Chitosan nano-selenium hydrosol with immune enhancement effect and preparation, preservation and application

A technology of chitosan nano and immune enhancement, applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, applications, etc., can solve the problem of lack of immune enhancers, to avoid adverse effects, preparation steps Simple, simple and feasible process

Active Publication Date: 2020-07-21
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problem that the existing aquatic disease prevention and control in the aquaculture industry lacks the problem of effe...

Method used

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  • Chitosan nano-selenium hydrosol with immune enhancement effect and preparation, preservation and application
  • Chitosan nano-selenium hydrosol with immune enhancement effect and preparation, preservation and application
  • Chitosan nano-selenium hydrosol with immune enhancement effect and preparation, preservation and application

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preparation example Construction

[0044] In one embodiment, the preparation method of the chitosan nano-selenium hydrosol with immunoenhancing effect may further comprise the steps:

[0045] Step S01, adding a solution containing selenium ions and / or selenium ions to the functionalized chitosan aqueous solution to obtain a mixed solution;

[0046] Step S02. Add a reducing agent solution dropwise to the mixed solution obtained in step S01, mix to make a reduction reaction occur, and obtain a reaction product;

[0047] Step S03. The reaction product obtained in step S02 is subjected to constant volume and dialysis treatment to obtain the chitosan nano-selenium hydrosol with immunoenhancing effect.

[0048] In any embodiment, chitosan is dissolved by organic acid solution to obtain functionalized chitosan, and the water solubility of functionalized chitosan becomes better than chitosan, in the form of functionalized chitosan aqueous solution exist.

[0049] Above-mentioned organic acid solution can be any one i...

Embodiment 1

[0070] A preparation method of chitosan nano-selenium hydrosol with immune enhancing effect, comprising the following steps:

[0071] (1). At 20°C, under 101.325kPa, take 1.0mL, 2.0mL, and 10mL of functionalized chitosan aqueous solutions with a mass concentration of 3000mg / L and add them into 25mL volumetric flasks (volume bottle No. 1#, 2#, 3#), wherein the functionalized chitosan is chitosan treated with citric acid, and the average molecular weight of the functionalized chitosan is 200000Da;

[0072](2). Add 0.25mL, 1.0mL, and 2.5mL of sodium selenite solutions with a concentration of 60mmol / L to the 25mL volumetric flasks numbered 1#, 2#, and 3# above, and shake them gently to make them Mix well to obtain a mixed solution;

[0073] (3). Add dropwise 0.19mL, 1.0mL, 3.29mL of vitamin C solutions with a concentration of 320mmol / L to the mixed solution of (2), shake gently while adding, add water to make up to 25mL , Stand still, wait for the red color to no longer deepen, ...

Embodiment 2

[0088] A kind of preparation method of chitosan nano-selenium hydrosol with immunoenhancing effect and as the application of aquatic product immunity enhancer, its preparation method comprises the following steps:

[0089] (1). At 20°C, under 101.325kPa, take 2.0mL of a functionalized chitosan aqueous solution with a mass concentration of 3000mg / L, add it to a 25mL volumetric flask equipped with 5mL double-distilled water, and the functionalized chitosan The sugar is to mix chitosan with citric acid to improve the water solubility of chitosan, and the average molecular weight of functionalized chitosan is 200000Da;

[0090] (2). Add 1.0mL of sodium selenite solution with a concentration of 60mmol / L to the above-mentioned 25mL volumetric flask, shake it up gently, and make it fully mixed to obtain a mixed solution;

[0091] (3). Add 1.0mL of vitamin C solution with a concentration of 320mmol / L to the mixed solution of (2) dropwise, and shake gently while adding. No further dee...

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Abstract

The invention relates to the technical field of nano-selenium hydrosols, in particular to a chitosan nano-selenium hydrosol with an immune enhancement effect and preparation, preservation and application. The chitosan nano-selenium hydrosol with the immune enhancement effect comprises the following components in concentration: 0.5mmol/L-5.0mmol/L of nano-selenium and 100.0mg/L-1000.0mg/L of functionalized chitosan. The chitosan nano-selenium hydrosol with the immune enhancement effect has immune activity, and can increase the selenium content in muscle tissue of aquatic organisms including fishes and the like; and when the chitosan nano-selenium hydrosol is added into aquatic organism feed and serves as an immune enhancer for aquatic organisms, a more effective scheme can be provided for disease control of aquaculture.

Description

technical field [0001] The invention relates to the technical field of nano-selenium hydrosol, in particular to a chitosan nano-selenium hydrosol with immune enhancement effect and its preparation, preservation and application. Background technique [0002] China is the world's largest producer of aquatic products, with an annual direct economic value of about 900 billion yuan. However, frequent outbreaks of aquaculture diseases in recent years have affected the health of aquatic products and caused huge economic losses. In 2012 alone, the economic loss caused by aquaculture diseases in my country reached 3.6 billion yuan (China Fishery Statistical Yearbook, 2013). Aquaculture diseases caused by common aquaculture pathogens such as Vibrio anguillarum, Aeromonas hydrophila and Yersinia ruckeri of the red mouth disease, etc., give the aquaculture industry annual cause huge economic losses. In order to prevent and treat aquatic diseases and improve the immune function of aqu...

Claims

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Application Information

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IPC IPC(8): A61K33/04A61K9/06A61K47/36A61P37/04A23K50/80A23K20/163A23K20/20A61K31/722
CPCA61K33/04A61K31/722A61K9/06A61K47/36A61P37/04A23K50/80A23K20/163A23K20/30A61K2300/00
Inventor 夏凡郭颖轩黄家兴陆家谦张兆滔
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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