Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Simple method for culturing probiotics suitable for aquaculture

A culture method and aquaculture technology, applied in the field of microbial fermentation, can solve the problems of high cost of use, high price, easy inactivation of live bacteria preparations, etc., and achieve the effect of ensuring production quality

Inactive Publication Date: 2020-10-16
LIAONING OCEAN & FISHERIES SCI RES INST
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the probiotics industry in the aquatic industry, such as the lack of counterpart strains developed for aquaculture characteristics, live bacterial preparations are easily inactivated during storage and transportation, the market is not standardized, and the quality of aquatic probiotics is uneven. Aquaculture practitioners have insufficient ability to identify the quality of probiotics. The main contradiction is: the contradiction between farmers’ demand for high-quality probiotic products and the low cost performance of aquatic probiotic products
At present, probiotic products for aquaculture on the market are of good quality, but the price is high, and the cost of using large water surfaces for farmers is relatively high; while products with low prices have fewer live bacteria and more miscellaneous bacteria, and the product quality cannot be guaranteed
Probiotics products go from the manufacturer to the front line of breeding. In addition to the production cost of the manufacturer, there are also costs such as packaging, logistics, and intermediary price differences. And the number of viable bacteria decreased during storage
In recent years, some farmers have used the existing conditions to purchase strains and culture media to carry out fermentation operations themselves. However, due to irregular operation procedures, the fermented bacterial liquid contains fewer bacteria and more bacteria, which affects the use effect. Therefore, There is an urgent need for a simple probiotic culture method suitable for the front line of aquaculture. Aquaculture enterprises and farmers do not need to use fermenters. This method can obtain high-quality bacterial liquid that meets the needs of aquaculture, ensuring the effect of probiotics and reducing the cost of aquaculture. cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Simple method for culturing probiotics suitable for aquaculture
  • Simple method for culturing probiotics suitable for aquaculture
  • Simple method for culturing probiotics suitable for aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Step 1: The temperature in the lactic acid bacteria cultivation room is required to be controllable, and an ultraviolet lamp is installed according to the room space, with an average of 2w per cubic meter;

[0110] Step 2: Use an open plastic barrel as a lactic acid bacteria culture container, cut a transparent plastic cloth according to the size of the mouth of the barrel for sealing, cover the mouth of the barrel with the plastic cloth, and tie it tightly around the mouth. The center of the plastic cloth is perforated to facilitate the passage of the inflatable tube, the contact between the plastic cloth and the trachea is tied tightly with a rope, and all items are sterilized by ultraviolet light for 1 hour before inoculation;

[0111] Step 3: Put the beaker, measuring cylinder and other tools used in the expansion process into water and boil for 10 minutes for disinfection;

[0112] Step 4: The inflatable pipeline is filtered through a precision filtration system; ...

Embodiment 2

[0125] A simple method for cultivating probiotics suitable for aquaculture, the operation of the method is as follows:

[0126] Step 1: The temperature in the subtilis spore culture room is required to be controllable, and an ultraviolet lamp is installed according to the room space, with an average of 3w per cubic meter;

[0127] Step 2: Use an open plastic barrel as a subtilis spore culture container, cut a transparent plastic cloth according to the size of the mouth of the barrel for sealing, cover the mouth of the barrel with the plastic cloth, and tie the surroundings tightly with ropes. The center of the plastic cloth is perforated to facilitate the passage of the inflatable tube, the contact between the plastic cloth and the trachea is tied tightly with a rope, and all items are sterilized by ultraviolet light for 2 hours before inoculation;

[0128] Step 3: Put the beaker, measuring cylinder and other tools used in the expansion process into water and boil for 15 minut...

Embodiment 3

[0142] A simple method for cultivating probiotics suitable for aquaculture, the operation of the method is as follows:

[0143] Step 1: The temperature in the lactic acid bacteria cultivation room is required to be controllable, and an ultraviolet lamp is installed according to the room space, with an average of 5w per cubic meter;

[0144] Step 2: Use an open plastic barrel as a probiotic culture container, cut a transparent plastic cloth according to the size of the mouth of the barrel for sealing, cover the mouth of the barrel with the plastic cloth, and tie it tightly around the mouth. The center of the plastic cloth is perforated to facilitate the passage of the inflatable tube, and the contact between the plastic cloth and the trachea is tied tightly with a rope. Before inoculation, all items are sterilized by ultraviolet light for 3 hours;

[0145] Step 3: Put the beaker, measuring cylinder and other tools used in the expansion process into water and boil for 30 minutes...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
solid contentaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for applying a bacillus bacterial liquid to the breeding of sea cucumbers. The method is operated as follows: sea cucumber seedlings with a single weight of 1-10 g arecultured at a density of 100-500 g / m<3>. The bacillus bacterial liquid cultivated by a simple culture method is mixed in feed according to a concentration ratio of 10<7> or 10<9> cfu / g by a turbidimetric method for feeding sea cucumbers, and a daily feeding amount is 3-10% of the sea cucumber body weight. The single weight of the sea cucumber seedlings is 1-10 g, and the breeding density is 100-500 g / m<3>. The bacillus bacterial liquid cultivated by the method of the invention is mixed in the feed at the concentration ratio of 10<7> or 10<9> cfu / g by the turbidimetric method for feeding the sea cucumbers. The daily feeding amount of the feed is 3-10% of the sea cucumber body weight.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a simple culture method for probiotics suitable for aquaculture. Background technique [0002] Probiotics are currently playing a huge role in aquaculture. Probiotics themselves are rich in nutrients, which can directly provide available amino acids and vitamins to aquatic animals. Moreover, probiotics can produce a variety of organic acids, which can promote the absorption of calcium, iron ions and vitamin D in water-cold animals. Probiotics can maintain the balance of flora in the stomach and intestine of aquatic animals, improve the function of the gastrointestinal tract, improve the quality of feed, and improve the utilization rate of digestion. They can also synthesize a variety of amino acids and vitamins required by the host animal to maintain life activities in the intestine. etc. for use by host animals. Probiotics can produce various enzymes during the metabolic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80B01D39/16B01D39/18B01D46/54B01D46/00C12R1/07C12R1/20C12R1/225C12R1/44C12R1/01
CPCC12N1/20A23K10/18A23K50/80B01D39/16B01D39/18B01D46/54B01D46/00B01D2239/10
Inventor 李石磊董颖王旭达周遵春王笑月刘忠颖叶博陈仲关晓燕
Owner LIAONING OCEAN & FISHERIES SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com