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Technical method for producing aspergillus niger by using white spirit distiller's grains

A technology for white distiller's grains and Aspergillus niger, which is applied in the field of using white distiller's grains to produce Aspergillus niger, can solve the problems of difficulty in separating bacterial species and low number of viable bacteria, and achieves the effects of high number of viable bacteria, low fermentation cost, and efficient extraction and utilization.

Pending Publication Date: 2020-10-16
四川润格生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there have been studies on the use of distiller's grains to produce bacteria, but it inevitably has the problems of difficult separation of strains and low number of viable bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of distiller's grain medium:

[0040] (1) Mix and stir distiller's grains and water according to the mass ratio of 1:4, let stand for 3 minutes, remove impurities floating on the water surface, filter the stirred mixture, collect the filtrate, and dry at 38°C until the water content is 10% , to collect dry matter;

[0041] (2) In parts by weight, put 30 parts of dry matter, 10 parts of glucose and 3 parts of beef extract powder into a steam explosion device to carry out steam explosion under the condition of a pressure of 1.2 MPa, maintain the pressure for 20 minutes, collect and explode thing;

[0042] (3) In parts by weight, 60 parts of explosives, 20 parts of corn dregs, 22 parts of water and 12 parts of rice bran were put into a mixer and stirred evenly, and the stirred mixture was collected and put into a fermentation tank, and naturally ventilated for 5 days;

[0043] (5) After the ventilation finishes, add yeast powder in the fermentation tank, seal...

Embodiment 2

[0053] Preparation of distiller's grain medium:

[0054] (1) Mix and stir distiller's grains and water according to the mass ratio of 1:5, let stand for 5 minutes, remove impurities floating on the water surface, filter the stirred mixture, collect the filtrate, and dry at 42°C until the water content is 15% , to collect dry matter;

[0055] (2) In parts by weight, put 35 parts of dry matter, 15 parts of glucose and 6 parts of beef extract powder into a steam explosion device and carry out steam explosion under the condition of a pressure of 1.5MPa. thing;

[0056] (3) In parts by weight, 65 parts of explosives, 25 parts of corn dregs, 24 parts of water and 15 parts of rice bran were put into a mixer and stirred evenly, and the stirred mixture was collected and put into a fermentation tank, and naturally ventilated for 7 days;

[0057] (5) After the ventilation finishes, add yeast powder in the fermentation tank, seal the fermentation tank for 2 days, stir with 200r / min for ...

Embodiment 3

[0067] Preparation of distiller's grain medium:

[0068] (1) Mix and stir distiller's grains and water according to the mass ratio of 1:4.5, let it stand for 4 minutes, remove impurities floating on the water surface, filter the stirred mixture, collect the filtrate, and dry at 40°C until the water content is 12% , to collect dry matter;

[0069] (2) In parts by weight, put 33 parts of dry matter, 12 parts of glucose and 4 parts of beef extract powder into a steam explosion device to carry out steam explosion under the pressure of 1.3MPa, maintain the pressure for 18min, collect and explode thing;

[0070] (3) In parts by weight, 63 parts of explosives, 23 parts of corn dregs, 22 parts of water and 13 parts of rice bran were put into a mixer and stirred evenly, and the stirred mixture was collected and put into a fermentation tank, and naturally ventilated for 6 days;

[0071] (5) After the ventilation finishes, add yeast powder in the fermentation tank, seal the fermentatio...

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Abstract

The invention discloses a technical method for producing aspergillus niger by using white spirit distiller's grains, and belongs to the technical field of fermentation engineering. The technical method comprises the following steps: preparing a culture medium from the remaining distiller's grains after fermentation of white spirit, carrying out activating of an aspergillus niger strain, inoculating the activated aspergillus niger strain into the culture medium of the white spirit distiller's grains to induce spore production, and carrying out fractional precipitation with ammonium sulfate to obtain the aspergillus niger. According to the technical method for producing the aspergillus niger by utilizing the white spirit distiller's grains, the technical problems of difficulty in strain separation and low viable count in the process of producing the aspergillus niger by utilizing the white spirit distiller's grains in the prior art can be solved, the yield of the aspergillus niger is remarkably increased, and the problem that nutrient substances in the white spirit distiller's grains cannot be fully utilized is solved.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a technical method for producing Aspergillus niger by using distiller's grains. Background technique [0002] Aspergillus niger is a common species in the Aspergillus genus, which is widely distributed in grain, vegetable products and soil. Aspergillus niger can be used as a fermentation strain in the food industry, such as vinegar production, bran koji production, citric acid fermentation, etc. It is mainly used to produce amylase, glucoamylase and citric acid secreted by Aspergillus niger , gluconic acid, gallic acid, etc.; in the bio-fertilizer industry, Aspergillus niger has the effect of cracking macromolecular organic matter and insoluble inorganic matter, which is convenient for crop absorption and utilization, improves soil structure, enhances soil fertility, and increases crop yield; it can also be used To determine manganese, copper, molybdenum, zinc a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/685
CPCC12N1/14
Inventor 彭楠常章兵田建平梁运祥
Owner 四川润格生物科技有限公司
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