4 results about "Cell throughput" patented technology

The invention provides application of polyethylene glycol in reducing diffusion of cell contents in a single-cell suspension and improving single-cell capture efficiency. When the single-cell suspension containing polyethylene glycol is used for capturing single-cell nucleic acid, diffusion of contents of single cells, such as nucleic acid such as genome DNA or transcriptome RNA, can be effectively reduced, so that the contents of the single cells are more gathered, and the cell flux and capturing efficiency are effectively improved.

This invention provides a novel high-throughput single-cell multi-omics analysis method, comprising the following steps: fixation, lysis, nuclear permeation, and enzyme digestion of a population of cells; adding an A-tail and adjacent ligation to the DNA ends; and performing a transposition reaction. The pretreated cells are loaded onto a microfluidic platform, where single or multiple cells are co-encapsulated in droplets with beads containing capture adapters and a first molecular tag. The DNA and RNA of the same cell are simultaneously labeled using the capture adapters. The cells are released from the droplets and dispensed into multi-well plates. After decrosslinking, lysis, and DNA purification, primers with a second molecular tag are added to simultaneously amplify gDNA and cDNA, forming a unique composite tag. Library construction and sequencing are then performed. This invention improves cell throughput and data quality in a single experiment through a combined indexing approach, addressing the problems of low throughput, high cost, and significant ATAC signal background noise in existing single-cell three-omics technologies.

This invention relates to the field of cell processing, and more particularly to a cell processing module, method, and apparatus. The cell processing module includes a rotating platform, a first cell centrifuge container, and at least one second cell centrifuge container. The first cell centrifuge container is located at the center of the rotating platform and is coaxial with it. The second cell centrifuge containers are evenly distributed on the rotating platform, and the rotation centers of all the second cell centrifuge containers are on the rotation axis of the rotating platform. Thus, when the rotating platform rotates, it drives the first cell centrifuge container and at least one second cell centrifuge container to rotate with the platform, enabling multiple cell centrifuge containers to operate simultaneously. Since different cell centrifuge containers can be used to separate different target cells, the cell processing module provided by this invention can simultaneously separate different target cells, thereby increasing cell throughput and improving cell separation efficiency.

Embodiments of the present application provide a user equipment release method and device, network equipment and storage medium. The method comprises: in the case that it is detected that a user equipment fails to release resources occupied in a source cell, a master base station interacts with a core network and a secondary base station to release a context of the user equipment; and the master base station interacts with the user equipment to release a connection between the user equipment and the master base station. Therefore, in the embodiments of the present application, in the case that a user is hung, a release process of the user equipment is automatically triggered, thereby releasing the resources occupied by the user equipment in the source cell, and further improving NSA cell throughput and capacity, avoiding affecting the access of new users, and improving real user rate.