12 results about "Cell throughput" patented technology

The present invention provides an integrated microfluidic chip and a single cell culture, screening and export method thereof, the chip includes a base, a liquid inlet flow channel, a liquid outlet flow channel, a plurality of common flow channels and a plurality of functional units, wherein the two ends of the common flow channel are respectively connected to the liquid inlet flow channel and the liquid outlet flow channel, the functional unit includes a single cell introduction port, a cell culture screening chamber, a cell export chamber, a cell export port and a driving element, the driving element is used to provide power for the liquid to introduce the single cell entering the common flow channel into the cell culture screening chamber, and to export the target cell group cultured and screened in the cell culture screening chamber from the cell export port. The integrated microfluidic chip of the present invention can enable single cell culture, cell group identification and screening, and target cell group export to be completed on-chip, thereby improving cell throughput, simplifying experimental operations, reducing reagent usage and potential cross-contamination risks. At the same time, by combining thermal bubble printing technology, the entire process can be made more controllable, convenient and efficient.

The invention provides a construction method of a high-throughput sequencing library. The method comprises the following steps: carrying out nucleic acid interruption in a single-tube reaction chamber by adopting transposase with a universal joint, adding the universal joint, and then carrying out at least two rounds of ligation reactions in a branched-combination combination manner to introduce different tag combinations into genome fragments of each cell, thereby obtaining a sequencing library. The homogeneity of cell transposase breaking treatment is higher, the nucleic acid tag connection process is easier to operate, the cell flux covered by single sequencing is improved, the cost is lower, and the method is compatible with a single-cell multi-omics sequencing method.

The invention belongs to the technical field of biology, and particularly relates to high-throughput multi-mode index marking and sequencing detection on single-cell or subcellular nucleic acid substances. According to the invention, a microbead-coupled particle of a PCR primer containing a bar code is designed, the microbead-coupled particle can be delivered to a plurality of different reaction systems in a single reaction by means of droplet microfluidics and the like, and a specific bar code of the reaction system is added to a target nucleic acid molecule in the reaction system in a high-flux parallel manner; the single cell transcriptome or multi-omics sequencing database building is completed. The method disclosed by the invention has the advantages of large cell flux, simplicity and convenience in operation, low price, low pseudosingle cell rate, low no-load rate of a micro-reaction system, high data quality and strong applicability.

The embodiments of the present application relate to the field of mobile communication technology, and disclose a scheduling method, device, and storage medium. The method includes: obtaining the target physical resource block (PRB) requirement number and the scheduling number of the target user equipment; wherein the target PRB requirement number includes the sum of the PRB requirement numbers of the user equipment in the target cell where the target user equipment is located; the scheduling number includes the scheduling number of the target user equipment in the first cycle; when the target PRB requirement number exceeds the first PRB threshold and the scheduling number exceeds the preset scheduling threshold, the interference randomization of the target user equipment is disabled. For densely scheduled user equipment, the embodiments of the present application disable the interference randomization of the user equipment, reduce the probability of frequency domain resource truncation caused by downlink interference randomization, and improve spectrum utilization and cell throughput.

The present invention discloses an interference cancellation method, base station, device, and medium, including: determining that a victim cell is experiencing cross-slot interference; and interacting with an interfering cell to perform a first interference cancellation. This method can improve system performance, further reducing interference between macro and macro stations, macro and pico stations, and pico stations, thereby improving system performance and cell throughput.

The invention provides application of polyethylene glycol in reducing diffusion of cell contents in a single-cell suspension and improving single-cell capture efficiency. When the single-cell suspension containing polyethylene glycol is used for capturing single-cell nucleic acid, diffusion of contents of single cells, such as nucleic acid such as genome DNA or transcriptome RNA, can be effectively reduced, so that the contents of the single cells are more gathered, and the cell flux and capturing efficiency are effectively improved.

