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Preparation method of cyclic gamma-polyglutamic acid modified hydrogel loaded with antibacterial drug

A technology of polyglutamic acid and antibacterial drugs, applied in the fields of biotechnology and pharmacy, can solve problems such as unreported, and achieve the effect of good biocompatibility

Inactive Publication Date: 2020-10-23
中国人民解放军海军特色医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But the preparation method of the cyclo-γ-PGA modified PNIPAM hydrogel drug-loading system related to load antibacterial drug, there is no report at present

Method used

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  • Preparation method of cyclic gamma-polyglutamic acid modified hydrogel loaded with antibacterial drug
  • Preparation method of cyclic gamma-polyglutamic acid modified hydrogel loaded with antibacterial drug
  • Preparation method of cyclic gamma-polyglutamic acid modified hydrogel loaded with antibacterial drug

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of cyclo-γ-PGA modified PNIPAM hydrogel

[0037] cyclo-γ-PGA ( figure 1 ) Modified PNIPAM hydrogel: Mix the two substances according to PNIPAM / cyclo-γ-PGA in order of 100:0, 75:25, 50:50, and 25:75, and the total concentration of the solution is 20% (w / v) , mixed well at room temperature for 3 hours, debubbled overnight at 4°C, and gelled in a water bath at 37°C. It can be observed that the dehydration of PNIPAM hydrogels has been effectively improved when PNIPAM / cyclo-γ-PGA is 75:25 or 50:50 ( figure 2 ). Under the scanning electron microscope, when PNIPAM / cyclo-γ-PGA was 75:25, the hydrogel showed a typical porous structure ( image 3 ).

Embodiment 2

[0038] Embodiment 2: Preparation of cyclo-γ-PGA modified PNIPAM hydrogel loaded with erythromycin

[0039] Mix erythromycin at a final concentration of 0.5 mg / ml, 150 mg / mL PNIPAM and 50 mg / mL cyclo-γ-PGA, mix thoroughly at room temperature for 3 hours, remove air bubbles overnight at 4°C, and gel in a water bath at 37°C. The water retention rate of the obtained hydrogel is 90%, which has a good water retention effect; the bacteriostasis rate is 75%, and has a good antibacterial effect.

Embodiment 3

[0040] Embodiment 3: the preparation of the cyclo-γ-PGA modified PNIPAM hydrogel of loaded neomycin

[0041] Mix neomycin, 150 mg / mL PNIPAM, and 50 mg / mL cyclo-γ-PGA at a final concentration of 1 mg / ml, mix thoroughly at room temperature for 3 hours, remove air bubbles overnight at 4°C, and gel in a water bath at 37°C. The water retention rate of the obtained hydrogel is 90%, which has a good water retention effect; the bacteriostasis rate is 80%, and has a good antibacterial effect.

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Abstract

The invention relates to the field of biotechnology and pharmaceutics, in particular to cyclic gamma-polyglutamic acid (cyclo-gamma-PGA) modified poly-N-isopropylacrylamide (PNIPAM) hydrogel loaded with an antibacterial drug and a preparation method of the hydrogel. The cyclic gamma-polyglutamic acid modified poly-N-isopropylacrylamide hydrogel is loaded with the antibacterial drug to obtain the product. The hydrogel with the three-dimensional network structure prepared by the invention has high biocompatibility, can be used as a drug loading system to load various broad-spectrum antibacterialdrugs such as erythromycin, neomycin, tetracycline and mupirocin, and is applied to the fields of biological medicines, medical auxiliary materials and the like.

Description

technical field [0001] The invention relates to the fields of biotechnology and pharmacy, in particular to a preparation method of a ring gamma-polyglutamic acid modified hydrogel loaded with antibacterial drugs. Background technique [0002] At present, bacterial infection is still a class of diseases with high morbidity and high mortality worldwide. Among them, local infection caused by burns and trauma often leads to severe local skin and soft tissue necrosis, abscess, and even systemic sepsis. Common infectious bacteria include Gram-positive bacteria such as Staphylococcus aureus and hemolytic streptococcus, Gram-negative bacteria such as Pseudomonas aeruginosa, and anaerobic bacteria. Clinical treatment mainly relies on antibacterial drugs (Poulakou G, Lagou S, Tsiodras S : What's new in the epidemiology of skin and soft tissue infections in 2018? Curr Opin in Infect Dis, 2019,32(2):77–86.). However, systemic application of antibiotics is difficult to achieve effectiv...

Claims

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Application Information

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IPC IPC(8): A61L26/00A61K9/06A61K47/42A61K47/32A61K31/7048A61K31/7036A61K31/65A61K31/351A61P31/04A61P31/02
CPCA61K9/06A61K31/351A61K31/65A61K31/7036A61K31/7048A61K47/32A61K47/42A61L26/0014A61L26/0047A61L26/0066A61L26/008A61L2300/406A61L2300/412A61L2300/602A61P31/02A61P31/04C08L89/00C08L33/24
Inventor 张黎明王蓓蕾邹帅军王博王超王倩倩柳国艳张福海
Owner 中国人民解放军海军特色医学中心
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