A kind of antioxidant peptide and its preparation method and application

An anti-oxidative peptide and amino acid technology, applied in the biological field, can solve the problems of less research on functional characteristics, and achieve the effect of high-value utilization, strong practicability, and significant anti-oxidative protection

Active Publication Date: 2022-05-20
TIANJIN UNIV OF COMMERCE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peptides with good antioxidant activity have been obtained from plant protein (such as soybean protein and oilseed protein), milk protein, and marine organisms, but studies based on antioxidant peptides derived from meat protein and their functional properties are relatively limited. few

Method used

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  • A kind of antioxidant peptide and its preparation method and application
  • A kind of antioxidant peptide and its preparation method and application
  • A kind of antioxidant peptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of antioxidant peptides:

[0033] 1-1. Extraction of chicken breast myofibrillar protein:

[0034] Remove visible adipose connective tissue from the chicken breast, chop it into minced meat with a knife, add four times the volume of protein extract to homogenize for 25 seconds, centrifuge at 5000r / min for 15 minutes at 4°C, discard the supernatant and save the precipitate. Add protein extract and repeat the above operation twice to obtain crude protein. The composition of protein extraction solution used is: the concentration of NaCl is 0.1mol / L, MgCl 2 The concentration of 0.002mol / L, the concentration of EDTA·2Na is 0.001mol / L, NaH 2 PO 4 / Na 2 HPO 4 The concentration is 0.1mol / L, and the pH value is 7.0.

[0035] The obtained crude protein was mixed with 4 times the volume concentration of 0.1 mol / L NaCl solution, homogenized for 25 s, centrifuged at 5000 r / min for 15 min at 4 °C, and the supernatant was discarded to save the precipitate. Then a...

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Abstract

The invention discloses an antioxidant peptide, its preparation method and application, and aims to provide an antioxidant peptide with high-efficiency antioxidant activity derived from chicken breast myofibrillar protein, its preparation method and application. The amino acid sequence of the antioxidant peptide of the present invention is ITTNPYDY or IGWSPLGSL. The preparation method of the antioxidant peptide comprises the following steps: extracting myofibrillar protein from fresh chicken breast meat, enzymatically hydrolyzing the obtained myofibrillar protein with pepsin, and obtaining the antioxidant peptide through separation, purification and synthesis techniques. The antioxidant peptide of the present invention is a new type of antioxidant peptide, which has strong antioxidant activity after testing, and its iron ion reduction ability and oxygen free radical absorption ability are significantly higher than those of glutathione under the measurement conditions of the present invention , and at the same time, it has a significant inhibitory effect on the oxidative damage of NIH‑3T3 cells. The antioxidant peptide of the invention belongs to natural antioxidants, is safer to eat, and can be applied to antioxidant functional foods, health care products and medicines.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to an antioxidant peptide derived from chicken breast myofibrillar protein and its preparation method and application. Background technique [0002] Excessive reactive oxygen species (ROS) free radicals will destroy the normal redox balance of the body, causing oxidative damage to intracellular biomacromolecules such as proteins, lipids, DNA, etc., thereby accelerating the aging of the body, causing neurodegenerative diseases and atherosclerosis , chronic inflammation, cancer and many other diseases. Studies have found that dietary intake of antioxidants to remove excessive free radicals in the body and reduce the level of oxidative stress in the body is an effective method, which can prevent and even treat certain diseases. At present, some synthetic antioxidants, such as butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), tert-butylhydroquinone (TBHQ), etc., are wid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K14/465C07K1/36C07K1/34C07K1/16C07K1/04C12P21/06A23L33/18A61P39/06
CPCC07K14/78C07K14/465C12P21/06A23L33/18A61P39/06A23V2002/00A23V2200/30
Inventor 陈金玉曲金萍张坤生闫怡君张蕾蕾郑嘉玉
Owner TIANJIN UNIV OF COMMERCE
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