A Microscopic Imaging Method to Obtain Double Contrast of Light Absorption and Light Scattering Simultaneously

A microscopic imaging, dual-contrast technology, applied in scattering property measurement, color/spectral property measurement, material analysis by optical means, etc., can solve the problem that it is difficult to achieve clear imaging with optical microscope
CN111693465BActive Publication Date: 2021-06-22NANJING UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2021-06-22

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Abstract

The invention discloses a microscopic imaging method for obtaining double contrast of light absorption and light scattering at the same time. The pulsed laser is focused on the sample, and the sample absorbs part of the pulsed laser, and the ultrasonic signal is excited due to the photoacoustic effect. 1. The ultrasonic transducer arranged coaxially receives the ultrasonic signal and obtains an image with light absorption contrast; at the same time, the remaining pulsed laser light that passes through the sample is scattered and absorbed by the sample, and then irradiates on the same surface of the ultrasonic transducer, and then Another ultrasound signal is excited and received by the ultrasound transducer, from which an image with light scattering contrast can be obtained. For any imaging point on the sample, the present invention can realize light scattering-light absorption dual-mode microscopic imaging at the same time with only one optical excitation; moreover, this method is a high-resolution imaging technology without exogenous labeling, avoiding The possible influence of the marker on the properties of the sample is understood, and it has high ease of use and practicability.
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Description

technical field

[0001] The invention relates to a microscopic imaging method capable of simultaneously obtaining double-contrast ratios of light absorption and light scattering. Through one scan, microscopic images of two contrast ratios of sample light absorption and light scattering can be obtained simultaneously. Background technique

[0002] For transparent substances, such as most transparent cells, histopathological sections, etc., when the light intensity changes slightly after passing through these substances, it is difficult to achieve clear imaging with ordinary optical microscopes. Therefore, staining is often required for imaging transparent substances such as living cells and histopathological sections, and label-free imaging is still an important challenge for the imaging of cells and histopathological sections.

[0003] Light scattering is an effective imaging parameter for characterizing transparent substances such as cells. By measuring the intensity, phase...

Claims

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