Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescent probe for detecting total mercury and preparation method, kit and detection method thereof

A fluorescent probe and detection method technology, applied in the field of mercury ion detection, can solve problems such as weak binding ability and reactivity, poor coordination ability and thiophilicity, and difficulty in simultaneous identification, achieving low cost, good solubility, and detection The effect of high sensitivity

Active Publication Date: 2020-07-17
DATANG ENVIRONMENT IND GRP
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The detection of mercury by fluorescent probe method is low in detection cost, and can avoid cumbersome pretreatment process and complicated instrument operation. Mercury has weak binding ability and reactivity to probes that can recognize mercury ions, which makes it difficult for the same fluorescent probe to simultaneously recognize methylmercury and mercury ions in the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent probe for detecting total mercury and preparation method, kit and detection method thereof
  • Fluorescent probe for detecting total mercury and preparation method, kit and detection method thereof
  • Fluorescent probe for detecting total mercury and preparation method, kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] S11. Add 4.0mmol of 4-bromo-1,8-naphthalic anhydride powder and 1.50mmol of diethylenetriamine liquid to 100mL of ethanol successively, heat and reflux in a two-necked round bottom flask for 7 hours, and gradually generate the compound , after cooling, the solution was filtered and fully washed with ethanol to wash off excess raw materials to obtain solid product A with a yield of up to 82%.

[0065] S12. Mix and dissolve 4.0 mmol of tetraethylene glycol monomethyl ether liquid and 6.0 mmol of sodium in 50 mL of toluene solution, and under the protection of nitrogen, fully stir and react at room temperature for 3 hours. During the reaction, the solution gradually turns light yellow, and the reaction is over. After obtaining sodium alkoxide and two unreacted raw materials, the excess metallic sodium is filtered to obtain sodium alkoxide solution B.

[0066] S13. Mix the solid product A obtained in step S11 with 0.9 mmol of the sodium alkoxide solution B obtained in step ...

Embodiment 2

[0069] S21. Add 4.3mmol of 4-bromo-1,8-naphthalene dicarboxylic anhydride powder and 1.80mmol of diethylenetriamine liquid to 100mL of ethanol successively, heat and reflux in a two-necked round-bottomed flask for 8 hours, and gradually generate the compound , after cooling, the solution was filtered and fully washed with ethanol to wash away excess raw materials to obtain solid product A with a yield of up to 87%.

[0070] S22. Mix and dissolve 4.5 mmol of tetraethylene glycol monomethyl ether liquid and 6.25 mmol of sodium in 50 mL of toluene solution, and under nitrogen protection, fully stir and react at room temperature for 4 hours. During the reaction, the solution gradually turns light yellow, and the reaction is over. After obtaining sodium alkoxide and two unreacted raw materials, the excess metallic sodium is filtered to obtain sodium alkoxide solution B.

[0071]S23. Mix the solid product A obtained in step S21 with 0.96 mmol of the sodium alkoxide solution B obtain...

Embodiment 3

[0074] S31. Add 4.6 mmol of 4-bromo-1,8-naphthalene dicarboxylic anhydride powder and 2.1 mmol of diethylenetriamine liquid to 100 mL of ethanol successively, heat and reflux in a two-necked round-bottomed flask for 9 hours, and gradually generate a compound , after cooling, the solution was filtered and fully washed with ethanol to wash off excess raw materials to obtain solid product A with a yield of up to 89%.

[0075] S32. Mix and dissolve 4.5 mmol of tetraethylene glycol monomethyl ether liquid and 6.25 mmol of sodium in 50 mL of toluene solution, and under the protection of nitrogen, fully stir and react at room temperature for 5 hours. During the reaction, the solution gradually becomes light yellow, and the reaction is completed. After obtaining sodium alkoxide and two unreacted raw materials, the excess metallic sodium is filtered to obtain sodium alkoxide solution B.

[0076] S33. Mix the solid product A obtained in step S31 with 1.0 mmol of the sodium alkoxide solu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of mercury ion detection, in particular to a fluorescent probe, a fluorescent probe kit and a detection method for detecting total mercury. According to the fluorescent probe disclosed by the invention, naphthalimide is used as a fluorescent group, a pyridine group is used as a binding site, and when the probe independently exists in a solution, excitation energy can be subjected to non-radiative annihilation due to free rotation of the 1, 8-naphthalimide fluorescent group, so that the probe cannot be excited to emit strong fluorescence. However, after mercury ions or methyl mercury in a water environment is subjected to coordination binding with the probe, the rotation of the 1, 8-naphthalimide group is limited, so that the probe can be excitedto emit strong fluorescence. According to the fluorescent probe molecule disclosed by the invention, nitrogen atoms on pyridine and carbonyl on naphthalimide are used as binding sites of mercury, themercury ions and the methyl mercury can be combined with the fluorescent probe to generate an RIR effect while the high selective specificity of the mercury is ensured, so that fluorescence is emitted under the excitation of ultraviolet light, and the fluorescent probe has the advantage of high detection sensitivity.

Description

technical field [0001] The invention relates to the technical field of mercury ion detection, in particular to a fluorescent probe for detecting total mercury, a fluorescent probe kit and a detection method. Background technique [0002] Heavy metal pollution has become a problem that cannot be ignored in our country. Among them, mercury is considered to be one of the most ubiquitous toxic metals in the environment, which can have harmful or even fatal effects on animals and plants. The "total mercury" that is ubiquitous in nature and causes pollution mainly exists in the form of two mercury compounds, mercury ions and methylmercury. Its harm to humans and animals is mainly concentrated in the central nervous system, digestive system and internal organs. A few milligrams entering the body may cause serious harm to the liver, kidneys and nervous system. In addition, it is also harmful to the respiratory system, blood, skin and eyes. Certain effects; its effects on plants can...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D401/14C09K11/06G01N21/64
CPCC07D401/14C09K11/06C09K2211/1029G01N21/6402G01N21/6428
Inventor 杨言刘海洋白玉勇谷小兵李飞麻晓越荆亚超高阳彭思伟
Owner DATANG ENVIRONMENT IND GRP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com