Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of donkey-hide gelatin in products for relieving cell oxidative stress damage

A technology of donkey-hide gelatin and cells, which is applied in the application field of donkey-hide gelatin in products used to relieve oxidative stress damage of cells, which can solve the problems of anti-oxidative stress and regulation of donkey-hide gelatin, and achieve the effect of alleviating common problems

Active Publication Date: 2022-05-24
BEIHANG UNIV +1
View PDF26 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no invention of donkey-hide gelatin anti-oxidative stress and regulation of related gene expression products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of donkey-hide gelatin in products for relieving cell oxidative stress damage
  • Application of donkey-hide gelatin in products for relieving cell oxidative stress damage
  • Application of donkey-hide gelatin in products for relieving cell oxidative stress damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) First, the commercial donkey-hide gelatin blocks are ground into small pieces by mechanical external force, dissolved in PBS, and conventional high-pressure sterilization (121 ° C, sterilization for 30 minutes) is performed in an autoclave. The main purpose of this process is to make The donkey-hide gelatin block is fully dissolved and the sterilized donkey-hide gelatin solution can be directly used for cell culture. The sterilized donkey-hide gelatin solution was prepared into a storage solution of 300 mg / ml with PBS, and centrifuged at a low speed (800 rpm / min) for 5 min to remove the grease and impurities in the upper layer. The prepared gelatin stock solution (concentration 300mg / ml) was stored at 4°C. Pre-warmed at 37°C when performing cell culture.

[0060] (2) LDH detected the toxic and side effects of different concentrations of donkey-hide gelatin on MEF cells cultured in vitro.

[0061] (a) Obtaining mouse embryonic fibroblasts:

[0062] Preparation of ...

Embodiment 2

[0081] Cluster analysis of Ejiao-regulated gene expression and correlation analysis of gene expression levels in primary MEF cells in each group:

[0082] 2.1. MEF cell processing and RNA extraction:

[0083] (a) Culture at 37°C, 5% CO 2 The MEF cells in the incubator were passaged, and the cells were cultured in 10% FBS (Gibco company fetal bovine serum product number: 10099-041), 5% PS (Gibco company penicillin-streptomycin double antibody product number: 15140122) high glucose DMEM culture (Cat. No.: 11995-065), the cells were prepared into a homogeneous cell suspension by pipetting with complete DMEM medium.

[0084] (b) MEF cells were counted using a hemocytometer at 60 × 10 4 The cells were seeded into 60mm cell culture dishes for culture, and at the same time, different concentrations of donkey-hide gelatin (0.3μg / ml, 3μg / ml, 30μg / ml, 300μg / ml) were added for pre-incubation, 24 hours after the cells adhered normally Different concentrations of donkey-hide gelatin (0....

Embodiment 3

[0094] Ejiao group and H 2 O 2 The number of differentially expressed genes in the injury group and Venn diagram analysis:

[0095] The experimental treatment method and RNA extraction method of mouse fibroblasts (MEF cells) were the same as in Example 2.

[0096] (1) The experimental results are as follows Figure 5 Show Venn diagram: each ellipse represents a comparative combination (such as the experimental group Ejiao versus H 2 O 2 Injury group), the numbers in the overlapping areas of the ellipses represent the number of common differential genes among the corresponding comparison combinations, and the non-overlapping areas represent the unique differential genes of each comparison combination. From the Venn diagram, the gene information common and unique to the comparison combination can be observed. As shown in the Venn diagram are three differential comparison groups.

[0097] 1: H 2 O 2 vs control;

[0098] 2: H 2 O 2 vs Ejiao;

[0099] 3: Ejiao vs cont...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of donkey-hide gelatin in a product for relieving cell oxidative stress damage, and relates to the technical field of biopharmaceuticals. The donkey-hide gelatin is used to prepare products that can regulate the expression of genes in cultured objects, and the genes that can be regulated include genes related to oxidative stress damage repair and stress response. Applying donkey-hide gelatin to the culture can alleviate the changes of oxidative stress damage on the gene expression level of the cultured subjects.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of donkey-hide gelatin in a product for relieving cell oxidative stress damage. Background technique [0002] Ejiao is a traditional Chinese medicine with a history of more than 2,000 years in China. It is made from black donkey skin. The main component is collagen degradation products, and it also contains polypeptides, polysaccharides, ketones, heterocyclic compounds and inorganic elements, etc. Ejiao has been used for more than 2,000 years to supplement blood and stop bleeding, promote hematopoiesis, dilate blood vessels, improve microcirculation, resist fatigue, delay aging, and improve human immunity. However, the pathogenesis of the above-mentioned diseases is related to oxidative stress injury. So far, there has been no invention of Ejiao anti-oxidative stress and related gene expression products. [0003] In view of this, the present inventio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61K35/36A61P31/00A61P9/10A61P25/28A61P25/14A61P25/16A61P25/00A61P29/00A61P21/00C12N5/077
CPCA61K35/36A61P31/00A61P9/10A61P25/28A61P25/14A61P25/16A61P25/00A61P29/00A61P21/00C12N5/0656C12N2500/84
Inventor 韩晶刘会生周祥山田守生
Owner BEIHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com