High-flux cell sorting and enriching device and using method thereof

Abstract

Aiming at the technical defects of low target cell screening flux, large damage, easy loss and pollution of recovered cells and the like in the existing cell research technology, the invention provides a high-flux cell sorting and enriching device and a using method thereof. The device has the following advantages: 1) cell enrichment, recovery, capture and culture are integrated in a closed mode,so the problems of inter-link cell loss and pollution are solved; 2) on a cell separation module, by combining an inclined cell filter membrane and a fluid channel, the target cell flux is kept on thebasis of keeping the cell activity; and 3) a single cell (/cell cluster) capture and culture observation module solves the problem of cell tracking and positioning detection, and effectively culturesproliferative cells. The device is simple and convenient to operate, and provides equipment technical support for preparation and amplification of rare cell samples and single cell accurate researchin biomedicine and clinical diagnosis research.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly provides a high-throughput cell sorting and enriching device and its application method. Background technique [0002] Cells are the basic unit of living organisms. Tissues or body fluids of higher organisms (such as the human body) are composed of a variety of mixed cells. Separating and purifying target cells from mixed cell samples is an important step in current cell research. For biological research It is of great significance for both clinical and diagnostic analysis (such as tumor liquid biopsy). The detection and accurate evaluation of enriched tumor cells is clinically important, and can guide the staging and clinical management of cancer patients; currently, the clinical diagnosis of tumor cells is mainly through cytological analysis and invasive biopsy methods. The sensitivity of the method depends largely on the amount and quality of the fluid and the experience of the c...

AI Technical Summary

Problems solved by technology

It has been applied to the removal of large particle impurities and the separation of large and small cells in cell suspensions, and it also has a good effect on the enrichment of tumor cells. The disadvantages of low concentration and low enrichment purity due to easy clogging of pores during the filtration process limit its application in cell biology research and clinical diagnosis; in addition, various cost factors such as yield rate in practical applications are also important factors in the promotion of cell screening technology. an important obstacle

[0003] For the follow-up detection of recovered cells, traditional biological cell experiments are mostly completed in well plates, and then directly dropped on glass slides for observation, which is time-consuming and laborious. Reagents A large amount is used, and it is difficult to track and observe single cells and maintain a uniform microenvironment

Images