Poultry-derived characteristic collagen peptide and application thereof in detection of collagen hydrolysate and products thereof

A technology for sex glue and poultry, applied in specific peptides, biological tests, measuring devices, etc., can solve problems such as insurmountable difficulties, and achieve the effects of high accuracy, high sensitivity and strong characteristics

Pending Publication Date: 2020-10-02
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned difficulties cannot be overcome by directly using liquid mass spectrometry to detect and analyze samples.

Method used

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  • Poultry-derived characteristic collagen peptide and application thereof in detection of collagen hydrolysate and products thereof
  • Poultry-derived characteristic collagen peptide and application thereof in detection of collagen hydrolysate and products thereof
  • Poultry-derived characteristic collagen peptide and application thereof in detection of collagen hydrolysate and products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1 Materials and Reagents

[0023] Materials: Gelatin from different animal sources, from chicken, duck, goose, cattle, pig, sheep, respectively, extracted from chicken skin, duck skin, goose skin, cowhide, pig skin, sheepskin.

[0024] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0025] 2 Screening and determination of characteristic collagen peptides

[0026] Collagen products from chickens, ducks, geese and other animals are mainly type I collagen, including COL1A1 and COL1A2 proteins, and have a certain degree of conservation. We can screen a peptide from COL1A2 protein as a characteristic collagen peptide of poultry origin. In order to screen out a stable characteristic collagen peptide for detection from the gene, the following conditions should be met: (a) the three bases adjacent to the mRNA sequence corresponding to the peptide should be argin...

Embodiment 2

[0039] 1 Materials and Reagents

[0040] Materials: Mixed gelatin samples (including: porcine gelatin with 5% chicken gelatin, porcine gelatin with 10% chicken gelatin, porcine gelatin with 20% chicken gelatin, porcine gelatin with 40% chicken gelatin, porcine gelatin with 80% chicken gelatin, pure chicken gelatin) is made from an exact mix of the gelatin from Example 1.

[0041] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0042] 2 detection method

[0043] (1) Take 0.05g gelatin sample in a 25ml measuring bottle, add a small amount of 1% NH 4 HCO 3 Soak the solution for 10 minutes, heat it at 60°C to dissolve it, and use 1% NH 4 HCO 3 Dilute the solution to the mark, shake well, filter with a microporous membrane, accurately measure 0.2ml of the subsequent filtrate, add 20μl of 2mg / ml trypsin solution, and enzymolyze at a constant temperature of 37°C f...

Embodiment 3

[0047] 1 Materials and Reagents

[0048] Materials: chicken gelatin, QQ sugar with bovine gelatin (made by adding), QQ sugar with chicken gelatin (made by adding);

[0049] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0050] 2 detection method

[0051] (1) Take 0.05-0.25g of chicken gelatin, QQ sugar added with cow gelatin, and QQ sugar added with chicken gelatin, put them in 25ml measuring bottles, add a small amount of 1% NH 4 HCO 3 Soak the solution for 10 minutes, heat it at 60°C to dissolve it, and use 1% NH 4 HCO 3 Dilute the solution to the mark, shake well, filter with a microporous membrane, accurately measure 0.2ml of the subsequent filtrate, add 20μl of 2mg / ml trypsin solution, and enzymolyze at a constant temperature of 37°C for 12h.

[0052] (2) Take 5 μl of the enzymatic hydrolysis solution and put it into the liquid mass spectrometer for d...

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Abstract

The invention discloses poultry-derived characteristic collagen peptide and an application thereof in detection of collagen hydrolysate and products thereof, and belongs to the field of detection. Theamino acid sequence of the characteristic collagen peptide is Gly-Leu-Val-Gly-Gly-Gly-Hyp-Gly-Pro-Ala-Gly-Ala-Lys, and the amino acid sequence of the characteristic collagen peptide is as shown in the specification: Gly-Gly-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Ala-Arg, Val-Gly-Pro-Ile-Gly-Pro-Ala-Gly-Asn-Arg, and Val-Gly-Gly-Gly-Gly-Asn-Arg. A poultry-derived component is containedor not is determined by detecting one or more characteristic collagen peptides. Pancreatic enzyme digestion is carried out on protein components in a sample, so the characteristic collagen peptide isdissociated and is detected by a liquid chromatograph-mass spectrometer. The method has the advantages of strong characteristics and high sensitivity, and can be used for determining the poultry-derived components in the collagen hydrolysate and products thereof.

Description

technical field [0001] The invention relates to three characteristic collagen peptides derived from poultry and its application in the detection of collagen hydrolyzate and its products, belonging to the field of medicine and food detection. Background technique [0002] Collagen is very abundant in animals, and mainly exists in connective tissues such as bones, skin, tendons and tendons of animals. Gelatin is a degradation product of collagen, which is obtained by moderate hydrolysis of animal bones, skins, tendons and tendons. It has excellent physical and chemical properties and is widely used in many fields such as food and medicine. Gelatin is mostly made of skin or bone of pigs, cattle and sheep. However, due to possible safety risks caused by diseases of poultry and livestock, and differences in halal and religious beliefs, it is of great significance to trace the source of animal-derived components of gelatin. [0003] The main component of gelatin is collagen polyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/72G01N30/86G01N30/88G01N30/89G01N30/14C07K14/78C12P21/06G01N33/68
CPCG01N30/06G01N30/72G01N30/8634G01N30/88G01N30/89G01N30/14C07K14/78C12P21/06G01N33/68G01N2030/067G01N2030/8831G01N2030/8818G01N2030/146G01N2333/78
Inventor 黄雅钦郭尚伟邓贵雅刘秀芳蒋伟
Owner BEIJING UNIV OF CHEM TECH
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