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A 3D printed bone tissue engineering scaffold with slow-release and osteogenesis-promoting functions, its preparation method and application

A tissue engineering scaffold and 3D printing technology, applied in the field of biomedical materials, can solve the problems of slow healing of bone tissue and poor osteogenic performance, and achieve good biocompatibility, good bone repair effect, and good biological activity.

Active Publication Date: 2021-11-30
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bone tissue has a typical three-dimensional porous structure, which is especially suitable for 3D printing technology. 3D printed bone tissue engineering scaffolds have been used clinically, but there are still some shortcomings, such as poor bone formation performance, which leads to slow healing of bone tissue after implantation.

Method used

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  • A 3D printed bone tissue engineering scaffold with slow-release and osteogenesis-promoting functions, its preparation method and application
  • A 3D printed bone tissue engineering scaffold with slow-release and osteogenesis-promoting functions, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of 3D printing ink

[0048] In a water bath at 30°C, add sodium alginate powder material to deionized water to prepare a 6% sodium alginate solution, add 1ml of icariin-dimethyl sulfoxide solution with a concentration of 0.1g / ml, and use a magnetic Mix it well with a mixer until smooth. After preheating at 50°C for 30-60 minutes, reduce the viscosity of the sodium alginate solution, then slowly add the solid calcium phosphate powder into the preheated solution, and mix the slurry evenly with a homogeneous mixer until no phosphoric acid is observed Calcium agglomerates. The mass ratio of calcium phosphate, sodium alginate, icariin and deionized water in the final slurry is 25:6:0.08:100. This mixed slurry is used as 3D printing ink.

[0049] (2) printing support

[0050] The three-dimensional model of the scaffold was designed by 3D modeling software, and the mixed ink was printed into a calcium phosphate scaffold with a first-level macroporous structur...

Embodiment 2

[0062] The 3D printed bone tissue engineering scaffold material was prepared with reference to the method described in Example 1, which differs from Example 1 in that the mass ratio of calcium phosphate, sodium alginate, icariin and deionized water was adjusted to 25:6 : 0.04: 100, all the other processing methods are the same as those described in Example 1. The experimental results show that the obtained scaffold has a slow-release function and an osteogenesis-promoting effect, but compared with Example 1, due to the lower solid content of icariin in the printing paste, bone tissue with slow-release and osteogenesis-promoting functions The total drug loading of the engineering scaffold is less, and the osteogenesis-promoting function of the scaffold obtained in this embodiment on bone defects is better than that of Comparative Example 1, but slightly worse than that of Example 1.

Embodiment 3

[0064] The 3D printed bone tissue engineering scaffold material was prepared with reference to the method described in Example 1, which differs from Example 1 in that the mass ratio of calcium phosphate, sodium alginate, icariin and deionized water was adjusted to 25:6 : 0.16: 100, all the other processing methods are the same as those described in Example 1. The experimental results show that the obtained scaffold has slow-release function and osteogenesis-promoting effect. Since the solid content of icariin in the printing slurry is more than that of Examples 1 and 2, the bone tissue engineering scaffold with slow-release and osteogenesis-promoting function The total amount of drug loaded is more, and the bone defect-promoting function of the scaffold obtained in this embodiment is better than that of Examples 1 and 2.

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Abstract

The invention discloses a 3D printed bone tissue engineering scaffold with functions of sustained release and osteogenesis promotion and a preparation method thereof, belonging to the technical field of biomedical materials. The scaffold is formed by in-situ cross-linking of porous calcium phosphate material and sodium alginate, and simultaneously loads drug icariin. The bone tissue engineering scaffold prepared by the present invention has good biocompatibility and bioactivity. At the same time, by adding icariin drug to the printing ink, the osteogenic performance of the scaffold is further enhanced, and finally regulated by 3D printing and post-processing The pore structure of the scaffold, the cross-linking degree of the scaffold can be adjusted by changing the concentration of the calcium chloride cross-linking agent and the cross-linking time, and then the degradation rate of the material in vivo can be adjusted. The sustained drug release effect of the stent is realized by changing the drug concentration to regulate the drug loading of the stent. The stent material of the present invention can be used as artificial bone and bone tissue engineering reconstruction and repair, and has broad clinical application prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a 3D printed bone tissue engineering scaffold loaded with icariin for slow release to promote osteoinductive reconstruction and a preparation method thereof. [0002] technical background [0003] Bone tissue engineering scaffold is an important part of tissue engineering. It is to transplant the seed cells cultured in vitro onto the scaffold material, and then implant the material into the bone defect, supplemented by different measures to promote cell proliferation and differentiation, and achieve the purpose of repairing bone defect. . An ideal bone scaffold material should be able to simulate biological functions such as bone composition, structure, and mechanical properties, provide a temporary place for cell proliferation and differentiation, and guide cells and blood vessels to grow and multiply along the pores of the scaffold. Traditional bone sca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/56A61L27/54A61L27/20A61L27/50A61L27/58B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/12A61L27/20A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/112A61L2300/23A61L2300/412A61L2300/602A61L2430/02B33Y10/00B33Y80/00B33Y70/10C08L5/04
Inventor 周长春张宸熙孙桓樊渝江梁洁雷皓远吴丽娜孙建勋肖玉梅蒋青张兴栋
Owner SICHUAN UNIV
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