This invention provides a novel high-throughput single-cell multi-omics analysis method, comprising the following steps: fixation, lysis, nuclear permeation, and enzyme digestion of a population of cells; adding an A-tail and adjacent ligation to the DNA ends; and performing a transposition reaction. The pretreated cells are loaded onto a microfluidic platform, where single or multiple cells are co-encapsulated in droplets with beads containing capture adapters and a first molecular tag. The DNA and RNA of the same cell are simultaneously labeled using the capture adapters. The cells are released from the droplets and dispensed into multi-well plates. After decrosslinking, lysis, and DNA purification, primers with a second molecular tag are added to simultaneously amplify gDNA and cDNA, forming a unique composite tag. Library construction and sequencing are then performed. This invention improves cell throughput and data quality in a single experiment through a combined indexing approach, addressing the problems of low throughput, high cost, and significant ATAC signal background noise in existing single-cell three-omics technologies.

This invention relates to the field of cell processing, and more particularly to a cell processing module, method, and apparatus. The cell processing module includes a rotating platform, a first cell centrifuge container, and at least one second cell centrifuge container. The first cell centrifuge container is located at the center of the rotating platform and is coaxial with it. The second cell centrifuge containers are evenly distributed on the rotating platform, and the rotation centers of all the second cell centrifuge containers are on the rotation axis of the rotating platform. Thus, when the rotating platform rotates, it drives the first cell centrifuge container and at least one second cell centrifuge container to rotate with the platform, enabling multiple cell centrifuge containers to operate simultaneously. Since different cell centrifuge containers can be used to separate different target cells, the cell processing module provided by this invention can simultaneously separate different target cells, thereby increasing cell throughput and improving cell separation efficiency.

Provided herein in some aspects are methods, compositions, kits, and systems for performing multiplexed single-cell analysis on an in situ platform, providing alternatives which have a higher cell throughput and / or lower cost per cell compared to current single-cell analysis techniques. In some embodiments, the methods disclosed herein comprise using labeling agents that comprise sample-specific barcodes and / or cell feature specific barcodes (e.g., analyte specific barcodes) to label single-cell populations, immobilizing the labeled cells, and performing in situ detection of the labeling agents and / or other features including cellular analytes of the labeled cells.

To solve a problem in which a wireless communication system is evaluated on the basis of an average cell throughput of a system and / or its cell edge throughput, and a cell edge user experiences the low received signal strength, and the cell edge performance is affected by cell-to-cell interference, the frequency reuse limits the system interference when many or all cells perform transmission simultaneously on many or all time and frequency resources, and power boosting does not improve cell edge performance as both the serving cell signal strength and the interference signal strength are increased.SOLUTION: A method and a system for determining and communicating a CSI of one or more transmission points (channel state information (CSI) reference signal resources) are provided. The determination of the transmit state includes applying at least one transmit state parameter to the channel state information. Further reporting of the CSI based on the transmit state and / or at least one transmit state parameter applied thereto is contemplated.SELECTED DRAWING: Figure 4

The invention relates to a downlink antenna selection method and a base station, and belongs to the technical field of communication. The method comprises the following steps: acquiring FOE and RRU residence time corresponding to a user terminal in a cell; determining a user type corresponding to the user terminal according to the FOE and RRU residence time corresponding to the user terminal; determining a downlink transmission mode corresponding to the user terminal according to the user type corresponding to the user terminal; and in each RRU corresponding to the cell, determining a target RRU corresponding to the user terminal according to the downlink transmission mode corresponding to the user terminal and the SNR corresponding to each RRU. According to the invention, the receiving diversity gain in the same direction can be improved, the downlink interference is reduced, and the cell throughput is improved.

Embodiments of the present application provide a user equipment release method and device, network equipment and storage medium. The method comprises: in the case that it is detected that a user equipment fails to release resources occupied in a source cell, a master base station interacts with a core network and a secondary base station to release a context of the user equipment; and the master base station interacts with the user equipment to release a connection between the user equipment and the master base station. Therefore, in the embodiments of the present application, in the case that a user is hung, a release process of the user equipment is automatically triggered, thereby releasing the resources occupied by the user equipment in the source cell, and further improving NSA cell throughput and capacity, avoiding affecting the access of new users, and improving real user rate.