Preparation method of spirulina antibacterial peptide composite membrane

A technology of antimicrobial peptides and spirulina, which is applied in the field of preparation of spirulina antimicrobial peptide composite films, can solve the problems of complicated process, unsuitable for large-scale production, inconvenient storage and transportation, etc.

Active Publication Date: 2020-10-23
FUQING BRANCH OF FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, direct use of nano-titanium dioxide and film-forming agent to form a film has poor tensile strength and is easy to break, so it is not suitable for direct use as a packaging film.
[0003] Patent CN 110818956A discloses a chitosan-nano-titanium dioxide composite film, but chitosan has poor water solubility and is generally dissolved in acid, and various chemical substances need to be added to promote its dissolution, and the process is complicated
[0004] Patent CN 104497336A discloses a yellow crucian carp protein antimicrobial peptide-nanometer zinc oxide film, the price of the yellow crucian carp used is high, storage and transportation are inconvenient, and it is not suitable for large-scale production outside the place of origin

Method used

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  • Preparation method of spirulina antibacterial peptide composite membrane
  • Preparation method of spirulina antibacterial peptide composite membrane
  • Preparation method of spirulina antibacterial peptide composite membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Preparation of spirulina antimicrobial peptides: Add 6 g of spirulina powder into 120 mL of sodium phosphate buffer solution with pH=7.0, freeze and thaw three times at -20°C and thaw at room temperature, and centrifuge at 4°C for 30 min , then take the supernatant, add neutral protease in the amount of 0.0125g / 100mL, enzymolyze at 45°C for 4 hours, maintain the pH value at 7.0 during this period, heat the enzymolyzate in a water bath at 95°C for 10 minutes to inactivate the enzyme, and again After refrigerated centrifugation at 4°C for 30 min, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;

[0026] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry it, then add absolute ethanol according to the mass volume ratio of 1g:20mL, ball mill for 48 hours, and obtain nano-titanium dioxide after filtration;

[0027] 3) Preparation of composite membrane: 0.6 g of spirulina antimicrobial peptid...

Embodiment 2

[0029] 1) Preparation of spirulina antimicrobial peptides: Add 6 g of spirulina powder into 120 mL of sodium phosphate buffer solution with pH=7.0, freeze and thaw three times at -20°C and thaw at room temperature, and centrifuge at 4°C for 30 min , then take the supernatant, add neutral protease in the amount of 0.0125g / 100mL, enzymolyze at 30°C for 8 h, maintain the pH value at 7.0 during this period, heat the enzymolyzate in a water bath at 95°C for 10 min to inactivate the enzyme, and again After refrigerated centrifugation at 4°C for 30 min, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;

[0030] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry it, then add absolute ethanol according to the mass volume ratio of 1g:20mL, ball mill for 48 hours, and obtain nano-titanium dioxide after filtration;

[0031] 3) Preparation of composite film: Add 1 g of spirulina antimicrobial peptide, 2 g of ...

Embodiment 3

[0033] 1) Preparation of spirulina antimicrobial peptides: Add 6 g of spirulina powder into 120 mL of sodium phosphate buffer solution with pH=7.0, freeze and thaw three times at -20°C and thaw at room temperature, and centrifuge at 4°C for 30 min , then take the supernatant, add neutral protease in the amount of 0.0125g / 100mL, enzymolyze at 70°C for 2 h, maintain the pH value at 7.0 during this period, heat the enzymolyzate in a water bath at 95°C for 10 min to inactivate the enzyme, and again After refrigerated centrifugation at low temperature for 30 min, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;

[0034] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry it, then add absolute ethanol according to the mass volume ratio of 1g:20mL, ball mill for 48 hours, and obtain nano-titanium dioxide after filtration;

[0035] 3) Preparation of composite membrane: Add 3 g of spirulina antimicrobial ...

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Abstract

The invention discloses a preparation method of a spirulina antibacterial peptide composite membrane. The preparation method comprises the following steps: carrying out enzymolysis on spirulina powderby neutral protease to obtain spirulina antibacterial peptide, carrying out low-vacuum variable-frequency ultrasonic mixing on the spirulina antibacterial peptide, nano titanium dioxide, sodium alginate and the like, and drying to obtain the spirulina antibacterial peptide composite membrane. The prepared composite membrane is uniform in thickness, smooth in surface, free of obvious bubbles and good in antibacterial property, the tensile property of the composite membrane is greatly improved, and the composite membrane can be used as a packaging material.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a preparation method of a spirulina antibacterial peptide composite film. Background technique [0002] At present, the most commonly used packaging film materials are plastics, such as PE, PVC, PP, etc., which are not easy to degrade and have a great impact on the environment. Nano-titanium dioxide has strong stability, superhydrophilicity and non-migratory properties, as well as antibacterial, anti-aging and self-cleaning properties. However, direct use of nano-titanium dioxide and film-forming agent to form a film has poor tensile strength and is easy to break, so it is not suitable for direct use as a packaging film. [0003] Patent CN 110818956A discloses a chitosan-nano-titanium dioxide composite film, but chitosan has poor water solubility and is generally dissolved in acid, and various chemical substances need to be added to promote its dissolution, result...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L5/04C08L89/00C08L71/02C08L1/28C08K3/22C08K5/053C07K1/14C12P21/06
CPCC08J5/18C12P21/06C07K1/145C08K2201/011C08K2003/2241C08J2305/04C08J2389/00C08J2489/00C08J2471/02C08J2300/16C08J2301/28
Inventor 吴承燕张荣刚
Owner FUQING BRANCH OF FUJIAN NORMAL UNIV
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