Syngnathus sterol solid dispersion and application thereof in treatment of stroke
A technology of solid dispersion and dragon sterol, which is applied in the field of biomedicine, can solve the problems of decreased peripheral immunosuppression and poor drug safety, and achieves the effect of simple preparation method, low toxicity and high safety
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[0037] The preparation method of the basic methanol aqueous solution is as follows: methanol and water are mixed according to the volume ratio of 1:0.25, and 10wt% sodium hydroxide is added to obtain the product.
[0038] Determination method of the purity of nigrosterol (based on cholesterol content):
[0039] (1) Standard curve preparation: Accurately weigh cholesterol and dissolve it in n-hexane to make standard products with different concentrations; accurately measure 2ml of acetic anhydride in a 10ml test tube with a stopper, add 1 drop of concentrated sulfuric acid dropwise, and immediately add each Concentration standard solution 1ml, vortex and shake well, let it stand for 10min, and measure the absorbance of each concentration standard solution at a wavelength of 650nm. Take the concentration of cholesterol solution as the abscissa, and use the OD value corresponding to the cholesterol solution as the ordinate to draw a standard curve and calculate the regression equ...
Embodiment 1
[0042] Embodiment 1 a kind of dragon sterol solid dispersion
[0043] The preparation of described picrosterol solid dispersion specifically comprises the following steps:
[0044] S1. Superfinely pulverize 1 kg of dried sea dragon product, add 5 L of alkaline methanol aqueous solution, heat and reflux for 8 hours, cool to normal temperature and filter to obtain the filtrate;
[0045]S2. Add saturated calcium chloride solution dropwise to the filtrate obtained in step S2, and stir while adding dropwise until there is no precipitation, leave it for 12 hours, and filter to obtain a precipitate;
[0046] S3, add 800ml of acetone to the precipitate, heat to reflux for 4h, filter, concentrate the filtrate to 30% of the original volume, cool and crystallize, wash the crystal with 300ml of ethanol at 4°C, collect the crystal by filtration, and dry to obtain 23.5g of sterol extract. The measured purity of sterol is 93%;
[0047] S4. Dissolve 5 g of the pentagon sterol extract obtain...
Embodiment 2
[0048] Embodiment 2 a kind of dragon sterol solid dispersion
[0049] The preparation of described picrosterol solid dispersion specifically comprises the following steps:
[0050] S1. Superfinely pulverize 5 kg of dried sea dragon product, add 35 L of alkaline methanol aqueous solution, heat and reflux for 8 hours, cool down to normal temperature and filter to obtain the filtrate;
[0051] S2. Add saturated calcium chloride solution dropwise to the filtrate obtained in step S2, and stir while adding dropwise until there is no precipitation, leave it for 12 hours, and filter to obtain a precipitate;
[0052] S3, add 2000ml of acetone to the precipitate, heat to reflux for 6h, filter, concentrate the filtrate to 10% of the original volume, cool and crystallize, wash the crystal with 400ml of ethanol at 4°C, collect the crystal by filtration, and dry to obtain 112.5g of sterol extract. The measured purity of sterol is 92.6%;
[0053] S4. Dissolve 10 g of the sterosterol extrac...
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