Tetranychus cinnabarinus prevention and treatment compound extracted from peanut branches and leaves and application
A technology for cinnabar spider mite and peanut branches and leaves is applied in the field of botanical pesticide agricultural acaricides to achieve the effects of reducing economic losses, reducing the degree of harm and avoiding harm
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Embodiment 1
[0038] The active compounds extracted from the crude extracts of peanut branches and leaves were applied to the control test of Tetranychus cinnabarinus:
[0039] Take by weighing 20g of crude extract of peanut branches and leaves, use silica gel as stationary phase, and use petroleum ether-ethyl acetate (volume ratio is 1:0-0:1) and ethyl acetate-methanol (volume ratio is 1:0- 0:1) to obtain a variety of crude extracts; use medium pressure column chromatography, use C18 as the stationary phase, and the mobile phase selects methanol-water (volume ratio is 1:0-0:1) or chloroform-methanol (volume ratio is 1:0-0:1) 1:0-0:1) to separate and purify the crude extract to obtain a purified product; using high performance liquid chromatography, the active compound was measured to be palmitic acid by comparison with the standard product. The control test of Tetranychus cinnabarinus was carried out with the compounds obtained by the above separation and purification. The contact activit...
Embodiment 2
[0041] The active compounds extracted from the crude extracts of peanut branches and leaves were applied to the control test of Tetranychus cinnabarinus:
[0042] Take by weighing 20g of crude extract of peanut branches and leaves, use silica gel as stationary phase, and use petroleum ether-ethyl acetate (volume ratio is 1:0-0:1) and ethyl acetate-methanol (volume ratio is 1:0- 0:1) to obtain a variety of crude extracts; use medium pressure column chromatography, use C18 as the stationary phase, and the mobile phase selects methanol-water (volume ratio is 1:0-0:1) or chloroform-methanol (volume ratio is 1:0-0:1) 1:0-0:1) to separate and purify the crude extract to obtain a purified product; using high performance liquid chromatography, the active compound was measured as isovanillic acid by comparing with the standard product. The control test of Tetranychus cinnabarinus was carried out with the compounds obtained by the above separation and purification. The contact activity...
Embodiment 3
[0044] The active compounds extracted from the crude extracts of peanut branches and leaves were applied to the control test of Tetranychus cinnabarinus:
[0045] Take by weighing 20g of crude extract of peanut branches and leaves, use silica gel as stationary phase, and use petroleum ether-ethyl acetate (volume ratio is 1:0-0:1) and ethyl acetate-methanol (volume ratio is 1:0- 0:1) to obtain a variety of crude extracts; use medium pressure column chromatography, use C18 as the stationary phase, and the mobile phase selects methanol-water (volume ratio is 1:0-0:1) or chloroform-methanol (volume ratio is 1:0-0:1) 1:0-0:1) to separate and purify the crude extract to obtain a purified product; using high performance liquid chromatography, the active compound was determined to be β-sitosterol by comparing with the standard product. The control test of Tetranychus cinnabarinus was carried out with the compounds obtained by the above separation and purification. The contact activit...
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