Rapid respiratory tract microorganism detection system and method

A detection system and detection method technology, applied in biochemical equipment and methods, biological material sampling methods, enzymology/microbiology devices, etc., can solve the impact of the aviation industry, the inability to quickly detect latent infections, resistance, etc. problem, to achieve the effect of high sensitivity, strong operability and short time

Inactive Publication Date: 2020-08-14
HUNAN UNIV
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Problems solved by technology

[0004] At present, the routine detection measures for SARS-CoV-2 virus infection mainly include body temperature monitoring, viral nucleic acid-fluorescence quantitative PCR detection, serum antigen antibody colloidal gold method or ELISA method detection, etc., these measures cannot realize the rapid detection of SARS-CoV Latent infection of -2 virus
[0005] In addition, it is worth noting that due to the development of urbanization and globalization, human beings are gathering more and more closely together, and the

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  • Rapid respiratory tract microorganism detection system and method

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Embodiment 1

[0037] Please combine figure 1 , This embodiment provides a rapid detection system for respiratory microorganisms, including a gas sampling bag, a microorganism sampler, a microorganism enrichment and concentration device, an immune chip, and a photodetector.

[0038] In this embodiment, the gas sampling bag is used to collect exhaled gas as a sample to be tested. The microbial sampler is connected with the gas sampling bag, which is used to enrich the microorganisms in the sample to be tested into the capture liquid. The microorganism enrichment device is used to concentrate the microorganisms enriched in the capture liquid. The immune chip is used for antigen-antibody reaction with microorganisms. The photosensitive detector is used to detect the reaction products of antigen and antibody.

[0039] Preferably, the microbial sampler is a biosampler microbial sampler, matched with a special capture liquid.

[0040] Preferably, the microorganism enrichment and concentration ...

Embodiment 2

[0045]The present embodiment provides a rapid detection method for respiratory microbes. Taking the detection of SARS-CoV-2 virus as an example, the rapid detection system for respiratory microbes in Example 1 comprises the following steps:

[0046] S1, collect 0.5 L of exhaled gas by means of an air sampler or directly using a gas collection bag (Shanghai Huibin Instrument Co., Ltd., China), preferably 0.3-1.0 L of exhaled gas, as a sample to be tested.

[0047] S2, using a biosampler microbial sampler (biosampler, SKC company, the United States) to enrich the microorganisms in the gas sample to be tested into 20 mL of a special capture solution to obtain a microorganism enrichment solution.

[0048] S3, use diluents such as physiological saline to dilute the microbial enrichment solution 10 times, and then use the CP Select microbial rapid enrichment system (Innovaprep, USA) to concentrate to about 150 μL, preferably to 100-200 μL, to obtain a microbial concentrate.

[0049]...

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Abstract

The invention relates to a rapid respiratory tract microorganism detection system and method. The rapid respiratory tract microorganism detection system comprises a gas sampling bag used for collecting exhaled gas, a microorganism sampler, a microorganism enrichment and concentration device, an immunochip and a light sensation detector. According to the rapid respiratory tract microorganism detection system, a gas sample to be detected is collected through a respiration method, and pathogenic microorganisms propagated through the respiratory tract can be rapidly, specifically and highly sensitively detected in cooperation with immunochip and light sensation detection through enrichment and concentration, and early detection of high-pathogenicity and infectivity pathogenic microorganisms (such as SARS-CoV-2 and the like) propagated by the respiratory tract can be performed on large-scale activity participants and passengers of vehicles for land transportation, sea transportation, air transportation and the like, so that accurate judgment and powerful support can be provided for epidemic prevention and control.

Description

technical field [0001] The invention relates to the field of detection of pathogenic microorganisms, in particular to a rapid detection system and method for respiratory microorganisms. Background technique [0002] Highly pathogenic pathogenic microorganisms transmitted through the respiratory route, such as SARS-CoV-2 virus, often easily lead to rapid and large-scale spread of the epidemic, which has a very serious impact on human production, life and life and health. [0003] For infectious diseases, confirmed patients or "symptomatic infected persons" (group A) and their close contacts (group C) can effectively prevent and control the spread of the infectious disease through strict isolation measures, but However, there are great difficulties in the prevention and control of "asymptomatic infected persons" (category B population). The latest research in the journal Science on March 16, 2020 shows that the main cause of the large-scale spread of the SARS-CoV-2 virus is t...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/00G01N33/569G01N33/543
CPCC12M23/14C12M33/00G01N33/54306G01N33/54313G01N33/56983G01N2333/165
Inventor 唐松青郑悦王安琪樊苏萍李丹刘智超
Owner HUNAN UNIV
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