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Tissue sample extracting solution treatment method for improving color development of colloidal gold detection card

A technology for tissue samples and processing methods, applied in material inspection products, measuring devices, biological tests, etc., can solve the problems of complex organic extraction methods, inability to effectively improve color development, and low safety, and achieve simple operation and less time-consuming. , the effect of improving the accuracy

Active Publication Date: 2020-10-13
BEIJING PRIMEBIOTEK COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is an organic solvent extraction method to remove some interfering substances from the tissue extract, but it cannot effectively improve the color development, and the organic extraction method is complicated and less safe.

Method used

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  • Tissue sample extracting solution treatment method for improving color development of colloidal gold detection card
  • Tissue sample extracting solution treatment method for improving color development of colloidal gold detection card
  • Tissue sample extracting solution treatment method for improving color development of colloidal gold detection card

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preparation example Construction

[0023] The preparation methods of the tissue sample extracts involved below are the boiling method and / or the grilling method, and the specific processes of the boiling method and the grilling method are as follows:

[0024] Take lean pork samples stored in a refrigerator at -20°C as an example.

[0025] Water boiling method: Take the sliced ​​lean pork sample, put it into a centrifuge tube (it is advisable to fill the tube with three quarters full), cover the tube cover, and heat it in a water bath at a temperature above 90°C until the sample is fully cooked , take out the centrifuge tube and cool to room temperature, the liquid in the obtained tube is the tissue sample extract.

[0026] Grilling method: Cut the lean pork sample into square pieces with a thickness of 0.5cm and a length of 3cm, wrap them in aluminum foil, and sandwich them between the splints of the extractor. Keep the temperature of the extractor at 140°C for about 30-40s Finally, liquid flows out from the a...

Embodiment 1

[0034] Embodiment 1: get the pig lean meat sample extraction solution 1.35ml that adopts poaching method to obtain in the 10ml centrifuge tube, add the hydrogen peroxide 0.15ml that mass fraction is 30% (hydrogen peroxide is added in the tissue sample extraction solution after Mass fraction is 3%), after mixing, let stand for 10min.

Embodiment 2

[0035] Embodiment 2: get the pig lean meat sample extracting solution 1.35ml that adopts pinch roasting method to obtain in the 10ml centrifuge tube, add the hydrogen peroxide 0.15ml that mass fraction is 30% (hydrogen peroxide is added in the tissue sample extracting solution after Mass fraction is 3%), after mixing, let stand for 10min.

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Abstract

The invention provides a tissue sample extracting solution treatment method for improving color development of a colloidal gold detection card. According to the treatment method, a tissue sample extracting solution is subjected to oxidation treatment, an adopted oxidizing agent is hydrogen peroxide or urea peroxide, and a treatment mode is that a hydrogen peroxide solution is added into the tissuesample extracting solution or a urea peroxide solution is added into the tissue sample extracting solution or urea peroxide is added onto a sample pad of the colloidal gold detection card. Accordingto the tissue sample extracting solution treatment method for improving the color development of the colloidal gold detection card, the tissue sample extracting solution is oxidized so that an influence of a part of interfering substances is eliminated, the color development of the detection card is improved, and detection accuracy is increased.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and in particular relates to a method for processing tissue sample extract liquid for improving the color development of a colloidal gold detection card. Background technique [0002] During the R&D and production experiments, the following conditions were found during the test of the CLE colloidal gold test card: the gray value of the test card for some tissue sample extracts was low, resulting in inaccurate test results. For this part of the samples, due to the interfering substances in the tissue sample extract, the color development of the T and C lines of the test card is relatively light. At present, there is a method of extracting tissue extracts with organic solvents to remove some interfering substances, but it cannot effectively improve color development, and the organic extraction methods are complicated and less safe. Therefore, there is an urgent need to develop a simp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/558G01N33/58
CPCG01N33/531G01N33/558G01N33/587
Inventor 许春苹邓乾民孙清刘冰冰
Owner BEIJING PRIMEBIOTEK COMPANY