Method for rapidly detecting retrovirus by using cell ultrathin section electron microscope
A retrovirus and ultra-thin section technology, which is applied in the field of rapid detection and judgment of unknown virus morphology, rapid cell ultra-thin section electron microscope detection of retroviruses, and can solve the problem that the ultra-thin section electron microscope technology cannot be quickly detected and prepared. Complicated, unapplied and other problems, to achieve the effect of fast detection method, reduce interference factors, reduce time cost and labor cost
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[0036] 1. Experimental steps:
[0037] The present invention provides a kind of fast cell ultrathin section electron microscope detection retrovirus technology, and it comprises the following steps:
[0038] Step 1: Gather 1-2×10 7 Each cell sample was washed 3 times with 1×PBS, fixed with 5% glutaraldehyde for 12 hours, and then fixed with 1% osmic acid for 30 minutes;
[0039] Step 2: Stain with uranyl acetate block and place in a refrigerator at 2-8°C for 2-3 hours to reduce the shaking of the sample;
[0040] Step 3: Acetone dehydration and overtreatment, 70% acetone, 90% acetone, and 100% acetone are dehydrated for 5 minutes each, and should be placed on a shaker during dehydration;
[0041] Step 4: Acetone: Epoxy resin = 1:1 Infiltrate for 20 minutes and then pure resin infiltrate for 2 hours. This step needs to be placed on a shaker;
[0042] Step 5: Polymerize the sample in pure resin at 60°C for at least 10 hours;
[0043] Step 6: Ultra-thin slices of 70-100nm, ca...
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