Method for rapidly determining non-edible substance trithiocyanuric acid trisodium salt in wheat flour and additives thereof by high performance liquid chromatography

A technology of trisodium thiocyanate and high performance liquid chromatography, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficulty in extraction, unsuitable solvents, complex matrix, etc., and achieve simple and fast sample pretreatment. , quantitative and accurate effect

Active Publication Date: 2020-09-29
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Application Information

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Problems solved by technology

It is also easier to extract and purify the flour treatment agent with a simple matrix, while the additives based on edible cornstarch, amylase and other compound enzymes have a complex matrix and are not suitable for many solvents when they meet water and tend to gel. high difficulty

Method used

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  • Method for rapidly determining non-edible substance trithiocyanuric acid trisodium salt in wheat flour and additives thereof by high performance liquid chromatography
  • Method for rapidly determining non-edible substance trithiocyanuric acid trisodium salt in wheat flour and additives thereof by high performance liquid chromatography
  • Method for rapidly determining non-edible substance trithiocyanuric acid trisodium salt in wheat flour and additives thereof by high performance liquid chromatography

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Embodiment 1

[0024] 1 Experimental part

[0025] 1.1 Materials and reagents

[0026] Trisodium thiocyanate (TMT) standard (adamas-beta); Atlantis T3 chromatographic column (250 mm × 4.6 mm, 5 µm) Waters Company, USA; Methanol, acetonitrile (chromatographically pure) Fisher Company, USA; formic acid, Ammonium formate, diammonium hydrogen phosphate, ammonium acetate (analytical pure) Sinopharm Chemical Reagent Co., Ltd. The wheat flour and flour treatment agents used in the experiment were purchased from supermarkets.

[0027] 1.2 Instruments and equipment

[0028] Waters 2695 high performance liquid chromatograph (equipped with Waters 2998 diode array detector) American Waters company; AB204-S type electronic balance Swiss Mettler Toledo company; SB-800DTD type ultrasonic cleaner China Ningbo Xinzhi Biotechnology Co., Ltd.; 3- 18K refrigerated centrifuge from Sigma, Germany; Milli-Q ultrapure water preparation from MILLIPORE, USA; vortex mixer from IKA, Germany; PTFE microporous membrane...

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Abstract

The invention discloses a method for rapidly determining non-edible substance trithiocyanuric acid trisodium salt in wheat flour and additives thereof by high performance liquid chromatography. The method comprises the steps of performing vortex ultrasonic extraction on total trithiocyanuric acid trisodium salt in a sample by using an acetonitrile-formic acid / ammonium formate buffer solution system, performing gradient elution by using methanol-acidified ammonium acetate as a mobile phase, separating by using an Atlantis T3 chromatographic column, detecting by using a diode array detector, andquantifying by using an external standard method. The optimized acetonitrile-formic acid / ammonium formate buffer solution extraction system not only can efficiently extract different forms of TMT, but also can well disperse different matrix samples, and overcomes the problem that the wheat flour improver is easy to gelatinize in extraction. The method is simple, convenient, efficient, good in accuracy and suitable for rapid qualitative and quantitative analysis of the illegal additive TMT in the wheat flour and the treating agent of the wheat flour, and technical support is provided for guaranteeing the quality safety of the wheat flour.

Description

technical field [0001] The invention belongs to the field of food testing, and in particular relates to a method for measuring trisodium thiocyanate in wheat flour and its additives by high performance liquid chromatography. Background technique [0002] Trisodium tripolythiocyanate, also known as trimercapto-s-triazine trisodium salt, 1,3,5-triazine-2,4,6(1H,3H,5H)-trithiol trisodium salt, etc. It is a pure white crystal with the molecular formula C 3 N 3 S 3 Na 3 , with a molecular weight of 243.22, easily soluble in water. Trisodium thiocyanate (TMT) is a heavy metal ion remover, which has been used for the treatment of industrial wastewater containing heavy metal ions for many years. TMT was first produced by Degussa, which can interact with most monovalent and divalent heavy metal ions in aqueous solution, such as Cu 2+ , Pd 2+ , Hg 2+ , Zn 2+ 、Ni 2+ 、Ti 2+ 、Cd 2+ Plasma forms stable compounds and precipitates out, so as to achieve the purpose of removing he...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/74G01N2030/047
Inventor 刘艳明尹丽丽张艳侠薛霞祝建华胡梅赵慧男张喜琦武传香
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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