Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting antiplatelet drug in blood plasma by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

An anti-platelet drug and ultra-high performance liquid phase technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that there are no simultaneous detection of six compounds, and achieve simple pretreatment process, precision and accuracy, good selectivity

Active Publication Date: 2020-10-23
南京品生医学检验实验室有限公司
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, after a comprehensive analysis of domestic and foreign literature and patents, the detection of these antiplatelet drugs is only one or two compounds at the same time, and there is no report on the simultaneous detection of these six compounds.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting antiplatelet drug in blood plasma by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Method for detecting antiplatelet drug in blood plasma by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Method for detecting antiplatelet drug in blood plasma by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] 1. Experimental materials and instruments

[0086] 1. Materials

[0087] The samples of the methodological research experiment came from the plasma samples collected from the outpatient clinic of Nanjing Drum Tower Hospital in December 2019.

[0088](1) Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA) ; ultrapure water instrument (ELGA LabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glassware, graduated cylinder, etc. . .

[0089] (2) Reagent consumables: MS grade methanol (Fisher, USA); MS grade acetonitrile (Fisher, USA); HPLC grade acetonitrile (Honeywell, USA); MS grade formic acid (Fisher, USA); HPLC grade methanol (Honeywell, USA) ; Chromatographic column: Waters BEH C18 (2.1×100 mm, 1.7 μm). ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for detecting an antiplatelet drug in blood plasma by using an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The method has the advantages that the pretreatment process is simple, the cost is low, the sensitivity is high, the specificity is high, separation and detection of the antiplatelet drug are completed within 5 min, the accuracy degree and precision basically meet the requirements, the method can be used for quantitative analysis of the clinical antiplatelet drug, and a reliable detection method is provided for monitoring the treatment concentration of the clinical antiplatelet drug.

Description

technical field [0001] The invention belongs to the technical field of blood plasma detection, and in particular relates to a method for detecting antiplatelet drugs in blood plasma by ultra-high performance liquid chromatography tandem mass spectrometry. Deshydroxyethoxy Ticagrelor (DHTIC), aspirin (Acetylsalicylic acid, ASA), salicylic acid (Salicylic acid, SA), clopidogrel (Clopidogrel, CLOP) and clopidogrel carboxylic acid metabolite (Clopidogrel Carboxylic Acid, CLOPC) . Background technique [0002] Aspirin is a commonly used non-steroidal drug, which was originally used for antipyretic and analgesic. Later studies found that the use of small doses of aspirin can inhibit the aggregation of platelets, reduce the adhesion rate of platelets, and prevent the formation of thrombus. Oral aspirin 75mg is used as a preventive drug, and is widely used in the prevention of ischemic cardiovascular disease, and can be used in patients with myocardial infarction when used in low d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/7266G01N2030/045
Inventor 成晓亮李美娟
Owner 南京品生医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com