Hyperoxia ventilating pore plate device for organoid sphere culture

Abstract

The invention discloses a hyperoxia ventilating pore plate device for organoid sphere culture. The device comprises a pore cover plate (1), a culture pore unit (2) and a pore plate base (3), wherein the pore plate base (3) is provided with a cavity (30) with a through bottom hole (31), and the culture pore unit (2) is embedded in the cavity (30). By arranging the through bottom hole as a ventilating window, the cell culture process can be ensured to have a sufficient dissolved oxygen environment, the problem of insufficient dissolved oxygen at the bottom of the near-pore plate of the organoidcell sphere is solved, the rapid growth of the organoid cell sphere is effectively promoted, the cell activity is improved, the cell culture efficiency is increased, and disintegration and apoptosis of the organoid cell sphere caused by long-term culture are avoided; and a new research tool is expected to be provided for constructing a three-dimensional organoid cell sphere model with relatively high oxygen demand. Compared with existing culture devices in the market, the device has the advantages that the gas mass transfer efficiency is higher, the boundary of cultured organoid cell spheres is clear, the cell aggregation compactness is high, and the disintegration phenomenon is avoided.

Description

technical field [0001] The invention relates to the technical field of biological cell culture, in particular to the field of organoid culture, and in particular to a high-oxygen permeable orifice plate device for organoid spheroid culture. Background technique [0002] In vitro cell culture is a commonly used and important technical method in cell biology research methods, and is also an important technical method for tissue engineering and drug screening. In the process of cell culture, the microenvironment of cell culture plays a key role in cell growth, such as gas environment, especially the content of oxygen concentration is very necessary in the process of cell physiological metabolism. Oxygen participates in the tricarboxylic acid cycle of cells, providing energy for cell survival, metabolism and synthesis. Some cells can obtain energy through glycolysis in hypoxic environment in vitro, but most normal somatic cells cannot be cultivated and applied on a large scale ...

AI Technical Summary

Problems solved by technology

For organoid cell spheroids with a large oxygen demand, hypoxia will lead to slow cell growth, and severe cell damage will occur in a large area, which will bring serious losses to experimental research.

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