A rapid visual detection method based on water-soluble peryleneimide derivatives
A technology of perylene imide and derivatives is applied in the field of rapid visual detection based on water-soluble perylene imide derivatives, which can solve the problems of low detection efficiency and low sensitivity, and achieve good water solubility, high detection efficiency and sensitivity. Effect
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[0045] Preparation of standby material:
[0046] Preparation of buffer solution: Weigh the tris solid, prepare 500 mL of Tris buffer solution with a concentration of 1 mmol / L with distilled water, and adjust its pH value to 9.0 with 1 mol / L sodium hydroxide standard solution. Store it in a 4°C refrigerator for later use.
[0047] Preparation of probe mother solution: Weigh the solid described in formula (I), prepare a mother solution with a concentration of 1 mmol / L with distilled water, divide it into solutions of the same volume and put them in vials for later use. Spectrum test with prepared Tris buffer solution (1mmol / L, pH = 9.0) diluted to a certain concentration for testing.
[0048] Preparation of analytes and interfering substances: Folic acid was prepared with distilled water into a stock solution with a concentration of 1 mmol / L for later use. Other interfering substances were prepared into 1mmol / L stock solution with distilled water. These solutions were stored ...
Embodiment 1
[0051] Preparation of compounds represented by formula (I). Take 784mg of perylene anhydride and react with 6mL of tris(2-aminoethyl)amine, stir the reaction at 100°C for 24h, then react at 120°C for 4h. After the reaction was completed, it was cooled to room temperature, and ethanol / ether (1:3) was added to precipitate the product, followed by suction filtration. After suction filtration, it was washed three times with toluene and ethyl acetate respectively, and dried under vacuum at 40° C. to obtain the compound described in (IV). Take 276 mg of the substance described in (IV), add 1.28 mL of 85% formic acid and 0.88 mL of 30% formaldehyde to it, stir at room temperature for 1 h, and then heat at 120° C. for 16 h. After cooling to room temperature, 30 mL of anhydrous diethyl ether was added for washing, and the washing was repeated 3 times to obtain the compound described in (V). Take 150mg of the compound described in (V), add 6mL of methanol and 100m of sodium carbonate ...
Embodiment 2
[0055] Fluorescence spectrum test :
[0056] Take 3 μL of the probe mother solution and 950 μL of 1 mmol / L Tris buffer solution and mix them into a 1 mL sample cell. After mixing evenly, measure the fluorescence spectrum of the probe buffer solution, and record the fluorescence intensity as I at a wavelength of 548 nm. 0 . Subsequently, gradually add folic acid with a certain concentration gradient into the sample pool, measure the corresponding fluorescence spectrum after mixing evenly, and record the fluorescence intensity at 548nm as 1.
[0057] The result is as figure 1 shown (i.e., figure 1 Indicates, in Tris (1mmol / L, pH=9.0) buffer solution, the degree of quenching of the fluorescence of the aforementioned peryleneimide derivatives at 548nm with the concentration of folic acid. QI=[(I 0 -I) / I 0 ]×100%,λ ex =498nm, the slit width of excitation light and emission light is 5.0nm. )
[0058] The aforementioned peryleneimide derivatives are excited at an excitation...
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