Pretreatment method of hot processed food and detection method of acrylamide
A technology of acrylamide and detection method, applied in the field of analysis and detection, can solve the problems of low detection accuracy, large interference of impurities and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0121] (1) Preparation of acrylamide series standard working solutions
[0122] (1.1) Prepare 1.0 mg / mL acrylamide standard stock solution
[0123] Accurately weigh 10 mg of acrylamide standard substance (accurate to 0.01 mg), dissolve it in methanol in a 10 mL brown volumetric flask, adjust the volume to the mark, and store in a sealed, dark place at -20°C.
[0124] (1.2) Prepare 10mg / L acrylamide standard stock solution
[0125] Accurately draw 0.25mL of 1.0mg / mL acrylamide standard stock solution into a 25mL brown volumetric flask, dilute to the mark with methanol, and store in a sealed seal at -20°C in the dark.
[0126] (1.3) Prepare 1.0mg / L acrylamide standard solution
[0127] Accurately draw 1.00 mL of 10 mg / L acrylamide standard stock solution into a 10 mL brown volumetric flask, and dilute to the mark with 0.1% formic acid aqueous solution. The 10 mg / L acrylamide standard stock solution is preferably ready-to-use.
[0128] (1.4) Prepare 0.5mg / mL 13 C 3 -Acrylamide...
Embodiment 2
[0144] (1) Sample pretreatment
[0145] (1.1) First crush the potato chips with a pulverizer, accurately weigh 0.5 g (accurate to 0.0001 g) of the crushed potato chips sample into a 25 mL graduated centrifuge tube, add 40 μL of 5.0 μg / L 13 C 3 -Acrylamide internal standard application solution and 10mL ultrapure water, ultrasonically extract for 10min, add 5mL of dichloromethane to degrease, vortex for 5min, centrifuge at 10000r / min for 3min, take out, and the supernatant is the extract;
[0146] (1.2) Put 5.0mL of the sample extraction solution into a GCB / PSA column for purification, wherein the flow rate of the sample extraction solution is 2mL / min, and collect the sample purification solution into a 15mL graduated centrifuge tube;
[0147] (1.3) Add 0.75g potassium bromide to 5mL sample purification solution and vortex to dissolve, add 0.3mL sulfuric acid solution with a concentration of 0.3mol / L, 0.5mL potassium bromate solution with a mass volume percent concentration of...
Embodiment 3
[0157] Detect according to the method of Example 2, the difference from Example 2 is: the concentration of acrylamide in the sample liquid to be tested is 100 μg / kg, and the test result is the average value of 6 experiments, and the test results are shown in Table 4 shown.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com