Method for determining four diester aconitum alkaloids in blood

A technology of diester type and aconitum, which is applied in the field of determination of four diester type aconite alkaloids in blood, can solve the problems of inability to real-time monitoring, long detection time, and many interfering substances, and achieves elimination of matrix effect, Simple operation and efficient sample handling

Inactive Publication Date: 2020-07-21
NINGBO MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the problems of many interfering substances, long detection time, low accuracy and inability to monitor in real time in the existing detection method of aconitum alkaloids, the present invention provides a fast and accurate method, the result is objective and easy to analyze, and real-time monitoring can be realized Method for Determination of 4 Kinds of Diester Type Aconitum Alkaloids in Blood

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  • Method for determining four diester aconitum alkaloids in blood
  • Method for determining four diester aconitum alkaloids in blood
  • Method for determining four diester aconitum alkaloids in blood

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Embodiment 1

[0047] A method for measuring 4 kinds of diester aconitine alkaloids in blood, comprising the following steps:

[0048] (1) Take 2ml of whole blood sample in a centrifuge tube, centrifuge for 5min under a centrifugal force of 3000g, draw the supernatant in another test tube to obtain a plasma sample, add 5 μl of formic acid aqueous solution with a volume ratio of 10% to the plasma sample, Store in a refrigerator at 4°C for later use;

[0049] (2) Aspirate 200 μl of the plasma sample treated in step (1), add 1 mL of 0.5% formic acid / acetonitrile solution as an extraction solvent, centrifuge at a centrifugal force of 3000 g for 2 min, and absorb the supernatant to obtain an extract;

[0050] (3) Transfer 0.5mL of the extract to another 2mL polypropylene centrifuge tube, then add 10.0mg of magnetic graphene-based polyamide (PAA@Mag-G), vortex for 5min, and centrifuge at 3000g for 5min , absorb the supernatant to obtain the purified solution;

[0051] (4) Filter the purified sol...

Embodiment 2

[0074] A method for measuring 4 kinds of diester aconitine alkaloids in blood, comprising the following steps:

[0075] (1) Take 2ml of whole blood sample in a centrifuge tube, centrifuge at a centrifugal force of 3000g for 5min, draw the supernatant in another test tube to obtain a plasma sample, add 6 μl of formic acid aqueous solution with a volume fraction of 10% in the plasma sample, Store in a refrigerator at 3°C ​​for later use;

[0076] (2) Aspirate 200 μl of the plasma sample treated in step (1), add 1 mL of 0.5% formic acid / acetonitrile solution as an extraction solvent, centrifuge at a centrifugal force of 3000 g for 2 min, and absorb the supernatant to obtain an extract;

[0077] (3) Transfer 0.5mL of the extract to another 2mL polypropylene centrifuge tube, then add 10.0mg of magnetic graphene-based polyamide (PAA@Mag-G), vortex for 5min, and centrifuge at 3000g for 5min , absorb the supernatant to obtain the purified solution;

[0078] (4) Filter the purified sol...

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Abstract

The invention relates to the technical field of drug detection, and provides a method for determining four diester aconitum alkaloids in blood in order to solve the problems of many interferents, longdetection time and low accuracy of an aconitum alkaloid detection method. The method comprises the following steps: (1) adding a stabilizer into a plasma sample, and storing the plasma sample at lowtemperature for later use; (2) adding an extraction solvent for extraction to obtain an extracting solution; (3) adding a magnetic graphene-based adsorbent for purification treatment to obtain a purified solution; and (4) filtering the purified liquid, and performing HPLC-MS/MS on-machine detection and analysis. According to the invention, the method for detecting four diester aconitum alkaloids in a blood sample is established through pretreatment of a blood sample and optimization of detection conditions, the method is rapid and accurate, the result is objective and easy to analyze, the content change of the four diester aconitum alkaloids in a poisoned patient can be monitored in real time, and a scientific basis can be provided for clinical diagnosis and treatment of aconitum alkaloidpoisoning.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for measuring four diester-type aconitum alkaloids in blood. Background technique [0002] Aconitum plants have the effects of expelling wind and dampness, warming meridians and relieving pain. They can also be used for wind-cold and arthralgia, joint pain, cold pain in trusted subordinates, cold colic pain, anesthesia pain, etc., and are widely used in clinical treatment. The main components of this type of traditional Chinese medicine are diester-type aconitum alkaloids, accounting for about 7-10% of the total weight. Diester-type aconitum alkaloids are not only the toxic components of traditional Chinese medicines of the genus Aconitum, but also the material basis of their efficacy. Since there is no unified processing method for aconitum traditional Chinese medicine, poisoning or death may occur from time to time due to excessive dosage, insufficient decoctio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08G01N30/14G01N30/72C08K3/04C08G73/02C08L79/02
CPCG01N30/02G01N30/06G01N30/08G01N30/14G01N30/72C08K3/042C08G73/028G01N2030/062C08K2201/01
Inventor 金米聪何仟陈晓红周健潘胜东蔡美强张昱
Owner NINGBO MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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