Compound composition of phenazino-1-carboxylic acid and biphenyl triadimenol and application of compound composition
A compound composition and technology of bifentriazole, which can be applied in application, biocide, animal repellent, etc., can solve problems such as endocrine system damage
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Embodiment 1
[0018] Indoor toxicity test and compound ratio screening
[0019] 1.1 Drugs to be tested
[0020] 95% Shenzimycin technical, product of Shanghai Nongle Biological Products Co., Ltd.
[0021] 97% bifentriazole technical product, product of Jiangsu Jianpai Agrochemical Co., Ltd.
[0022] 1.2 Tested strains
[0023] The test targets are peanut brown spot fungus (Cercospora arachidicola), black spot fungus (Cercosporapersonata), net spot fungus (Phoma arachidicola).
[0024] The samples of pathogen strains were isolated from diseased leaves in the field, and the first-generation strains were cultivated and purified, and stored in a refrigerator at 4°C for future use.
[0025] 1.3 Instruments and equipment
[0026] There are mainly electronic balances, ultra-clean workbenches, biological incubators, petri dishes, micropipettes, etc.
[0027] 1.4 Experimental method
[0028] The Mycelium growth rate test recommended by the FAO was used.
[0029] 1.4.1 Drug compounding
[003...
Embodiment 2
[0065] field plot experiment
[0066] According to the indoor compound ratio screening experiment, the optimum ratio of Shenzimycin:Bifentriazole = 1:20 was selected, and we prepared a 21% Shenzimycin·Bifentriazole compound composition. According to the "Pesticide Field Efficacy Test Guidelines (2)" edited by the Pesticide Control Institute of the Ministry of Agriculture, the field efficacy test of 21% Shenzimycin·Bifentriazole compound composition on peanut leaf spot was designed.
[0067] 2.1 Drugs to be tested
[0068] 1% Shenzimycin Suspension Concentrate 100mL / 667m 2 , Shanghai Nongle Biological Products Co., Ltd.
[0069] 25% bifentriazole wettable powder 66g / 667m 2 , Jiangsu Jianpai Agrochemical Co., Ltd.;
[0070] 21% Shenzimycin Bifentriazole 60, 80, 100mL / 667m 2 , prepared by Yantai Academy of Agricultural Sciences.
[0071] 2.2 Control drug
[0072] 500g / L Carbendazim Suspension Concentrate 100m / 667m 2 , Products of Jiangsu Longdeng Chemical Co., Ltd.
[00...
Embodiment 3
[0091] safety test
[0092] The contents of shenazinamycin and bifontriazole in the compound composition are less than those of commercially available products, so its toxicity to humans and animals and the safety of crops meet the requirements. The results of the toxicity test of the accredited institution show that the compound fungicide belongs to the slightly toxic pesticide:
[0093] Rat acute oral toxicity LD50♂>5000mg / kg,♀>5000mg / kg;
[0094] Rat acute dermal toxicity LD50♂>20000mg / kg,♀>20000mg / kg.
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