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A kind of probiotic microcapsule and its preparation method and application

A technology of probiotics and microcapsules, which is applied in the field of microbial preparations, can solve the problems of inability to effectively embed probiotics, difficulty in reaching the colon, and inactivation of probiotics, so as to achieve high shelf-life survival rate, prolong residence time, and improve survival rate effect

Active Publication Date: 2022-04-15
北京诺葆营养科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are still many technical defects in the existing probiotic embedding technology, such as the use of chemical cross-linking agents and organic solvents in the synthesis process of the carrier, which will cause hidden dangers to product safety; since the individual diameter of probiotics is usually 1-10 μm However, some carriers such as micro-nano emulsions and biological micro-nanoparticles cannot effectively embed probiotics due to their small size, and some carrier substances such as tablets or capsules cannot be directly worn due to their large size. Passing through the pyloric sphincter, causing the carrier to be digested and degraded in the stomach and release the probiotics, which will cause the inactivation of the probiotics and make it difficult to reach the colon. At the same time, such oversized carrier substances will also affect the food and sensory of the product quality; in addition, the existing carrier system usually has a porous structure on the surface, which will lead to the extremely small size of H in the digestive environment of the stomach + (<1nm) and digestive enzymes (<5nm) smoothly pass through the pores on the surface of the carrier and diffuse into its interior, resulting in the degradation and inactivation of the embedded probiotics, losing the effective protective effect of the carrier on the probiotics

Method used

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  • A kind of probiotic microcapsule and its preparation method and application
  • A kind of probiotic microcapsule and its preparation method and application
  • A kind of probiotic microcapsule and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This embodiment provides a probiotic microcapsule and a preparation method thereof, including the following three embedding steps:

[0053] S1. Disperse 1.5g of carrageenan powder in deionized water, stir at 60°C for 3h to achieve complete dissolution, then add 0.2g of glucose and 0.1g of glycerin to the solution in turn, mix and stir for 45min, then mix the solution at 121 ℃ for 20min, then cooled to 25℃, and 1g contains 10 live bacteria 10 cfu / g of probiotic bacteria powder (Lactobacillus plantarum Lp3a (CGMCC 17054), purchased from Jiangsu Baiaoda Biotechnology Co., Ltd.) was dissolved in the above-mentioned mixed solution after sterilization;

[0054] S2. The first layer of embedding: Dissolve 15g of whey protein in deionized water, stir at 45°C for 3 hours to ensure that the whey protein is fully hydrated, and then sterilize it at 121°C for 20 minutes and then cool it to 25°C; Then slowly add the above-mentioned mixed solution containing probiotics into the whey p...

Embodiment 2

[0073] This example provides a probiotic microcapsule and its preparation method, and its performance is tested. The specific preparation process and test method are the same as in Example 1, the only difference is that in the premix, the concentration of carrageenan is 3%, the concentration of glucose is 0.25%, the concentration of glycerin is 0.1%, and the concentration of whey protein is 10%. %, the concentration of probiotic bacteria powder is 1%. In the second layer of embedding solution, the concentration of pectin was 3%, and the concentration of xanthan gum was 0.1%. In the third layer of embedding solution, the enteric coating material is hydroxypropylmethylcellulose acetate succinate with a concentration of 1%. Adjust the pH of the mixture to 3 in S2.

[0074] The obtained probiotic microcapsules have a particle diameter of 560-800 μm; at a room temperature of 20-30° C., the embedding survival rate is 98.7 percent; and the one-year shelf-life survival rate is 93.5 ...

Embodiment 3

[0081] This example provides a probiotic microcapsule and its preparation method, and its performance is tested. The specific preparation process and test method are the same as in Example 1, the only difference is that in the premixed solution, the concentration of carrageenan is 1%, the concentration of glucose is 0.18%, the concentration of glycerin is 0.07%, and the concentration of whey protein is 30%, and the concentration of probiotic bacteria powder is 1%. In the second layer of embedding solution, the concentration of pectin was 1%, and the concentration of xanthan gum was 0.066%. In the third layer of embedding solution, the enteric coating material is hydroxypropylmethylcellulose acetate succinate with a concentration of 7%. In S2, adjust the pH of the mixture to 5.5.

[0082] The obtained probiotic microcapsules have a particle diameter of 450-670 μm; at a room temperature of 20-30° C., the embedding survival rate is 97.8 percent; and the one-year shelf-life surv...

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Abstract

The invention relates to the technical field of microbial preparations, and specifically discloses a probiotic microcapsule, a preparation method and application thereof. The probiotic microcapsules of the present invention include a sclerotia protective layer and an enteric coating layer for embedding probiotics in the center, and also include a coating isolation layer at least partially wrapping the sclerotia protective layer; the coating The raw materials of the isolation layer include pectin and xanthan gum, and the raw materials used for embedding the probiotics in the sclerotia protective layer include whey protein and carrageenan. The probiotic microcapsules of the present invention can significantly increase the live bacteria content of the probiotics in the shelf life, and at the same time, release most of the probiotics in the intestinal tract, thereby exerting beneficial effects.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a probiotic microcapsule and its preparation method and application. Background technique [0002] Probiotics are a general term for a class of active microorganisms that colonize the host's intestinal tract or reproductive system and can produce definite beneficial effects by improving the host's intestinal microecological balance. They can improve food digestibility, produce nutrients, and maintain intestinal flora Balance, inhibit the growth of pathogenic bacteria, and then play the role of enhancing nutrition, regulating immunity, and preventing diseases. [0003] Probiotics are usually extremely sensitive to environmental conditions, such as oxygen, gastric acid, enzymes, bile salts, etc. These unfavorable conditions will seriously limit their beneficial effects on human body. Therefore, effective embedding technology strategies for probiotics can be used to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/64A61K9/62A61K9/56A61K47/42A61K47/36A61K47/26A61K47/10A61K47/38A61K35/741A61K35/747A61P1/00A61P1/14A61P37/02A61P3/02A23L33/135A23P10/30A23P20/18
CPCA61K9/5052A61K9/5036A61K9/5015A61K9/5047A61K9/5073A61K9/5089A61K35/741A61K35/747A61P1/00A61P1/14A61P37/02A61P3/02A23L33/135A23P10/30A23P20/18A23V2002/00A23V2200/32A23V2200/30A23V2250/5072A23V2250/5086A23V2250/5036A23V2250/54252
Inventor 张焕丽张旗张泽炎陶秀梅陈鹏
Owner 北京诺葆营养科技有限公司
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