Technical method for producing aspergillus niger by using white spirit distiller's grains
A technology for white distiller's grains and Aspergillus niger, which is applied in the field of using white distiller's grains to produce Aspergillus niger, can solve the problems of difficulty in separating bacterial species and low number of viable bacteria, and achieves the effects of high number of viable bacteria, low fermentation cost, and efficient extraction and utilization.
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Embodiment 1
[0039] Preparation of distiller's grain medium:
[0040] (1) Mix and stir distiller's grains and water according to the mass ratio of 1:4, let stand for 3 minutes, remove impurities floating on the water surface, filter the stirred mixture, collect the filtrate, and dry at 38°C until the water content is 10% , to collect dry matter;
[0041] (2) In parts by weight, put 30 parts of dry matter, 10 parts of glucose and 3 parts of beef extract powder into a steam explosion device to carry out steam explosion under the condition of a pressure of 1.2 MPa, maintain the pressure for 20 minutes, collect and explode thing;
[0042] (3) In parts by weight, 60 parts of explosives, 20 parts of corn dregs, 22 parts of water and 12 parts of rice bran were put into a mixer and stirred evenly, and the stirred mixture was collected and put into a fermentation tank, and naturally ventilated for 5 days;
[0043] (5) After the ventilation finishes, add yeast powder in the fermentation tank, seal...
Embodiment 2
[0053] Preparation of distiller's grain medium:
[0054] (1) Mix and stir distiller's grains and water according to the mass ratio of 1:5, let stand for 5 minutes, remove impurities floating on the water surface, filter the stirred mixture, collect the filtrate, and dry at 42°C until the water content is 15% , to collect dry matter;
[0055] (2) In parts by weight, put 35 parts of dry matter, 15 parts of glucose and 6 parts of beef extract powder into a steam explosion device and carry out steam explosion under the condition of a pressure of 1.5MPa. thing;
[0056] (3) In parts by weight, 65 parts of explosives, 25 parts of corn dregs, 24 parts of water and 15 parts of rice bran were put into a mixer and stirred evenly, and the stirred mixture was collected and put into a fermentation tank, and naturally ventilated for 7 days;
[0057] (5) After the ventilation finishes, add yeast powder in the fermentation tank, seal the fermentation tank for 2 days, stir with 200r / min for ...
Embodiment 3
[0067] Preparation of distiller's grain medium:
[0068] (1) Mix and stir distiller's grains and water according to the mass ratio of 1:4.5, let it stand for 4 minutes, remove impurities floating on the water surface, filter the stirred mixture, collect the filtrate, and dry at 40°C until the water content is 12% , to collect dry matter;
[0069] (2) In parts by weight, put 33 parts of dry matter, 12 parts of glucose and 4 parts of beef extract powder into a steam explosion device to carry out steam explosion under the pressure of 1.3MPa, maintain the pressure for 18min, collect and explode thing;
[0070] (3) In parts by weight, 63 parts of explosives, 23 parts of corn dregs, 22 parts of water and 13 parts of rice bran were put into a mixer and stirred evenly, and the stirred mixture was collected and put into a fermentation tank, and naturally ventilated for 6 days;
[0071] (5) After the ventilation finishes, add yeast powder in the fermentation tank, seal the fermentatio...
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