Preparation method of bioactive walnut shell polyphenol nano transfer system

A biologically active, walnut shell technology, which is applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, food science, etc., can solve the problem of poor solubility, low bioavailability, and unstable properties of walnut shell polyphenols and other problems, to achieve the effect of shortening the purification cycle, good sustained release performance, and simple and convenient production process

Active Publication Date: 2020-08-25
南京恒舟科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor solubility, unstable properties, and low permeability of walnut shell polyphenols in aqueous media, resulting in low bioavailability, their applications in functional food and pharmaceutical fields are still limited.

Method used

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  • Preparation method of bioactive walnut shell polyphenol nano transfer system
  • Preparation method of bioactive walnut shell polyphenol nano transfer system
  • Preparation method of bioactive walnut shell polyphenol nano transfer system

Examples

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preparation example Construction

[0038] The invention discloses a preparation method of a biologically active walnut shell polyphenol nanometer transfer system, which comprises the following steps:

[0039] 1. Add walnut shell powder to the ethanol aqueous solution with ethanol volume fraction of 80% according to the ratio of solid to liquid of 1:20, and carry out ultrasonic-assisted extraction. After centrifugation, the centrifugation condition is 8000 rpm for 15 minutes; the walnut shell polyphenol extract is purified by macroporous resin column chromatography. , The elution flow rate is 3.0mL / min, and the elution volume is 2BV; the eluate is concentrated and then freeze-dried to obtain purified walnut shell polyphenols;

[0040] 2. Fully dissolve the walnut shell polyphenols purified in step 1 in an ethanol aqueous solution with an ethanol volume fraction of 80%, and centrifuge to remove insoluble matter. The centrifugation condition is 3000 rpm for 10 minutes, and the walnut shell polyphenols with a conce...

Embodiment 1

[0047] A preparation method of bioactive walnut shell polyphenol nano delivery system, comprising the following steps:

[0048] 1. Add walnut shell powder to the ethanol aqueous solution with ethanol volume fraction of 80% according to the ratio of solid to liquid of 1:20, and carry out ultrasonic-assisted extraction. After centrifugation, the centrifugation condition is 8000 rpm for 15 minutes; the walnut shell polyphenol extract is purified by macroporous resin column chromatography. , The elution flow rate is 3.0mL / min, and the elution volume is 2BV; the eluate is concentrated and then freeze-dried to obtain purified walnut shell polyphenols;

[0049] 2. Fully dissolve the walnut shell polyphenols purified in step 1 in an ethanol aqueous solution with an ethanol volume fraction of 80%, centrifuge to remove insoluble matter, and centrifuge at 3000rpm for 10 minutes to prepare a walnut shell polyphenol solution with a concentration of 50 μg / mL ;

[0050] 3. Add the polysacc...

Embodiment 2

[0055] The process steps of embodiment 2 are the same as that of embodiment 1, the difference is that in step 4, the addition amount of the aqueous solution of polysaccharide polysaccharide in embodiment 2 is 20mL, and the rate of addition is 1 drop / s.

[0056] The walnut shell polyphenol-element mushroom polysaccharide nanopowder particle that embodiment 2 prepares is uniform, and average particle size is 234.4nm, from image 3 It can be seen that walnut shell polyphenols-Mu polysaccharide nanopowder has high stability under ultraviolet irradiation, and the polyphenol retention rate is 91.0%; walnut shell polyphenols from Figure 4 It can be seen that the nanoparticles have a good sustained-release effect in simulating the digestion process of the gastrointestinal tract. 28.72% are released in the gastric digestion stage, and 72.82% are finally released after digestion in the small intestine. The transmission electron microscope results are as follows: Figure 10 , the scanni...

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Abstract

The invention relates to a preparation method of a bioactive walnut shell polyphenol nano transfer system. The preparation method comprises the following steps of 1, adding walnut shell powder into anethanol water solution, carrying out ultrasonic-assisted extraction, concentration and centrifugation, purifying the walnut shell polyphenol extract, and carrying out freeze drying to obtain the purified walnut shell polyphenol; 2, fully dissolving the walnut shell polyphenol purified in the step 1 in the ethanol water solution, and centrifuging to remove insoluble substances; 3, adding panellusserotius polysaccharide into deionized water, and fully dissolving through magnetic stirring; 4, slowly dropwise adding the panellus serotius polysaccharide aqueous solution into the walnut shell polyphenol solution, and performing stirring to obtain a walnut shell polyphenol-panellus serotius polysaccharide nano-mixed system; and 5, performing centrifuging and drying on the walnut shell polyphenol-panellus serotius polysaccharide nanometer mixed system to obtain the walnut shell polyphenol-panellus serotius polysaccharide nanometer powder. The nano transfer system prepared by the invention has high stability and good slow release performance, and can be widely applied to the fields of functional food and medicines.

Description

technical field [0001] The invention relates to a preparation method of a biologically active walnut shell polyphenol nanometer delivery system, which belongs to the technical field of functional food and medicine. Background technique [0002] Polyphenols are a class of polyhydroxy compounds widely distributed in a variety of plants. Polyphenols are generally divided into water-soluble and water-insoluble phenolic compounds. Studies have found that walnut shell polyphenols have a variety of health functions, such as anti-cancer, anti-inflammatory, antibacterial and reduce the risk of cardiovascular diseases. Studies have found that these biological activities are closely related to the strong antioxidant activity of walnut shell polyphenols, which can exert their antioxidant effects by activating and strengthening the endogenous defense system. However, due to the poor solubility, unstable properties and low permeability of walnut shell polyphenols in aqueous media, resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61K9/52A61K47/36A61P35/00A61P29/00A61P31/04A61P31/10A61P9/00A23L33/105A23L33/125A23L29/30A23P10/30
CPCA23V2002/00A61K9/5161A61K36/52A61K2236/333A61K2236/53A61K2236/55A23L29/30A23L33/105A23L33/125A23P10/30A61P9/00A61P29/00A61P31/04A61P31/10A61P35/00A23V2200/30A23V2250/2132A23V2250/51A23V2300/14A23V2300/10A23V2300/20A23V2300/48
Inventor 李小雨周鹏王璐冯茹罗珍
Owner 南京恒舟科技有限公司
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