cancer patients. The detection of lung cancer tumor markers can reflect the biological characteristics of lung cancer, which is of grea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N27/48G01N27/327G01N27/32G01N27/30
CPCG01N27/301G01N27/308G01N27/32G01N27/3277G01N27/3278G01N27/48G01N33/57423G01N33/57484
Inventor 刘宁吕静郭婧魏雪
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV