A kind of recombinant vector and its construction method and application

A technology for recombining vectors and constructing methods, which is applied in the direction of vectors, applications, nucleic acid vectors, etc., to achieve the effect of easy operation, simple operation and accurate results
CN111440823BActive Publication Date: 2020-12-01INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
Publication Date
2020-12-01

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Abstract

The invention provides a recombinant vector and its construction method and application, which belong to the technical field of insect genetic engineering. The recombinant vector includes a dual-luciferase reporter gene carrier and the nucleotide sequence of the 3'UTR region of the brown planthopper Ccdc124 gene; The method for constructing the recombinant vector comprises the following steps: 1) using the cDNA of the brown planthopper as a template, performing PCR amplification to obtain the 3'UTR region nucleotide sequence of the Ccdc124 gene of the brown planthopper; 2) converting the 3'UTR of the Ccdc124 gene of the brown planthopper to The nucleotide sequence of the region was recombined into the dual luciferase reporter gene vector to obtain a recombinant vector. The recombinant vector provided by the invention can measure the expression activity of the Ccdc124 gene of the brown planthopper, be used for screening the microRNA that regulates the Ccdc124 gene of the brown planthopper, and can also study the regulation mechanism of the predicted microRNA regulating the Ccdc124 gene.
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Description

technical field

[0001] The invention belongs to the technical field of insect genetic engineering, and in particular relates to a recombinant vector and its construction method and application. Background technique

[0002] Brown planthopper (Nilaparvata lugens) ), belonging to the family Delphacidae in the order Homoptera. Mainly distributed in East Asia, Southeast Asia, the South Asian subcontinent, northern Australia and the South Pacific Islands. Rice is the main host of BPH, and some wild rice and barnyardgrass in the field may also become host plants. Generally speaking, under natural conditions, BPH cannot repeat generations on other plants. The brown planthopper likes a hidden and humid environment, and adults and nymphs generally gather in the lower part of the rice bushes. When brown planthoppers occur in large numbers, thousands of insects often gather at the base of each rice pocket. In severe cases, the lower part of the rice plants will turn black, paralyze...

Claims

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