Digital chip and method for nucleic acid amplification

Abstract

The invention provides a digital chip and method for nucleic acid amplification. The digital chip for nucleic acid amplification includes a digital detection array reaction chamber, a main sample inlet and a main sample outlet, wherein the digital detection array reaction chamber includes multiple main channels and multiple micro-units, each micro-unit communicates with the main sample inlet and the main sample outlet through one main channel, and each micro-unit includes a micro-unit reaction chamber; and the inlet and outlet of each micro-unit reaction chamber separately communicate with thecorresponding main channel, and the width of the section, after the joint of the inlet of each micro-unit reaction chamber with the corresponding main channel, of the corresponding main channel is reduced, so that when a water phase in the corresponding main channel passes through the section, the water phase is introduced into each micro-unit reaction chamber, and the flow direction of an oil phase is unchanged. The fluid behavior of a liquid in the hydrophobic channels is controlled through an oil-water interfacial tension, therefore, a PCR reagent is stored in the designed micro-reaction chamber, and is sealed with oil, and the digital chip can be applied to nucleic acid amplification detecting experiments efficiently and conveniently; and the problems of a complex chip technology, high requirements of a manufacture process and inconvenient operation can be solved in the field of dPCR.

Description

technical field [0001] The invention belongs to the field of medical inspection and analysis, and in particular relates to a digital microfluidic chip and method applicable to nucleic acid amplification. Background technique [0002] Nucleic acids, including DNA (deoxyribonucleic acid) and RNA (ribonucleic acid), are the most important biological macromolecules found in organisms carrying genetic information, and have been widely used as important biomarkers for detecting various diseases. In diagnosing an inherited or acquired disease, nucleic acid testing is often performed to determine the concentration of the target (eg, the number of copies of the target nucleotide sequence). However, the concentration of target nucleic acid in clinical samples (such as blood) is usually low, and usually most existing detection methods cannot detect nucleic acid samples with very low concentrations, so the target molecule needs to be amplified. Polymerase Chain Reaction (Polymerase Cha...

AI Technical Summary

Problems solved by technology

In order to solve the problems of complex chip technology, high preparation process requirements and inconvenient operation in the current dPCR field

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