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DC-CIK cell preparation and preparation method thereof

A technology of DC-CIK and cell preparations, applied in the direction of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems of no unified conclusion, achieve low cost, high cytotoxicity, and good use effect

Inactive Publication Date: 2020-09-22
海南优尼科尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, antiviral drugs are often used to control the further development of the disease, but there is still no unified clinical conclusion as to which drug is more beneficial to improve the therapeutic effect and liver function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of DC-CIK cell preparation, comprises DC-CIK cell, 20% human albumin and solvent, and the concentration of described DC-CIK cell is every milliliter 0.5 * 10 7 cells, the content of the 20% human albumin is 1% by volume, the solvent is 120ml of 0.9% saline, and the DC-CIK cells are mononuclear cells separated from peripheral blood of autologous origin DC cells and CIK cells were respectively induced and cultured, and then co-cultured to obtain. The DC cells were added with granulocyte-macrophage colony-stimulating growth factor, interleukin-4 and HBsAg during the culture process; the CIK cells were cultured during the culture process. Anti-CD3 monoclonal antibody, γ-interferon, interleukin-2 and autologous plasma were added to the DC-CIK cells, interleukin-2 and autologous plasma were added during the culture process of the DC-CIK cells, and the medium was added with 80,000 units Gentamicin GT-T551 basal medium, the DC cells were cultured in T75 culture flasks, a...

Embodiment 2

[0039] A kind of DC-CIK cell preparation, comprises DC-CIK cell, 20% human albumin and solvent, and the concentration of described DC-CIK cell is every milliliter 2 * 10 7 The content of the 20% human serum albumin is 4% by volume, the solvent is 200ml of 0.9% saline, and the DC-CIK cells are mononuclear cells separated from peripheral blood of autologous origin DC cells and CIK cells were respectively induced and cultured, and then co-cultured to obtain. The DC cells were added with granulocyte-macrophage colony-stimulating growth factor, interleukin-4 and HBsAg during the culture process; the CIK cells were cultured during the culture process. Anti-CD3 monoclonal antibody, γ-interferon, interleukin-2 and autologous plasma were added to the DC-CIK cells, interleukin-2 and autologous plasma were added during the culture process of the DC-CIK cells, and the medium was added with 80,000 units Gentamicin GT-T551 basal medium, the DC cells were cultured in T75 culture flasks, and ...

Embodiment 3

[0049] A kind of DC-CIK cell preparation, comprises DC-CIK cell, 20% human albumin and solvent, and the concentration of described DC-CIK cell is every milliliter 1.2 * 10 7 The content of the 20% human serum albumin is 2.5% by volume, the solvent is 160ml of 0.9% normal saline, and the DC-CIK cells are mononuclear cells separated from autologous peripheral blood DC cells and CIK cells were respectively induced and cultured, and then co-cultured to obtain. The DC cells were added with granulocyte-macrophage colony-stimulating growth factor, interleukin-4 and HBsAg during the culture process; the CIK cells were cultured during the culture process. Anti-CD3 monoclonal antibody, γ-interferon, interleukin-2 and autologous plasma were added to the DC-CIK cells, interleukin-2 and autologous plasma were added during the culture process of the DC-CIK cells, and the medium was added with 80,000 units Gentamicin GT-T551 basal medium, the DC cells were cultured in T75 culture flasks, and...

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PUM

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Abstract

The invention relates to a DC-CIK cell preparation and a preparation method thereof. The DC-CIK cell preparation comprises DC-CIK cells, 20% human serum albumin and a solvent. The preparation method comprises the following preparation steps: 1) collecting peripheral blood into a blood collection bag containing a heparin sodium anticoagulant; 2) adding isopyknic normal saline into a lower-layer redliquid after peripheral blood centrifugation, and carrying out diluting, blowing, beating and uniformly mixing; 3) resuspending and inoculating the peripheral blood mononuclear cells into a T75 culture bottle containing a 5 ml culture medium by using a 10 ml culture medium, and performing culturing in a 5% CO2 incubator at 37 DEG C for 2-6 h; 4) inducting and amplifying DC cells in vitro; 5) inducting and amplifying CIK cells in vitro; 6) co-culturing DC cells and CIK cells; and 7) preparing the DC-CIK cell preparation. The DC-CIK cell preparation has the advantages of high proliferation activity, large cell factor release amount, high cytotoxicity, simplicity, feasibility, low cost and good use effect.

Description

technical field [0001] The invention relates to a DC-CIK cell preparation and a preparation method thereof. Background technique [0002] Hepatitis B (CHB) is an infectious disease caused by HBV infection, which mainly damages the liver cells of the human body, causing fibrosis, inflammatory reactions, necrosis and liver cirrhosis in the liver cells. Clinically, antiviral drugs are often used to control the further development of the disease, but there is still no unified clinical conclusion as to which drug is more beneficial to improve the therapeutic effect and liver function. With the advancement of science and technology, immunotherapy has become the most important and promising treatment method besides surgery, chemotherapy and radiotherapy. [0003] Dendritic cells (DC cells) are the most powerful antigen-presenting cells (Antigen presenting cells, APCs) in the body, which can stimulate the proliferation of initial T lymphocytes and initiate immune responses; they ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/0783A61K35/15A61K35/17A61P31/20A61P1/16
CPCA61K35/15A61K35/17A61P1/16A61P31/20C12N5/0638C12N5/0639C12N2500/80C12N2501/22C12N2501/2302C12N2501/2304C12N2501/24C12N2501/515C12N2501/998C12N2502/00A61K2300/00
Inventor 王晓宇李崴郭康合李翠云林冠妃
Owner 海南优尼科尔生物科技有限公司
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