Preparation method of spirulina antibacterial peptide composite membrane

A technology of antimicrobial peptides and spirulina, which is applied in the field of preparation of spirulina antimicrobial peptide composite films, can solve the problems of complicated process, unsuitable for large-scale production, inconvenient storage and transportation, etc.

Active Publication Date: 2020-10-23
FUQING BRANCH OF FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, direct use of nano-titanium dioxide and film-forming agent to form a film has poor tensile strength and is easy to break, so it is not suitable for direct use as a packaging film.
[0003] Patent CN 110818956A discloses a chitosan-nano-titanium dioxide composite film, but chitosan has poor water solubility and is generally dissolved in acid, and various chemical su

Method used

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  • Preparation method of spirulina antibacterial peptide composite membrane
  • Preparation method of spirulina antibacterial peptide composite membrane
  • Preparation method of spirulina antibacterial peptide composite membrane

Examples

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[0024] Example 1

[0025] 1) Preparation of spirulina antimicrobial peptides: add 6g of spirulina powder to 120mL, pH=7.0 sodium phosphate buffer solution, freeze and thaw at -20℃, thaw at room temperature three times, freeze and centrifuge at 4℃ for 30 min , Then take the supernatant, add neutral protease in the amount of 0.0125g / 100mL, enzymatically hydrolyze at 45 ℃ for 4 h, during which the pH value is maintained at 7.0, the enzymatic hydrolysate is heated in a water bath at 95 ℃ for 10 min to inactivate the enzyme. After 30 minutes of freezing and centrifugation at 4°C, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;

[0026] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry, then add absolute ethanol according to the mass-volume ratio of 1g:20mL, ball mill for 48 h, and filter to obtain nano-titanium dioxide;

[0027] 3) Preparation of composite membrane: add 0.6g spirulina antimicrobial pepti...

Example Embodiment

[0028] Example 2

[0029] 1) Preparation of spirulina antimicrobial peptides: add 6g of spirulina powder to 120mL, pH=7.0 sodium phosphate buffer solution, freeze and thaw at -20℃, thaw at room temperature three times, freeze and centrifuge at 4℃ for 30 min , Then take the supernatant, add neutral protease in the amount of 0.0125g / 100mL, enzymatically hydrolyze at 30 ℃ for 8 h, during which the pH value is maintained at 7.0, the enzymatic hydrolysate is heated in a water bath at 95 ℃ for 10 min to inactivate the enzyme, again After 30 minutes of freezing and centrifugation at 4°C, the supernatant was vacuum freeze-dried to obtain the spirulina antimicrobial peptide;

[0030] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry, then add absolute ethanol according to the mass-volume ratio of 1g:20mL, ball mill for 48 h, and filter to obtain nano-titanium dioxide;

[0031] 3) Preparation of composite membrane: 1 g spirulina antimicrobial pept...

Example Embodiment

[0032] Example 3

[0033] 1) Preparation of spirulina antimicrobial peptides: add 6g of spirulina powder to 120mL, pH=7.0 sodium phosphate buffer solution, freeze and thaw at -20℃, thaw at room temperature three times, freeze and centrifuge at 4℃ for 30 min , Then take the supernatant, add neutral protease in the amount of 0.0125g / 100mL, enzymolyze at 70 ℃ for 2 h, during which the pH value is maintained at 7.0, the enzymatic hydrolysate is heated in a water bath at 95 ℃ for 10 min to inactivate the enzyme, again After 30 minutes of low-temperature freezing and centrifugation, the supernatant was taken and vacuum freeze-dried to obtain the spirulina antimicrobial peptide;

[0034] 2) Preparation of nano-titanium dioxide: wash the titanium dioxide with deionized water and dry, then add absolute ethanol according to the mass-volume ratio of 1g:20mL, ball mill for 48 h, and filter to obtain nano-titanium dioxide;

[0035] 3) Preparation of composite membrane: Add 3 g of spirulina antim...

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Abstract

The invention discloses a preparation method of a spirulina antibacterial peptide composite membrane. The preparation method comprises the following steps: carrying out enzymolysis on spirulina powderby neutral protease to obtain spirulina antibacterial peptide, carrying out low-vacuum variable-frequency ultrasonic mixing on the spirulina antibacterial peptide, nano titanium dioxide, sodium alginate and the like, and drying to obtain the spirulina antibacterial peptide composite membrane. The prepared composite membrane is uniform in thickness, smooth in surface, free of obvious bubbles and good in antibacterial property, the tensile property of the composite membrane is greatly improved, and the composite membrane can be used as a packaging material.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a preparation method of a spirulina antibacterial peptide composite film. Background technique [0002] At present, the most commonly used packaging film materials are plastics, such as PE, PVC, PP, etc., which are not easy to degrade and have a great impact on the environment. Nano-titanium dioxide has strong stability, superhydrophilicity and non-migratory properties, as well as antibacterial, anti-aging and self-cleaning properties. However, direct use of nano-titanium dioxide and film-forming agent to form a film has poor tensile strength and is easy to break, so it is not suitable for direct use as a packaging film. [0003] Patent CN 110818956A discloses a chitosan-nano-titanium dioxide composite film, but chitosan has poor water solubility and is generally dissolved in acid, and various chemical substances need to be added to promote its dissolution, result...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L5/04C08L89/00C08L71/02C08L1/28C08K3/22C08K5/053C07K1/14C12P21/06
CPCC08J5/18C12P21/06C07K1/145C08K2201/011C08K2003/2241C08J2305/04C08J2389/00C08J2489/00C08J2471/02C08J2300/16C08J2301/28
Inventor 吴承燕张荣刚
Owner FUQING BRANCH OF FUJIAN NORMAL UNIV
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