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In-vitro diagnostic kit for determining activated partial thromboplastin time

A technology of thromboplastin time and in vitro diagnosis, which is applied in biological testing, material inspection products, etc., can solve the problems of widely different test results, different measurement results, and large differences between reagent bottles, etc., and achieves low production cost and convenient operation. , The effect of simple production equipment

Active Publication Date: 2020-09-08
SHENZHEN GOLDSITE DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality of APTT detection reagents currently on the market varies greatly over time, resulting in large differences in test results at different time periods within the validity period; There is a big difference between

Method used

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  • In-vitro diagnostic kit for determining activated partial thromboplastin time
  • In-vitro diagnostic kit for determining activated partial thromboplastin time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] An in vitro diagnostic kit for measuring activated partial thromboplastin time, comprising an APTT detection reagent and a plastic bottle for holding the APTT detection reagent; the APTT detection reagent consists of a partial thromboplastin reagent and a calcium salt solution Composition, the part of the thromboplastin reagent is prepared from cephalin solution and buffer, and the buffer is made of the following raw materials in weight percentage: activator 0.5%, tris 3%, sodium chloride 5%, manna Alcohol 2%, polyethylene glycol 8%, bovine serum albumin 5%, alanine 6%, carbolic acid 1%, proclin300 0.8%, and the balance is water.

[0060] The calcium salt solution is a calcium chloride solution, and the concentration of calcium chloride in the calcium chloride solution is 25mmol / L.

[0061] The cephalin is rabbit brain cephalin; the activator is ellagic acid; and the polymerization degree of polyethylene glycol is 6000.

[0062] The preparation method of described part...

Embodiment 2

[0067] An in vitro diagnostic kit for measuring activated partial thromboplastin time, comprising an APTT detection reagent and a plastic bottle for holding the APTT detection reagent; the APTT detection reagent consists of a partial thromboplastin reagent and a calcium salt solution Composition, said partial thromboplastin reagent is prepared by cephalin solution and buffer, and said buffer is made of the following raw materials in weight percentage: activator 0.3%, tris 0.1%, sodium chloride 0.1%, mannose Alcohol 1%, polyethylene glycol 1%, bovine serum albumin 1%, alanine 0.5%, carbolic acid 0.1%, proclin300 0.5%, and the balance is water.

[0068] The calcium salt solution is a calcium chloride solution, and the concentration of calcium chloride in the calcium chloride solution is 15mmol / L; the cephalin is bovine brain cephalin; the activator is kaolin; the polyethylene The degree of polymerization of the diol was 4,000.

[0069] The preparation method of described partia...

Embodiment 3

[0074] An in vitro diagnostic kit for measuring activated partial thromboplastin time, comprising an APTT detection reagent and a plastic bottle for holding the APTT detection reagent; the APTT detection reagent consists of a partial thromboplastin reagent and a calcium salt solution Composition, said partial thromboplastin reagent is prepared by cephalin solution and buffer, and said buffer is made of the following raw materials in percentage by weight: activator 1.0%, tris 8%, sodium chloride 10%, manna Alcohol 3%, polyethylene glycol 20%, bovine serum albumin 10%, alanine 10%, carbolic acid 2%, proclin300 1%, and the balance is water.

[0075] Described calcium salt solution is calcium chloride solution, and the concentration of calcium chloride in described calcium chloride solution is 30mmol / L; Described cephalin is rabbit brain cephalin; Described activator is diatomaceous earth; The degree of polymerization of ethylene glycol is 8000.

[0076] The preparation method of...

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Abstract

The invention discloses an in-vitro diagnostic kit for determining activated partial thromboplastin time. The in-vitro diagnostic kit comprises an APTT detection reagent and a plastic bottle for containing the APTT detection reagent, the APTT detection reagent is composed of a partial thromboplastin reagent and a calcium salt solution, and the partial thromboplastin reagent is prepared from a cephalin solution and a buffer solution, wherein the buffer solution is prepared from the following raw materials: an activating agent, tris, sodium chloride, mannitol, polyethylene glycol, bovine serum albumin, alanine, carbolic acid, proclin 300 and water. The in-vitro diagnostic kit for determining activated partial thromboplastin time is convenient to use, improves the stability of an APTT detection reagent on the basis of ensuring the accuracy and sensitivity of the APTT detection reagent, enables the in-vitro diagnostic kit to be widely applied clinically, and enables APTT detection to be standardized.

Description

technical field [0001] The invention relates to the field of clinical diagnostic reagents, in particular to an in vitro diagnostic kit for measuring activated partial thromboplastin time. Background technique [0002] Whether the blood coagulation function is normal or not is related to the physiological health of the human body, and the evaluation experiment of blood coagulation function is of great significance to the diagnosis, treatment and prognosis of various diseases. The blood coagulation process is complex and the detection indicators are diverse, which are mainly divided into extrinsic coagulation pathway and intrinsic coagulation pathway. Coagulation time is an important indicator to measure the state of coagulation, and it is of great significance for judging the pathological changes of the body's hemostasis and coagulation system, and for preoperative screening of coagulation diseases. Among them, activated partial thromboplastin time (Activated partial thrombo...

Claims

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Application Information

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IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 郑琳陈明峰郑筱雯余嘉陵
Owner SHENZHEN GOLDSITE DIAGNOSTICS