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Bionic tissue engineering scaffold and preparation method thereof

A technology for tissue engineering scaffolds and corneas, which can be used in tissue regeneration, non-woven fabrics, and pharmaceutical formulations. It can solve problems such as inability to effectively guide repair and regeneration, large differences in ECM structures, and poor mechanical strength of scaffolds. It achieves excellent results. Biocompatibility and degradability, enhanced in vivo safety, favorable adhesion effect

Active Publication Date: 2020-09-01
YIGONG RUISI FUJIAN ENG RES CENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The disadvantages of electrospinning technology in terms of materials are: most of the electrospinning materials are artificially synthesized polymer materials, and the materials mainly include polyglycolide, polylactide and their copolymers, etc.
However, the ECM in these two patents only plays a role of modification in the electrospinning scaffold, and the main component of the scaffold is still a synthetic polymer material, which still cannot avoid the cytotoxicity generated during the degradation process.
This is because the mechanical strength of the ECM scaffold is not good, the stability is not good and it is easy to degrade, so it is difficult to exist stably in the body
[0005] 2. The structure of ECM imitated by electrospinning technology is single, which is quite different from the complex ECM structure in the body. For some complex human tissues, the imitated ECM cannot effectively play a role in guiding repair and regeneration in vivo

Method used

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  • Bionic tissue engineering scaffold and preparation method thereof
  • Bionic tissue engineering scaffold and preparation method thereof
  • Bionic tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0051] The invention provides a biomimetic tissue engineering scaffold, which comprises more than one kind of ECM extraction material with a weight of more than 90%, and the ECM extraction material is extracted from biological tissue.

[0052] The bionic engineering scaffold in this embodiment includes more than one kind of ECM extraction material extracted from biological tissues with a weight of more than 90%, which reduces the by-products generated during the degradation process of artificially synthesized polymer materials, and effectively improves the efficiency of the bionic engineering scaffold. Human biocompatibility.

[0053] ECM extraction materials are extracted from biological tissues, which can be but not limited to bone, skeletal muscle, cornea, liver and skin.

[0054]In a bionic engineering scaffold, a combination of more than two ECM extraction materials can be used, specifically, it can be a combination of ECM extraction materials extracted from decalcified b...

Embodiment approach 2

[0059] The invention provides a method for preparing a bionic tissue engineering scaffold, which comprises the following steps: adding a cross-linking agent to more than one ECM extraction material before electrospinning to generate a cross-linked material through an in-situ cross-linking reaction, and making the cross-linked material Made bionic tissue engineering scaffold.

[0060] Wherein, the cross-linking agent is but not limited to one or a combination of two or more of formaldehyde, glutaraldehyde and transglutaminase.

[0061] Wherein, the addition amount of each component in the cross-linking agent is the following percentage by weight of the ECM extraction material: 0.2%-4% of formaldehyde, 1%-3% of glutaraldehyde or 2%-6% of transglutaminase.

[0062] In order to improve the performance of the material, especially the mechanical performance, its ECM extraction material is also blended with one or more than two of the following components: polylactic acid, chitosan, ...

Embodiment approach 3

[0068] The following embodiments lie in successfully spinning two or more ECM extraction materials through electrospinning to obtain biomimetic tissue engineering scaffolds. It includes the following steps:

[0069] S1 Adding two or more ECM extraction materials to cross-linking agents respectively to obtain cross-linked materials after in-situ cross-linking reaction;

[0070] S2 Set the parameters of electrospinning and adjust the corresponding spinning orientation. According to the structure designed by the bionic tissue engineering scaffold, one or more cross-linked materials obtained are spun sequentially in sequence to obtain the bionic tissue engineering scaffold.

[0071] The electrospinning machine used may be a computer-controlled multi-nozzle electrospinning machine whose parameters can be set. It can also be a computer-controlled single-nozzle electrospinning machine with parameters that can be set. When it is necessary to spin a variety of ECM extraction materials...

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Abstract

The invention relates to a tissue engineering scaffold, in particular to a bionic tissue engineering scaffold and a preparation method thereof. The bionic tissue engineering scaffold comprises more than 90% by weight of a tissue-specific extracellular matrix (ECM) extraction material, wherein the ECM extraction material is extracted from biological tissues. The preparation method of the bionic tissue engineering scaffold comprises the following steps: adding more than one ECM extraction material into a cross-linking agent, carrying out an in-situ cross-linking reaction to generate a cross-linking material, and carrying out electrostatic spinning on the cross-linking material to prepare the bionic tissue engineering scaffold. The natural ECM extraction material is adopted as the main material of the bionic tissue engineering scaffold, and can provide enough mechanical strength and stability, so that by-products generated in the degradation process of artificially synthesized polymer materials are avoided, and biocompatibility is effectively improved. In addition, the tissue-specific ECM material provides an appropriate microenvironment and signal transduction for cell adhesion, proliferation and differentiation, so that regeneration and repair of complex tissues and organs can be promoted.

Description

technical field [0001] The invention relates to a tissue engineering support, in particular to a bionic tissue engineering support and a preparation method thereof. Background technique [0002] Tissue engineering scaffolds can mimic the physical and chemical properties of ECM to provide suitable conditions for cell adhesion and growth. At present, electrospinning technology is widely used in the preparation of tissue engineering scaffolds that imitate the structure of extracellular matrix (ECM). The disadvantages of this technology are as follows: [0003] 1. The disadvantages of electrospinning technology in terms of materials are: most of the electrospinning materials are artificially synthesized polymer materials, and the materials mainly include polyglycolide, polylactide and their copolymers. Due to the hydrophobicity of these polymer materials, it is not conducive to the adhesion of cells; the degradation products of some polymer materials are acidic, which is toxic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/54D01D5/00D04H1/728D04H3/02D04H13/00
CPCA61L27/3633A61L27/50A61L27/54D01D5/0084D04H1/728D04H3/02D04H13/00A61L2430/16A61L2400/12A61L2300/404A61L2300/416A61L2300/602
Inventor 林哲张进
Owner YIGONG RUISI FUJIAN ENG RES CENT CO LTD
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