Vertical graphene glucolase working electrode, preparation method and biosensor

A technology of glucose enzyme and working electrode, applied in metal processing equipment, nanotechnology for sensing, instruments, etc., can solve the problems of continuous monitoring stability and sensitivity to be improved, needles that cannot be used for a long time, and untimely diagnosis, etc. Achieve the effect of facilitating the transfer of electrons, easy access, and low detection limit

Pending Publication Date: 2020-09-25
SHENZHEN YICK XIN TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current blood glucose monitoring devices mainly use screen-printed thick-film electrode test strips for fingertip blood testing, which brings inconvenience and pain to patients, and the diagnosis is not timely
Although some international advanced enterprises have developed needle-type glucose biosensor electrodes, the miniaturization, continuous monitoring stability and sensitivity of the existing glucose biosensor electrodes still need to be improved
The current needles inserted into the body cannot be used for a long time and bring pain to the patient

Method used

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  • Vertical graphene glucolase working electrode, preparation method and biosensor
  • Vertical graphene glucolase working electrode, preparation method and biosensor
  • Vertical graphene glucolase working electrode, preparation method and biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A biosensor comprising an upright graphene glucose enzyme working electrode.

[0064] Described upright graphene glucose enzyme working electrode, such as image 3 As shown, it includes a vertical few-layer graphene film 1, a glucose oxidase layer 2 coated on the surface of the vertical few-layer graphene film 1, and a porous hydrophilic protective functional film 3 coated on the surface of the glucose oxidase layer 2, The vertical few-layer graphene film 1 is connected with non-hexacyclic large pi bonded carbon atom point defect clusters (not shown in the figure) and platinum nanoparticles 4 .

[0065] The preparation method of described upright graphene glucose enzyme working electrode, it comprises the following steps:

[0066] Step 1, using plasma-enhanced chemical vapor deposition method to grow vertical few-layer graphene film on the surface of conductive substrate;

[0067] Step 2, using a plasma method to form non-hexacyclic large pi bonded carbon atom point d...

Embodiment 2

[0096] A biosensor comprising an upright graphene glucose enzyme working electrode.

[0097] The structure of the vertical graphene glucose enzyme working electrode is the same as that of Example 1.

[0098] The preparation method of described upright graphene glucose enzyme working electrode, it comprises the following steps:

[0099] Step 1, using plasma-enhanced chemical vapor deposition method to grow vertical few-layer graphene film on the surface of conductive substrate;

[0100] Step 2, adopting a plasma method to form hydroxyl, carboxyl and amino groups on the surface of the vertical few-layer graphene film;

[0101] Step 3, using the magnetron plasma sputtering source physical vapor deposition method to deposit Pt nanoparticles on the surface of the vertical few-layer graphene film treated in step 2;

[0102] Step 4, prepare the glucose oxidase mixed solution, drop the glucose oxidase mixed solution onto the surface of the vertical few-layer graphene film treated in...

Embodiment 3

[0130] A biosensor comprising an upright graphene glucose enzyme working electrode.

[0131] The structure of the vertical graphene glucose enzyme working electrode is the same as that of Example 1.

[0132] The preparation method of described upright graphene glucose enzyme working electrode, it comprises the following steps:

[0133] Step 1, using plasma-enhanced chemical vapor deposition method to grow vertical few-layer graphene film on the surface of conductive substrate;

[0134] Step 2, adopting a plasma method to form hydroxyl, carboxyl and amino groups on the surface of the vertical few-layer graphene film;

[0135] Step 3, using the magnetron plasma sputtering source physical vapor deposition method to deposit Pt nanoparticles on the surface of the vertical few-layer graphene film treated in step 2;

[0136] Step 4, prepare the glucose oxidase mixed solution, drop the glucose oxidase mixed solution onto the surface of the vertical few-layer graphene film treated in...

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Abstract

The invention relates to the technical field of preparation and application of nano materials. The invention particularly relates to a vertical graphene glucolase working electrode, a preparation method and a biosensor. The vertical graphene glucolase working electrode comprises a vertical few-layer graphene film, a glucose oxidase layer coating the surface of the vertical few-layer graphene film,and a porous hydrophilic protection functional film coating the surface of the glucose oxidase layer, wherein the vertical few-layer graphene film is connected with a non-hexacyclic large pi bonded carbon atom point defect cluster and platinum nanoparticles. The vertical graphene glucolase working electrode can be used for continuous painless detection of glucose, and has extremely high detectionsensitivity.

Description

technical field [0001] The invention relates to the technical field of preparation and application of nanometer materials, in particular to an upright graphene glucose enzyme working electrode, a preparation method and a biosensor. Background technique [0002] Diabetes is a disease caused by insufficient secretion of insulin by the pancreas or ineffective use of insulin. The concentration of glucose is one of the important indicators for the diagnosis and treatment of diabetes. Therefore, blood glucose monitoring devices are commonly used medical equipment in the treatment of diabetes. Continuous blood glucose monitoring equipment is currently a hot spot in the research and development of the industry. Current blood glucose monitoring devices mainly use screen-printed thick-film electrode test strips for fingertip blood testing, which brings inconvenience and pain to patients, and the diagnosis is not timely. Although some international advanced companies have developed ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30C23C28/00C23C16/50C23C16/26C23C14/35C23C14/18C23C14/16B22F1/00B82Y15/00B82Y30/00B82Y40/00
CPCG01N27/3278G01N27/308C23C28/322C23C28/34C23C16/50C23C16/26C23C14/35C23C14/185C23C14/165B82Y15/00B82Y30/00B82Y40/00B22F1/054
Inventor 石柳婷赵鑫钟西舟陈旺寿
Owner SHENZHEN YICK XIN TECH DEV CO LTD
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