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Preparation method of photoelectrochemical immunosensor for detecting prostate specific antigen

A prostate-specific, immunosensor technology, applied in the preparation of prostate-specific antigen detection and photoelectrochemical immunosensor fields, can solve the problems of low system sensitivity, reduced photoelectric conversion efficiency, etc., achieve high selectivity, increase photocurrent intensity, improve The effect of detection sensitivity

Active Publication Date: 2020-09-04
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the photocathode system mainly focuses on the dye-sensitized NiO semiconductor, and the rapid charge recombination between the holes in NiO and the photosensitizer reduces the photoelectric conversion efficiency and makes the system low sensitivity.

Method used

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  • Preparation method of photoelectrochemical immunosensor for detecting prostate specific antigen
  • Preparation method of photoelectrochemical immunosensor for detecting prostate specific antigen
  • Preparation method of photoelectrochemical immunosensor for detecting prostate specific antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Photosensitizer [Ir(C6) 2 (dcbpy)] + PF 6 - Synthesis and characterization of:

[0020] Weigh IrCl 3 ·3H 2 O solid (0.34g, 1mmol) was dissolved in water (5mL), stirred to fully dissolve, and coumarin-6 (0.77g, 2.2mmol) and 2-ethoxyethanol (15mL) were added to a 50mL round bottom flask refluxed at 120°C for 24 hours under the protection of nitrogen, the reaction product was naturally cooled to room temperature, the filter cake solid was collected by filtration, and then washed with water and ethanol in sequence to obtain an iridium dichloride bridged dimer product. 2,2'-bipyridine-4,4'-dicarboxylic acid (0.047 g, 0.25 mmol) and 2-ethoxyethanol (25 mL) and the resulting dimer (0.175 g, 0.1 mmol) were added to 50 mL in a round bottom flask. After stirring to dissolve, the excess Na 2 CO 3 (5mmol) was added to the above solution, stirred and heated, and refluxed under nitrogen protection for 20 hours. After the reaction was completed, the solvent was removed by di...

Embodiment 2

[0022] Preparation of the sensing working electrode:

[0023] (1) Preparation of NiO-modified ITO electrode: Put an ITO electrode (0.5cm×4.0cm) with a fixed area into 10mL ultrapure water, 10mL absolute ethanol, 10mL acetone, 10mL absolute ethanol, 10mL ultrapure water , ultrasonically cleaned for 10 minutes to remove impurities on the surface of the ITO electrode, and dried naturally. NiO nanofilms were modified on ITO electrodes by hydrothermal method. Dip the cleaned ITO glass into the configuration containing 400 μL of 0.25M Ni(NO 3 ) 2 ·6H 2O and 0.25M hexamethylenetetramine aqueous solution in a centrifuge tube, heated at 90°C for 200 minutes, cooled to room temperature, washed with ultrapure water three times, and dried under nitrogen atmosphere. Then place the ITO electrode in a muffle furnace at 350°C and keep it for 30 minutes, the heating rate is 5°C / min, and the temperature is naturally lowered to obtain a NiO-modified ITO electrode (ITO / NiO). Then it is immers...

Embodiment 3

[0025] Determination of samples:

[0026] The prepared sensing working electrode was immersed in different concentrations of PSA solutions, incubated at 37°C for 1 hour, washed and then detected by photocurrent, and the photocurrent intensity was recorded by a photoelectrochemical analyzer. The photocurrent detection adopts a three-electrode system: the prepared sensing working electrode, the platinum wire electrode as the auxiliary electrode, the Ag / AgCl electrode as the reference electrode, and the 0.1M PBS buffer solution (pH=7.4) as the detection solution under the excitation light of 475nm , the bias voltage is 0V, and the light source is turned on and off for 20s. When the PSA concentration is in the range of 1pg / mL to 10ng / mL, the photocurrent signal and the logarithm of its concentration have a good linear relationship, and the linear regression equation is ΔI=-720.151+53.800lg C PSA (pg / mL), linear correlation coefficient R 2 It was 0.9966, and the detection limit w...

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Abstract

The invention discloses a preparation method of a cathode photoelectric immunosensor of cyclometalated iridium complex sensitized NiO for detecting a prostate specific antigen. The preparation methodcomprises the following steps: firstly, preparing a nano porous NiO film on an ITO electrode by adopting a hydrothermal synthesis method; then fixing a photosensitizer [Ir(C6)2(dcbpy)]<+>PF6<-> (wherein C6 is coumarin 6 and dcbpy is 2,2'-dipyridine-4,4'-dicarboxylic acid) on the surface of the nano NiO film through a condensation effect; and the prostate specific antigen capture antibody is covalently modified on the electrode by virtue of chitosan and glutaraldehyde to prepare the sensing working electrode. Through the specific immune reaction between the prostate specific antigen and the antibody thereof, the detection of the concentration of the prostate specific antigen is realized, and the kit has high sensitivity and high specificity.

Description

technical field [0001] The invention belongs to the field of photoelectric functional materials and biological analysis, in particular to a method for preparing a photoelectrochemical immune sensor based on ring metal iridium complex sensitized NiO for detecting prostate specific antigen. Background technique [0002] Prostate-specific antigen (PSA), a serine protease secreted by prostate epithelial cells, is currently the most widely used biomarker for prostate cancer. Prostate cancer is one of the malignant tumors that seriously endanger men's health. In recent years, the incidence rate in my country has shown an obvious upward trend. Its early detection, diagnosis and treatment become the key to improve the survival rate and prognosis of patients. Prostate-specific antigen is increasingly used for early screening of prostate cancer so that early, asymptomatic prostate cancer can be detected. Therefore, it is of great significance to develop a detection method with high ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/573G01N27/327G01N27/48
CPCG01N33/57484G01N33/57434G01N33/573G01N27/3278G01N27/48G01N2333/96433
Inventor 李春香朱明王欢张迎涛
Owner QINGDAO UNIV OF SCI & TECH
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