Preparation method of spirulina antibacterial peptide composite membrane

A technology of antimicrobial peptides and spirulina, which is applied in the field of preparation of spirulina antimicrobial peptide composite films, can solve the problems of complicated process, unsuitable for large-scale production, inconvenient storage and transportation, etc.

Active Publication Date: 2020-10-23
FUQING BRANCH OF FUJIAN NORMAL UNIV
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AI-Extracted Technical Summary

Problems solved by technology

However, direct use of nano-titanium dioxide and film-forming agent to form a film has poor tensile strength and is easy to break, so it is not suitable for direct use as a packaging film.
[0003] Patent CN 110818956A discloses a chitosan-nano-titanium dioxide composite film, but chitosan has poor water solubility and is generally dissolved in acid, and various chemical su...
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Method used

Visible in conjunction with Fig. 1, the composite film surface that has added spirulina antimicrobial peptide is loose and porous, has larger adsorption area, can improve the adsorption to bacterium, thereby ca...
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Abstract

The invention discloses a preparation method of a spirulina antibacterial peptide composite membrane. The preparation method comprises the following steps: carrying out enzymolysis on spirulina powderby neutral protease to obtain spirulina antibacterial peptide, carrying out low-vacuum variable-frequency ultrasonic mixing on the spirulina antibacterial peptide, nano titanium dioxide, sodium alginate and the like, and drying to obtain the spirulina antibacterial peptide composite membrane. The prepared composite membrane is uniform in thickness, smooth in surface, free of obvious bubbles and good in antibacterial property, the tensile property of the composite membrane is greatly improved, and the composite membrane can be used as a packaging material.

Application Domain

Peptide preparation methodsFermentation

Technology Topic

Spirulina sp.Chemistry +9

Image

  • Preparation method of spirulina antibacterial peptide composite membrane
  • Preparation method of spirulina antibacterial peptide composite membrane
  • Preparation method of spirulina antibacterial peptide composite membrane

Examples

  • Experimental program(3)
  • Comparison scheme(1)
  • Effect test(1)

Example Embodiment

[0024] Example 1
[0025] 1) Preparation of spirulina antimicrobial peptides: add 6g of spirulina powder to 120mL, pH=7.0 sodium phosphate buffer solution, freeze and thaw at -20℃, thaw at room temperature three times, freeze and centrifuge at 4℃ for 30 min , Then take the supernatant, add neutral protease in the amount of 0.0125g/100mL, enzymatically hydrolyze at 45 ℃ for 4 h, during which the pH value is maintained at 7.0, the enzymatic hydrolysate is heated in a water bath at 95 ℃ for 10 min to inactivate the enzyme. After 30 minutes of freezing and centrifugation at 4°C, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;
[0026] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry, then add absolute ethanol according to the mass-volume ratio of 1g:20mL, ball mill for 48 h, and filter to obtain nano-titanium dioxide;
[0027] 3) Preparation of composite membrane: add 0.6g spirulina antimicrobial peptide, 1.2 g nano titanium dioxide, 1 g sodium alginate, 0.5 g glycerol, 0.1 g polyethylene glycol 1000 to 10 mL absolute ethanol and deionized water Dissolve in the formed composite solvent (1:20, v/v), then add the composite solvent to the total volume of the solution to 100 mL, stir and sonicate it for 30 minutes under the conditions of vacuum 100-300Pa and power 250W to make it uniform. During this period, the ultrasonic frequency is changed cyclically in the range of 40kHz~55kHz, the change method is 0.1s change once, each change is 100Hz, after ultrasonic, the solution is poured into the mold, dried at 60 ℃ for 3 h, cooled to room temperature, and then peeled off. Spirulina antimicrobial peptide composite film.

Example Embodiment

[0028] Example 2
[0029] 1) Preparation of spirulina antimicrobial peptides: add 6g of spirulina powder to 120mL, pH=7.0 sodium phosphate buffer solution, freeze and thaw at -20℃, thaw at room temperature three times, freeze and centrifuge at 4℃ for 30 min , Then take the supernatant, add neutral protease in the amount of 0.0125g/100mL, enzymatically hydrolyze at 30 ℃ for 8 h, during which the pH value is maintained at 7.0, the enzymatic hydrolysate is heated in a water bath at 95 ℃ for 10 min to inactivate the enzyme, again After 30 minutes of freezing and centrifugation at 4°C, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;
[0030] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry, then add absolute ethanol according to the mass-volume ratio of 1g:20mL, ball mill for 48 h, and filter to obtain nano-titanium dioxide;
[0031] 3) Preparation of composite membrane: 1 g spirulina antimicrobial peptide, 2 g nano titanium dioxide, 2 g gelatin, 1 g glycerol, 1 g polyethylene glycol 2000 are added to 10 mL absolute ethanol and deionized water to form Dissolve in a composite solvent (1:20, v/v), then add the composite solvent to a total volume of 100 mL. After stirring, perform ultrasonic mixing for 30 minutes under the conditions of a vacuum of 100-300 Pa and a power of 250 W. The ultrasonic frequency changes cyclically in the range of 25kHz~35kHz, the change method is 0.2s once, each change is 100Hz, after ultrasonic, the solution is poured into the mold, dried at 60 ℃ for 3 h, cooled to room temperature and then peeled off, the spirulina is obtained. Antimicrobial peptide composite membrane.

Example Embodiment

[0032] Example 3
[0033] 1) Preparation of spirulina antimicrobial peptides: add 6g of spirulina powder to 120mL, pH=7.0 sodium phosphate buffer solution, freeze and thaw at -20℃, thaw at room temperature three times, freeze and centrifuge at 4℃ for 30 min , Then take the supernatant, add neutral protease in the amount of 0.0125g/100mL, enzymolyze at 70 ℃ for 2 h, during which the pH value is maintained at 7.0, the enzymatic hydrolysate is heated in a water bath at 95 ℃ for 10 min to inactivate the enzyme, again After 30 minutes of low-temperature freezing and centrifugation, the supernatant was taken and vacuum freeze-dried to obtain the spirulina antimicrobial peptide;
[0034] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry, then add absolute ethanol according to the mass-volume ratio of 1g:20mL, ball mill for 48 h, and filter to obtain nano-titanium dioxide;
[0035] 3) Preparation of composite membrane: Add 3 g of spirulina antimicrobial peptide, 3 g of nano-titanium dioxide, 3 g of hydroxypropyl methylcellulose, 3 g of sorbitol, 1.5 g of polyethylene glycol 1000 to 10 mL of absolute ethanol and Dissolve in a composite solvent (1:20, v/v) formed by deionized water, then add the composite solvent to a total volume of 100 mL, stir and ultrasonicate for 30 minutes under the conditions of a vacuum of 100-300Pa and a power of 250W. During mixing, the ultrasonic frequency is cyclically changed in the range of 20kHz~60kHz, the change method is 0.1s change once, each change is 200Hz, after ultrasonic, the solution is poured into the mold, dried at 60 ℃ for 3 h, and then peeled off after cooling to room temperature , That is, the spirulina antimicrobial peptide composite film

PUM

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