Method for constructing mouse preeclampsia model

Abstract

The invention belongs to the field of biomedicines, and relates to a method for constructing a preeclampsia (PE) model and a new purpose of Gal-9 as a biologic marker for preparing a forecast and prognosis assessment platform of PE. Based on characteristic pathological changes as well as etiology and pathogenesis of attack of PE, and based on assessing of clinical manifestation, organ function damaged state and development of embryo placentas, a mouse model most similar to PE clinical indications is constructed and obtained. A quantifying observation index of the model, constructed by the invention, is suitable for researching early nosogenesis of PE, assessing PE onset risk, forecasting prognosis of mothers and children, and assessing curative effect end points before clinical applicationof medicines, and a standard platform is provided for further researching and comparing advantages and disadvantages of different medicines.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a method for constructing an animal model, in particular to a method for constructing a mouse preeclampsia (PE) model, and Galectin-9 (Gal-9) is used as a biomarker for preparing preeclampsia (PE) new uses in the prediction and prognosis evaluation platform. The model established by the present invention is suitable for quantitative index observation and research on preeclampsia (PE), including: early pathogenesis, PE risk assessment, maternal and child prognosis prediction, and drug preclinical efficacy endpoint assessment, etc., and for further research to compare different The advantages and disadvantages of drugs provide a standardized platform. Background technique [0002] The prior art discloses that preeclampsia (PE) is a pregnancy-specific syndrome involving multiple systems and the fetus. In clinical practice, it is mainly characterized by high blood pressure, proteinuria, and ...

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Problems solved by technology

For example, on the 8th or 9th day of pregnancy, rats were injected with adenovirus carrying sFlt-1 into the tail vein, and PE-like changes such as blood pressure elevation, proteinuria, and kidney lesions appeared at the end of pregnancy; multiple hypoxia-inducible factor 1α (HIF-1α ) overexpression model has also been applied to the pathogenesis of preeclampsia (PE) oxidative stress; inflammatory response and cytokine stimulation such as LPS or inflammatory factors TNF-α, IL-6 injection of rats can successfully establish preeclampsia (PE) ) models, etc.; however, due to the diversity of PE pathophysiology and clinical manifestations, there is no single animal model that can effectively simulate all the characteristics of preeclampsia (PE), especially the lack of features that can reflect the characteristics of preeclampsia (PE). Preeclampsia (PE) research model of pathological changes and disease progression. In addition, some inducers will also cause hypertension and proteinuria that are not pregnancy-specific when applied during non-pregnancy. Therefore, the application of the model is limited

[0006] Galectin-9 (Gal-9) is a member of the lectin family and is widely expressed in a variety of tissues; so far there has been no research report on the relationship between Gal-9 and the pathological changes and pathogenesis of preeclampsia (PE)

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