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Application of disulfiram in preparation of drugs capable of resisting head and neck cancer and fibrosis

A technology for disulfiram and head and neck cancer, applied in the field of drug development and application

Inactive Publication Date: 2020-10-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a small molecule drug, the use of disulfiram in head and neck cancer and pulmonary fibrosis has not been reported

Method used

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  • Application of disulfiram in preparation of drugs capable of resisting head and neck cancer and fibrosis
  • Application of disulfiram in preparation of drugs capable of resisting head and neck cancer and fibrosis
  • Application of disulfiram in preparation of drugs capable of resisting head and neck cancer and fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Research on Disulfiram Function Regulatory Effects on Head and Neck Cancer Cells

[0033] Disulfiram can significantly inhibit the proliferation of human head and neck cancer cells, and promote the apoptosis of human head and neck cancer cells. It provides an important basis for the clinical treatment of head and neck cancer. This example further demonstrates the specific mechanism of action of disulfiram on head and neck cancer cell lines, and also provides the possibility for disulfiram to related diseases with similar molecular mechanisms.

[0034] 1 Experimental materials

[0035] 1.1 Cell lines: HN30 cell line, Cal27 cell line, HN4 cell line, HN12 cell line, SCC9 cell line, SCC25 cell line.

[0036] 1.2 Drugs and reagents: MTT detection kit (Biyuntian), cell apoptosis detection kit (Biyuntian).

[0037] 1.3 Main equipment: BD LSRFortessa flow cytometer; multifunctional microplate reader;

[0038] 2 Methods and Results

[0039] 2.1 Experimental metho...

Embodiment 2

[0065] Example 2. Study on the regulating effect of disulfiram on pulmonary fibrosis

[0066]In this experiment: the preparation method of disulfiram used in animal experiments is as follows, dissolve disulfiram in DMSO, the concentration is 2g / ml, then mix 25 μl with 400 μl ethanol and 400 μl castor oil, and then mix with 7.2ml PBS, The resulting mixture was homogenized by ultrasonic mixing to obtain a suspension.

[0067] Disulfiram preparation method used in cell experiments: Disulfiram was directly dissolved in DMSO to prepare two stock solutions of 100 mM and 10 mM and stored in a -80°C refrigerator.

[0068] In this example, it was found through animal experiments that disulfiram had a certain therapeutic effect on pulmonary fibrosis, and the test methods and results were as follows:

[0069] 1 Experimental materials:

[0070] 1.1 Experimental animals: C57BL / 6 mice, male, weighing about 25g, were raised in the Experimental Animal Center of Shanghai Jiaotong University,...

Embodiment 3

[0088] Preparation of tablets:

[0089] Take 500g of disulfiram, add appropriate amount of starch to make granules, add appropriate amount of magnesium stearate, mix well and press into 1000 tablets, one tablet each time per day.

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Abstract

The invention discloses an application of disulfiram in preparation of drugs capable of resisting head and neck cancer and drugs capable of resisting liver fibrosis. According to the invention, the disulfiram is discovered to have a proliferation inhibition effect and an apoptosis promotion effect on head and neck cancer cells for the first time, and the disulfiram is proved to be a potential clinical treatment drug for head and neck cancer diseases. The head and neck cancer refers to a head and neck cancer including oral and maxillofacial tumors (oral cancer, gingival cancer and buccal cancer) and neck tumors. The disulfiram is also discovered to have a relieving effect on lung fibrosis for the first time; and the disulfiram is indicated to have an important significance on clinical treatment of the lung fibrosis and provide a new direction for treatment of the lung fibrosis. The lung fibrosis refers to idiopathic lung fibrosis and clinical secondary lung fibrosis.

Description

technical field [0001] The invention relates to the technical field of drug development and application, in particular to the use of disulfiram (tetraethylthiuram disulfide) in the preparation of anti-head and neck cancer drugs and anti-pulmonary fibrosis drugs. Background technique [0002] Head and neck cancer is a neoplastic disease characterized by malignant epithelial cells surrounding the oral cavity, pharynx, larynx, and nasal cavity. Head and neck cancer is the sixth most common cancer. The 5-year survival rate of head and neck cancer patients is only 40%-50%. There are about 650,000 new cases of head and neck cancer in the world every year, and about 350,000 patients die from head and neck cancer every year. . Related studies have shown that smoking, drinking and papillomavirus infection are the main inducing factors of head and neck cancer. The current treatment procedures for head and neck cancer are very complicated, and will cause great economic and mental pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/145A61P35/00A61P11/00
CPCA61K31/145A61P11/00A61P35/00
Inventor 钱峰孙磊姜洪超王炜伟
Owner SHANGHAI JIAO TONG UNIV
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