Growth-promoting strain screening method based on high-throughput sequencing and application of method
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A high-throughput, sequencing technology, applied in the field of soil microbial isolation, can solve the problem that the utilization potential of soil microbial resources cannot be fully utilized
Active Publication Date: 2020-10-20
SOUTH CHINA AGRI UNIV
View PDF5 Cites 5 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Stress conditions are ubiquitous in terrestrial ecosystems, and unfavorable environmental conditions can not only inhibit the population of beneficial soil microorganisms, but also affect their functions, so that the utilization potential of soil microbial resources cannot be fully utilized
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0033] Example 1 Screening method for growth-promoting fungal strains based on high-throughput sequencing
[0034] This example uses seven soil pHs as functional gradients to provide a screening method for growth-promoting fungal strains based on high-throughput sequencing. The specific experimental methods and results are as follows:
[0035] 1. Experimental method
[0036] A screening method for growth-promoting fungal strains based on high-throughput sequencing, comprising the following steps:
[0037] S1. Collect the soil 0-20cm above the ground from acidic red soil orchards in subtropical regions, pass it through a 2mm sieve after air-drying, and set aside.
[0038] S2. Adjust soil pH: take 7 parts of 2kg dry soil, add 1.0g / kg and 2.0g / kg sulfur powder to 2 parts of soil respectively, add 1.5g / kg, 3.0g / kg, 4.5g / kg to 4 parts of soil respectively , 7.5g / kg of calcium hydroxide, mix thoroughly, and the last part of soil is the original soil. 7 soils were cultivated in larg...
Embodiment 2
[0054] Example 2 Screening method for growth-promoting bacterial strains based on high-throughput sequencing
[0055] This embodiment uses seven soil pHs as functional gradients to provide a screening method for growth-promoting bacterial strains based on high-throughput sequencing. The specific experimental methods and results are as follows:
[0056] 1. Experimental method
[0057] A screening method for growth-promoting bacterial strains based on high-throughput sequencing, comprising the following steps:
[0058] S1. Collect the soil 0-20cm above the ground from acidic red soil orchards in subtropical regions, pass it through a 2mm sieve after air-drying, and set aside.
[0059] S2. Adjust soil pH: take 7 parts of 2kg dry soil, add 1.0g / kg and 2.0g / kg sulfur powder to 2 parts of soil respectively, add 1.5g / kg, 3.0g / kg, 4.5g / kg to 4 parts of soil respectively , 7.5g / kg of calcium hydroxide, mix thoroughly, and the last part of soil is the original soil. 7 soils were cult...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
diameter
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses a growth-promoting strain screening method based on high-throughput sequencing and an application of the method. According to the method, an effective soil function screening gradient is constructed so as to form a gradient change difference between the growth of a test plant and the composition of a soil microbial community, and the community composition of the microorganisms in the soil is analyzed by combining a widely-used, low-cost and high-accuracy high-throughput sequencing technology; and by carrying out regression analysis through the absolute abundance of thedominant strain OTU and the biomass of the test plant, whether the dominant strain is a potential growth-promoting strain or not is judged, the dominant potential growth-promoting strain with high absolute abundance is separated, and a tie-back test is carried out to verify the growth-promoting effect of the dominant strain. According to the method, the growth-promoting strains are screened directly depending on the growth-promoting effect, and uncertainty and false positive of the strains screened according to functional genes and a functional selection culture medium in practical applicationcan be effectively avoided; and in addition, the method is clear in target, higher in success rate and wide in application prospect in screening growth-promoting strains.
Description
technical field [0001] The invention belongs to the technical field of soil microorganism separation. More specifically, it relates to a method for screening growth-promoting strains based on high-throughput sequencing and its application. Background technique [0002] Soil microorganisms are the most active and important part of the soil due to their large number and variety, and are known as the engine of the biogeochemical cycle process of the earth's key elements. For plants, some soil microorganisms can improve their nutritional status, enhance their tolerance to adversity, reduce the occurrence of diseases and insect pests, and promote plant growth and development. For example, mycorrhizal fungi. As a widely distributed soil fungus, arbuscular mycorrhizal fungi (AMF) can establish a mutually beneficial symbiotic relationship with more than 80% of plant roots on land. Once the symbiotic relationship is established, AMF can greatly improve the host plant's uptake of v...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more