High-yield fermentation method of carotenoid high-yield sporobolomyces strain and application of method
A technology of carotene and yeast strains, applied in the field of microbial fermentation and food processing
Pending Publication Date: 2020-10-20
YANCHENG INST OF TECH +1
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Problems solved by technology
[0006] The technical problem solved: in view of the above technical problem, the present invention provides a high-yield fermentation method and its application of carotenoid high-yielding yeast strains, and solves how to properly optimize nutrient factors, growth-promoting factors and various fermentation conditions, as far as possible Technical Problems of Improving the Production of Carotenoids Produced by High-yielding Mutant Strain of Saccharomyces sporogenes
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preparation example Construction
[0037] 3.1 Preparation of seed solution
[0038] Continuously pick 3-4 rings of spore-forming yeast activated on solid seed medium for 3 days into a 5mL plastic centrifuge tube filled with 2mL sterile water. After mixing evenly, pipette 0.2mL respectively into a bottle containing 50mL liquid In a 250mL Erlenmeyer flask of the seed medium, cultivate at a speed of about 180r / min at 16°C for 36-48h, and add sterile water to prepare the seed solution. Measure and control the bacterial content of the seed liquid by microscope counting method to 3.0×10 8 ~4.0×10 8 per mL, and stored in a refrigerator at 4°C until use.
[0039] 3.2 Ultraviolet mutagenesis of sporogenous yeast strains
[0040] Take 5mL of the prepared seed solution, add it to a sterile petri dish with a diameter of 6cm, place the sterile petri dish on a magnetic stirrer at a distance of 30cm from a 30w, 254nm ultraviolet lamp, and irradiate it for 30-60s, so that no ultraviolet light The irradiated seed solution w...
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The invention discloses a high-yield fermentation method of a carotenoid high-yield sporobolomyces strain and application of the method, and belongs to the technical field of microbial fermentation and food processing. The preservation number of the bacterial strain is CGMCC No.17429, and the preservation name of the bacterial strain is Sporobolomyces reseu GCJM3-6. Optimization is carried out through a single factor test and an orthogonal test, and suitable fermentation conditions of the strain are determined as follows: the inoculum size is 5% (V / V), oscillation speed is 180 r / min, the bacterium content of a high-yield sporotrichum strain seed solution is 6.0x10<8> to 8.0x10<8> pieces / mL, the liquid filling ratio is 5%-10% (V / V), initial fermentation pH is 6.0, fermentation temperature is 16-18 DEG C, fermentation time is 60-78 hours, the liquid fermentation culture medium is prepared from 20-22 g / L of cane sugar, 12-14 g / L of peptone, 10-12 g / L of yeast extract, 0.4-0.6 mg / L of lycopene and 0.2-0.6 mg / L of riboflavin. The optimized fermentation condition significantly improves the yield of carotenoid, the average yield is up to 822.6-898.4 [mu]g / g (dry thallus), and meanwhile, the fermentation condition of the high-yield sporotrichum has the advantages of simple culture medium, mild reaction, high yield, low production cost, environmental friendliness and the like.
Description
technical field [0001] The invention belongs to the technical field of microbial fermentation and food processing, and in particular relates to a high-yield fermentation method of a carotenoid high-yielding sporozoan yeast strain and an application thereof. Background technique [0002] Carotenoids, represented by β-carotene, lycopene, torumin, and anthocyanins, are a class of yellow, orange-red, or red polyene compounds that have the biological activity of being converted into vitamin A. It has various physiological functions such as preventing cancer and cardiovascular diseases. At present, carotenoids have been identified as A-type nutritional pigments by international organizations such as FAO and WHO, and have been approved as food additives with dual functions of nutrition and coloring in more than 50 countries and regions, and are widely used in health food and pharmaceutical and cosmetic industries. . The extraction of carotenoids from natural fruits and vegetables...
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IPC IPC(8): C12N1/16C12P23/00C12R1/645
CPCC12P23/00C12N1/145C12R2001/645
Inventor 李朝霞陈慧李丁杨成飞仇明梁慧星
Owner YANCHENG INST OF TECH