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Passive micro-mixer for chemiluminescence detection and use method of passive micro-mixer

A chemiluminescence detection and micro-mixer technology is applied in the fields of microfluidic technology and biochemical detection, which can solve the problems such as the inability to achieve the strongest luminescence intensity, the complex structure of the microfluidic chip, and the increase of the length of the detection channel, so as to speed up the chemical reaction. speed, shorten the mixing distance, and improve the effect of detection sensitivity

Active Publication Date: 2020-10-16
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The reported microfluidic chips related to chemiluminescence detection have the following disadvantages: 1. The channel structure of the microfluidic chip is linear, and the sample is not mixed enough to achieve the strongest luminous intensity. For example, the patent application number is CN201610517808.6 A microfluidic chip for chemiluminescence detection and its detection method disclosed in the Chinese patent; 2. The structure of the microfluidic chip is complex, which increases the difficulty of manufacturing. chemiluminescence microfluidic chip; 3. the detection channel is helical, and the sample injection mixing distance is long, which increases the length of the detection channel. Fluidic chip

Method used

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  • Passive micro-mixer for chemiluminescence detection and use method of passive micro-mixer
  • Passive micro-mixer for chemiluminescence detection and use method of passive micro-mixer
  • Passive micro-mixer for chemiluminescence detection and use method of passive micro-mixer

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A cross-type passive micromixer for chemiluminescent detection, such as figure 1 As shown, it includes a micro-mixer matrix 1 and a cross-shaped channel arranged inside it. The four directions of the cross-shaped channel are: the sample channel 4 to be tested, the first sheath flow channel 6-1, the detection channel 10 and the second channel. The outflow end of the sheath flow channel 6-2, the sample channel 4 to be tested, the first sheath flow channel 6-1, and the second sheath flow channel 6-2 are connected to the inflow end of the detection channel 10 through the mixing chamber 2, and the mixing chamber 2 is connected to the detection channel 10 through a narrow connection channel 9, the outflow end of the detection channel 10 is connected to the waste liquid pool 8 through the waste liquid outflow channel 7, and the inflow end of the sample channel 4 to be tested is connected to the sample liquid storage pool to be tested 3, the inflow ends of the first sheath flow...

Embodiment 2

[0047] A Y-type passive micro-mixer for chemiluminescence detection, comprising a micro-mixer base 1 and a Y-shaped channel arranged inside it, such as Figure 5 As shown, the three directions of the Y-shaped channel are: the sample channel 4 to be tested, the sheath flow channel 6, the detection channel 10, the sample channel 4 to be tested, the outflow end of the sheath flow channel 6 and the inflow end of the detection channel 10 Connected through the mixing chamber 2, the mixing chamber 2 and the detection channel 10 are connected through a narrow connection channel 9, the outflow end of the detection channel 10 is connected to the waste liquid pool 8 through the waste liquid outflow channel 7, and the inflow of the sample channel 4 to be tested The end is connected with the sample storage tank 3 to be tested, and the inflow end of the sheath flow channel 6 is connected with the sheath flow storage tank 5 .

[0048] The detection channel 10 is composed of more than three m...

Embodiment 3

[0053] A method for using the cross-type passive micro-mixer of embodiment 1, adopting the cross-type passive micro-mixer of embodiment 1 to carry out chemiluminescence detection transition metal cobalt ion concentration, comprising the steps:

[0054] (1) To clean the flow path, use 1.0mol L sequentially -1 Wash with NaOH for 30 minutes, then wash with ultrapure water for 5 minutes;

[0055] (2) The sample storage tank 3 to be tested, the first sheath flow storage tank 5-1, and the second sheath flow storage tank 5-2 are respectively used to hold the cobalt chloride solution to be tested, the hydrogen peroxide solution and the Minol solution;

[0056] Negative pressure is applied to the waste liquid tank 8, or positive pressure is applied to the sample storage tank 3 to be tested, the first sheath flow storage tank 5-1, and the second sheath flow storage tank 5-2 to drive the cobalt chloride solution , hydrogen peroxide solution and luminol solution move to the detection ch...

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Abstract

The invention discloses a passive micro-mixer for chemiluminescence detection. The micro-mixer comprises a micro-mixer base body, and a to-be-detected sample channel, a sheath flow channel and a detection channel which are arranged in the micro-mixer base body, the outflow ends of the to-be-detected sample channel and the sheath flow channel are connected with the inflow end of the detection channel through a mixing chamber, the detection channel is formed by connecting a plurality of mixing units in series, a Z-shaped microstructure baffle is arranged in each mixing unit, a gap is reserved between each Z-shaped microstructure baffle and the side wall of the channel in the corresponding mixing unit, and the Z-shaped microstructure baffles in every two adjacent mixing units are arranged ina mirror symmetry mode. The passive micro-mixer can effectively improve the mixing quality, shorten the mixing distance and enhance the chemiluminiscence intensity, so that the chemiluminiscence detection sensitivity is improved, and the passive micro-mixer is simple in structure and has a wide application prospect. The invention further discloses a use method of the passive micro-mixer, the operation is simple, and chemiluminiscence detection can be rapidly and accurately carried out.

Description

technical field [0001] The invention belongs to the technical fields of microfluidic technology and biochemical detection, and in particular relates to a passive micro-mixer for chemiluminescent detection and a method for using the same. Background technique [0002] Microfluidics is a new technology for manipulating particles and fluids at the micron or submicron scale. As an important carrier of microfluidic technology, microfluidic chips can realize a series of operations such as sample injection, pretreatment, mixing, reaction, and detection through structures such as microchannels, microvalves, and micropumps, which greatly shortens sample processing time and facilitates detection. The resolution is significantly improved. At present, microfluidic chips are widely used in the fields of disease diagnosis, environmental monitoring, and food science due to their advantages of integration, miniaturization, and low consumption, and have great development potential and appli...

Claims

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Application Information

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IPC IPC(8): B01F13/00G01N1/38G01N21/76
CPCG01N21/76G01N1/38B01F33/3017
Inventor 蒋炳炎袁帅周明勇
Owner CENT SOUTH UNIV
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