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Method for oxidizing TMB by taking nano cerium dioxide as simulated oxidase

A nano-ceria, oxidase technology, applied in nanotechnology, chemical analysis by catalysis, material analysis by observing the impact on chemical indicators, etc., to achieve simple synthesis steps, easy reaction temperature, and high purity. Effect

Inactive Publication Date: 2020-08-25
SHANDONG UNIV OF TECH
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, there is no report on the use of nano-ceria as a simulated oxidase for catalytic oxidation of TMB in the prior art

Method used

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  • Method for oxidizing TMB by taking nano cerium dioxide as simulated oxidase
  • Method for oxidizing TMB by taking nano cerium dioxide as simulated oxidase
  • Method for oxidizing TMB by taking nano cerium dioxide as simulated oxidase

Examples

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Embodiment 1

[0022] Dissolve 0.22 g of cerium sulfate tetrahydrate and 0.3 g of sodium hydroxide in 50 ml of deionized water, respectively. Dissolve polyvinylpyrrolidone in cerium sulfate tetrahydrate solution, and stir continuously at room temperature for 30 minutes until polyvinylpyrrolidone is completely dissolved. Add sodium hydroxide solution dropwise to the above solution, continue to stir at room temperature for 30 minutes, centrifuge, wash with deionized water and absolute ethanol three times, and dry at 30°C to obtain a light yellow powder.

[0023] The nano-cerium oxide obtained in the above examples was used to catalyze the TMB color reaction, and the specific implementation was as follows: 16 mg of TMB was dispersed in 7.5 ml of a series of pH gradients (pH gradients were 5.4, 6.0, 6.4, 7.0, 7.4) In the phosphate buffer solution, the solution system I was formed; 1.6 mg of the nano-cerium oxide simulated oxidase prepared in the example was added to the solution II, slightly sha...

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Abstract

The invention discloses a method for oxidizing TMB (3, 3', 5, 5'-tetramethyl benzidine) by taking nano cerium dioxide as simulated oxidase, which comprises the following steps: taking cerium sulfate tetrahydrate, polyvinylpyrrolidone and sodium hydroxide as raw materials, and preparing at room temperature; the nano cerium dioxide is used as a catalyst, oxygen is directly catalyzed to oxidize TMB into a blue-green substance within a certain pH range, and the method is a TMB catalytic oxidation method capable of replacing natural enzymes. The method belongs to the technical field of mimic enzymecatalytic oxidation, and the nano cerium dioxide mimic oxidase has the advantages of simple synthesis steps, low synthesis cost, quick pH response, remarkable oxidation effect and the like, and has important application prospects in the fields of biological catalytic oxidation, medical immunodetection and the like.

Description

technical field [0001] The invention provides a method for catalyzing and oxidizing TMB by using nanometer ceria as a simulated oxidase, and belongs to the technical field of simulated enzyme catalyzed oxidation. Background technique [0002] Natural enzymes play a role in maintaining metabolism, genetic information transmission and other biochemical processes in organisms, and are also catalysts necessary to catalyze various chemical reactions in the body. Most of the natural enzymes in organisms are composed of proteins, which require specific temperature and pH to catalyze chemical reactions in the body. Natural enzymes have high efficiency and specificity, but they have disadvantages such as high production cost in vitro, and their properties are easily affected by environmental factors. The artificially synthesized nano-mimetic enzymes with natural enzyme characteristics have the advantages of stable structure, stable properties, simple synthesis process, and reusabili...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78G01N23/207G01N31/10B82Y40/00
CPCB82Y40/00G01N21/33G01N21/78G01N23/207G01N31/10
Inventor 李晓伟周浩王东耿欣温广武张丽娟
Owner SHANDONG UNIV OF TECH
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