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Quick detection and analysis method for anti-cancer drugs in sludge of sewage treatment plant

A technology for sewage treatment plants and anti-cancer drugs, which is applied to the detection of anti-cancer drugs in solid media and the detection of anti-cancer drugs, can solve the problems of high cost and complex process, and achieve high repeatability, accurate results and repeatability. Good results

Active Publication Date: 2020-08-18
SHANGHAI UNIV
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  • Description
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Problems solved by technology

[0004] In order to solve the problems of the prior art, the purpose of the present invention is to overcome the deficiencies in the prior art, to provide a rapid detection and analysis method for anticancer drugs in the sludge of sewage treatment plants, which can carry out the pretreatment of anticancer drugs in the sludge , to improve the technical problems of complex process and high cost of existing processing methods

Method used

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  • Quick detection and analysis method for anti-cancer drugs in sludge of sewage treatment plant
  • Quick detection and analysis method for anti-cancer drugs in sludge of sewage treatment plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this example, the standard curve of the substance cyclophosphamide was drawn. figure 1 It is the standard curve diagram of the substance cyclophosphamide, the standard curve equation is y=171326x+55701, and the correlation coefficient is R 2 is 0.9996.

Embodiment 2

[0030] In the present embodiment, the standard addition recovery experiment is carried out, and the recovery rate of anticancer drugs in the sludge sample is tested by the internal standard method, and the steps are as follows:

[0031] a. Sample pretreatment: freeze-dry the sludge sample, grind it into powder, pass through a 100-mesh filter sieve, weigh a certain amount of sieved soil sample and put it into a centrifuge tube, add internal standard cyclophosphamide-D4, Add 20 ml of methanol, centrifuge, repeat three times, pour the supernatant into the prepared nitrogen blowing test tube each time; blow the liquid in the nitrogen blowing tube to 1 mL, then pour it into a beaker with 100 mL of ultrapure water, and at the same time Repeatedly clean the wall of the nitrogen blowpipe three times, and pour the cleaned water into the beaker; set up three parallel samples, take 0.2g of each sample, and add cyclophosphamide-D4 with a concentration of 100μg / L in the first and second gro...

Embodiment 3

[0041] This embodiment is basically the same as Embodiment 2, and the special features are:

[0042] In this embodiment, a rapid detection and analysis method for anticancer drugs in the sludge of a sewage treatment plant, the determination of the concentration of cyclophosphamide in the actual sample is carried out. 5 parts of water samples in the environment are randomly collected, and the method in step 1 of the present invention is used to carry out Pretreatment, and then use the solid phase extraction method in step 2 of the present invention to purify and enrich, and then use the method of high performance liquid chromatography-mass spectrometry in step 3 of the present invention to analyze and detect the actual concentration of the target substance in the sample, including the following steps :

[0043] a. Sample pretreatment:

[0044]Freeze-dry the sludge sample, grind it into powder, pass through a 100-mesh filter sieve, weigh a certain amount of sieved soil sample a...

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Abstract

The invention discloses a quick detection and analysis method for anti-cancer drugs in sludge of a sewage treatment plant, which can efficiently and quickly detect five anti-cancer drugs in the sludgeat the same time by combining a refrigerated centrifugation-solid phase extraction column with a high performance liquid chromatography-mass spectrometry method, including cyclophosphamide, isocyclophosphamide, methotrexate, irinotecan and capecitabine. The method comprises the following steps: freeze-drying sludge, weighing, adding an internal standard substance, dissolving, centrifuging, blowing nitrogen and redissolving; then extracting and purifying five anti-cancer drugs in the sample by using a solid-phase extraction small column; and detecting the content of the target object in the sample through a high performance liquid chromatography-mass spectrometer. The method is simple in solid sample treatment step, convenient to operate, good in stability, capable of quickly obtaining thesolid sample and suitable for the sample detected by the liquid chromatograph-mass spectrometer; the method is low in sample pretreatment cost, high in detection speed, high in automation degree, sensitive in response, high in recovery rate and convenient for industrial application.

Description

technical field [0001] The invention relates to a detection method of anticancer drugs, in particular to a detection method of anticancer drugs in solid media, which is applied to the technical field of trace detection of organic pollutants in solid media. Background technique [0002] Current cancer treatment methods mainly include surgery, chemotherapy, radiotherapy and immunotherapy, etc. Whether used alone or in combination with surgery or radiotherapy, chemotherapy is one of the most widely used cancer treatment options, and chemotherapy involves various anticancer drug use. With the arrival of population aging and the deterioration of the ecological environment, the prevalence of cancer is also increasing, so the use of anticancer drugs is also increasing, including cyclophosphamide, ifosfamide, methotrexate , irinotecan and capecitabine are the most used drugs. Anticancer drugs that have not been metabolized and degraded by the human body enter the sewage treatment ...

Claims

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Application Information

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IPC IPC(8): G01N30/08G01N30/88
CPCG01N30/08G01N30/88
Inventor 杨晓李栋邹齐张芝勃
Owner SHANGHAI UNIV
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