Bifidobacterium animalis NX-6 and application thereof in preparation of lipid-lowering and weight-losing medicines

An animal bifidobacteria, NX-6 technology, applied in the field of microorganisms, can solve the problems of insufficient development of probiotics' blood lipid-lowering function, imperfect functional evaluation path, and few probiotics, etc., to prevent or treat cardiovascular diseases, Good weight and length gain, the effect of improving body mass index

Active Publication Date: 2020-09-18
广东南芯医疗科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research on probiotics in China is only focused on basic research, and the development of probiotics’ blood lipid-lowering function is not enough, and the function evaluation path is not perfect, which eventually leads to the scarcity of excellent strains with independent intellectual property rights in my country, which are used clinically to replace or There are very few functional drugs and probiotics for adjuvant therapy

Method used

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  • Bifidobacterium animalis NX-6 and application thereof in preparation of lipid-lowering and weight-losing medicines
  • Bifidobacterium animalis NX-6 and application thereof in preparation of lipid-lowering and weight-losing medicines
  • Bifidobacterium animalis NX-6 and application thereof in preparation of lipid-lowering and weight-losing medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation, Identification and Preservation of Novel Animal Bifidobacterium NX-6

[0028] (1) Isolation: After the fecal samples were serially diluted, they were respectively inoculated in BS solid medium, cultured anaerobically at 37°C for 48h-72h, and the single colony on the plate was picked and streaked for three generations to obtain the purified strain. Inoculate the single clone obtained by streaking the third generation into BS liquid medium, culture anaerobically at 37°C for 12-16h, add 40% glycerol, mix the bacterial liquid and glycerol in equal volumes, and store in a -80°C refrigerator.

[0029] (2) Morphological identification of strains: Enzyme contact test was carried out on the screened strains to observe whether there were bubbles. Gram-stained strains were checked with an oil microscope, Gram-positive strains were purple, and Gram-negative strains were red. Bifidobacteria can be observed in Y-type and V-type characteristics.

[0030] (3) Rap...

Embodiment 2

[0037] The preparation of embodiment 2 novel bifidobacterium animalis NX-6 fermentation supernatant (extracellular secretion), bacterium suspension (thalline) and cell disruptor supernatant (intracellular substance)

[0038] The new type of bifidobacterium animalis NX-6 was activated and cultured and inoculated in BS liquid medium. After culturing at 37°C for 15 hours, the concentration of fermentation bacteria was adjusted to 10 9 CFU / mL, centrifuged at 4°C, 6000r / min for 10min to obtain the culture supernatant and cell pellet, the supernatant was filtered through a 0.22μm filter membrane to obtain the fermentation supernatant (extracellular secretion); the cell pellet was filtered through PBS twice After the first wash, the cells were resuspended with PBS, and the cell concentration was adjusted to 10 9 CFU / mL to obtain bacterial suspension (bacteria); process the bacterial suspension in an ice bath with an ultrasonic breaker, work for 3s, interval 8s, ultrasonic break for 1...

Embodiment 3

[0039] Embodiment 3 zebrafish rearing

[0040] Lovastatin was purchased from Shanghai Luyuan Biotechnology Co., Ltd., and common feed and high-cholesterol feed were purchased from Nantong Trophy Feed Technology Co., Ltd.

[0041] Under the microscope, the normal wild-type AB line zebrafish (5dpf) was selected and divided into normal group (blank control group), model group (high cholesterol), positive control group (lovastatin), samples (not inactivated and inactivated: Bacterial suspension, fermentation supernatant, cell crush supernatant) intervention group, 200 pieces in each group. The zebrafish in the normal group were fed with common feed (40 mg each time); the zebrafish in the model group were fed with a high-cholesterol feed (40 mg each); the zebrafish in the positive control group were fed with a high-cholesterol feed+lovastatin (high cholesterol feed and lovastatin) Statin was mixed in a weight ratio of 19:1, 40 mg each time); the zebrafish in the sample interventio...

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Abstract

The invention discloses bifidobacterium animalis NX-6 and application thereof in preparation of lipid-lowering and weight-losing medicines, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium animalis NX-6 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 20114. The uninactivated and inactivated fermentation supernate, bacterial suspension and cell disrupted supernate of novel bifidobacterium animalis NX-6 show good effects of slowing down weight and body length increase, improving body mass index(BMI) and reducing fat area percentage on zebrafish, and have a potential weight losing effect; triglyceride (TG) and total cholesterol (TC) in a zebrafish hyperlipidemia model body can be obviously reduced, and a good hypolipidemic effect is shown. The novel bifidobacterium animalis NX-6 disclosed by the invention has a huge potential application prospect in the aspect of preparing medicines fortreating and/or preventing obesity and hyperlipidemia.

Description

technical field [0001] The invention relates to the technical field of microbes, and more specifically relates to an animal bifidobacterium NX-6 and its application in the preparation of lipid-lowering and weight-loss drugs. Background technique [0002] Hyperlipidemia is a sub-health or disease state in which blood lipids exceed normal levels. It is the main cause of cardiovascular diseases such as coronary heart disease, atherosclerosis, and hypertension. relevant. From 2002 to 2012, the prevalence of dyslipidemia among adults increased substantially, and the treatment and control rates were low. The results of the 2013-2014 China Chronic Disease and Risk Factor Surveillance Survey showed that the serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and high-density lipoprotein cholesterol in Chinese population aged ≥18 years (HDL-C) were 4.70mmol / L, 2.88mmol / L, 1.14mmol / L, 1.35mmol / L, and the TC level was significantly h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/04A61P3/06C12R1/01
CPCA61K35/745A61P3/04A61P3/06C12R2001/01C12N1/205
Inventor 张召郑康帝赵笑笑马燕陈涛
Owner 广东南芯医疗科技有限公司
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