Melon and fruit antiseptic and fresh-keeping agent, preparation method thereof and melon and fruit storage method
A preservative, melon and fruit technology, applied in the direction of protecting fruits/vegetables with a protective layer, preserving fruits/vegetables and food ingredients by freezing/refrigeration, etc.
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Embodiment 1
[0039] Such as Figure 5 As shown, the preparation method of the kiwi fruit antiseptic and fresh-keeping agent of the present invention includes the following steps:
[0040] 1. Target the pathogens that affect the rot of kiwifruit.
[0041] a. Take Hongyang kiwi as the test fruit, the same below. High-throughput sequencing (Illumina HiSeq) was used to understand the main dominant pathogen species of kiwi fruit soft rot.
[0042] b. Isolation of major dominant pathogens. Take the kiwi fruit pulp (0.2cm away from the lesion), rinse with sterile water 3 times, soak in 70% ethanol for 8 seconds, then rinse with plenty of sterile water, and blot the sterile water with sterile paper Afterwards, a small amount of tissue was inoculated on a PDA plate, cultured in a 27°C incubator in the dark, 4 days after culture.
[0043] b. Purification of pathogenic bacteria. Pick the dominant colonies in the above-mentioned plate and inoculate them on another new plate. Cultivate for 4 days in a 27°C...
Embodiment 2
[0079] Such as Figure 5 As shown, the preparation method of the kiwi fruit antiseptic and fresh-keeping agent of the present invention includes the following steps:
[0080] 1. Target the pathogens that affect the rot of kiwifruit.
[0081] a. Take the jade kiwi fruit as the test fruit, the same below. High-throughput sequencing (Illumina HiSeq) was used to understand the main dominant pathogen species of kiwi fruit soft rot.
[0082] b. Isolation of major dominant pathogens. Take the kiwi fruit pulp (0.25cm away from the diseased spot), rinse with sterile water 3 times, soak in 75% ethanol for 8 seconds, rinse with plenty of sterile water, and blot the sterile water with sterile paper Afterwards, a small amount of tissue was inoculated on a PDA plate, cultured in a 27°C incubator in the dark, 4 days after culture.
[0083] b. Purification of pathogenic bacteria. Pick the dominant colonies in the above-mentioned plate and inoculate them on another new plate. Incubate for 4 days i...
Embodiment 3
[0110] The preparation method of melon and fruit antiseptic and fresh-keeping agent includes the following steps:
[0111] 1. Configuration of antiseptic and fresh-keeping agent
[0112] a. According to the results of the previous collaborative antibacterial test, and further optimization. Weigh the above-mentioned reagents according to the formula. Among them, the oil-tea meal extract is both a sterilization and a mixed solvent. The emulsifying thickener is a mixture of chitosan and sodium alginate. Scutellaria baicalensis extract 0.05%, clove oil 0.15%, cinnamon oil 0.05%, saponin thorn extract 0.06%, patchouli oil 0.17%, calamus oil 0.17%, camellia meal extract 0.5%, chitosan 0.30%, chlorinated Calcium 1.5%, sodium alginate 7%, carrageenan 2.5%, citric acid 0.3%; the balance is water, and the total amount of raw materials is 100%.
[0113] b. First, mix the extract of scutellaria baicalensis Georgi, clove oil, cinnamon oil, saponaria saponaria extract, patchouli oil, and calamus...
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