In-vivo rabbit small intestine movement test box and test device

Abstract

The invention discloses an in-vivo rabbit small intestine movement test box and a test device. A box body of the test box is of a closed square hollow structure with an upper opening; constant-temperature heating elements are fixedly arranged outside at least one transverse side wall and/or the bottom wall of the box body; the upper end of one longitudinal side wall of the box body is connected with an inclined groove inclining outwards and upwards; upward groove walls are arranged on the two sides of the inclined groove, and constant-temperature heating elements are fixedly arranged outside the bottom wall of the inclined groove. The two transverse side walls or the side walls and the bottom wall which are longitudinally connected with the inclined groove of the box body are magnetic metal walls, and the bottoms of magnetic small intestine fixers are magnetically pasted on the magnetic metal walls. The test device comprises the test box and is fixedly arranged on the lower portion ofa support; a tension transducer is fixedly arranged on the support above the test box; the upper end of a tension line is connected with the tension transducer, and the lower end of the tension line is perpendicular to the interior of the test box. During in-vivo tests, oxygen does not need to be independently supplied to rabbits, interference of respiration and heartbeat on small intestine movement can be effectively eliminated, the intestinal loop is long in survival time, the rabbits can be repeatedly tested, and the test box and the test device can be suitable for teaching and drug research application.

Description

technical field [0001] The invention belongs to the technical field of medical test equipment, and specifically relates to an in vivo rabbit small intestine movement test box with simple structure, low requirements for test conditions, which can effectively eliminate the interference of breathing and heartbeat on small intestine movement, and has a long survival time of rabbit intestinal loops. test device. Background technique [0002] At present, in medical teaching and drug research, the small intestine motility test of isolated rabbits is generally used, and there are only a few reports on the in vivo test methods and devices. The in vitro test technology is relatively mature, but the conditions are relatively high, such as the need for oxygen and special nutrient solution. Due to certain risks and difficulties in the acquisition, use, storage, and transportation of oxygen, some units directly use air instead. Without oxygen, it will cause insufficient oxygen supply in ...

AI Technical Summary

Problems solved by technology

The in vitro test technology is relatively mature, but the conditions are relatively high, such as the need for oxygen and special nutrient solution. Due to certain risks and difficulties in the acquisition, use, storage, and transportation of oxygen, some units directly use air instead. Without oxygen, it will cause insufficient oxygen supply in the isolated small intestine, tissue cell damage, weakened movement activity, and shortened survival time; secondly, because the in vitro test can only survive for about half an hour even at a constant temperature of 38°C without oxygen, for Drug research that requires long-term observation is difficult to meet the requirements

In addition, because constant temperature technology and anti-interference technology (interference of breathing, heartbeat, etc.) are difficult to solve, some in vivo test units do not have constant temperature equipment, so they use the method of visual observation to determine the movement of the small intestine, resulting in test results that are not objective and accurate.

In addition, part of the in vivo test is to pull out the small intestine of the rabbit and fix it, then directly drop the reagent on the surface of the intestine, and use the blood circulation of the rabbit itself to maintain the body temperature and nutrient supply of the small intestine; but because the reagent is easy to lose, not only The number of small intestine motility waveforms that can be effectively observed is limited, and prolonged dry exposure will also affect the normal movement of the small intestine; and the reason for the temperature difference between the body and the outside is that it is difficult to maintain the body temperature of the small intestine only by blood circulation in rabbits, which will affect the physiological function of the small intestine. The function has a certain impact, which affects the accuracy of the test results; because the exposed small intestine of the rabbit is affected by long-term drying and temperature difference, it is easy to cause the survival time of the intestinal loop is not long, and the rabbit can only be carried out once or a limited number of times. Experiments lead to higher experiment costs and are not suitable for teaching applications

In order to solve the above-mentioned problems in the in vivo test, there are also water-isolated constant temperature heating, and then a test box with an inner tank for containing the small intestine and reagents is used to carry out the in vivo test, but it is also inconvenient to operate in the inner tank. The fixed length, height and position in the tank are difficult to adjust, which makes it difficult to effectively prevent the interference of breathing and heartbeat on the test; in addition, because the small intestine of the rabbit in the prior art is fixed through the frog nail holes at the bottom of the inner tank by frog nails , it is difficult to reliably ensure the appropriate degree of tightness of small intestine fixation, and it is easy to cause too tight to affect blood circulation, resulting in distortion of observation data and short survival time of intestinal loops, while too loose fixation is unstable, and it is easy to fall out during the test and lead to test failure

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